TY - JOUR
T1 - Changes in expression of prosaposin in the rat facial nerve nucleus after facial nerve transection
AU - Unuma, Kana
AU - Chen, Jie
AU - Saito, Shouichiro
AU - Kobayashi, Naoto
AU - Sato, Kohji
AU - Saito, Kyoko
AU - Wakisaka, Hiroyuki
AU - Mominoki, Katsumi
AU - Sano, Akira
AU - Matsuda, Seiji
N1 - Funding Information:
We thank S. Masuda (INCS, Ehime University) and C. Kanaka (Department of Anatomy and Neuroscience, Hamamatsu University School of Medicine) for technical instruction. We also thank Dr. K. Miyawaki (Division of Comparative Pathology, Center of Marine Environmental Studies, Ehime University) for fruitful advice. This study was supported in part by the grants-in-aid for Scientific Research (B) (No. 14370466 to SM) and for Young Scientists (B) (No. 16791004 to SS) from the Japan Society for the Promotion of Science.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/7
Y1 - 2005/7
N2 - Prosaposin is the precursor of saposins A, B, C and D, which are activators of sphingolipid hydrolases. In addition, unprocessed prosaposin functions as a neurotrophic factor in the central and peripheral nervous systems by acting to prevent neuronal apoptosis, to elongate neurites and to facilitate myelination. In this study, the expression pattern of prosaposin in the facial nerve nucleus after facial nerve transection was examined by immunohistochemistry and in situ hybridization. Prosaposin immunoreactivity in the neurons on the operated side facial nerve nucleus showed a biphasic pattern: it was significantly increased on day 3 after transection, decreased dramatically on day 7, started to increase gradually on day 14 and reached another peak on day 21 after transection. Significant increases in the levels of prosaposin mRNA were identified in the neurons on the operated side, suggesting that prosaposin was synthesized vigorously by the neurons themselves in the case of facial nerve transection. The diverse changes in prosaposin immunoreactivity during the process of facial nerve regeneration may reflect the diverse neurotrophic activities of prosaposin in facial motoneurons.
AB - Prosaposin is the precursor of saposins A, B, C and D, which are activators of sphingolipid hydrolases. In addition, unprocessed prosaposin functions as a neurotrophic factor in the central and peripheral nervous systems by acting to prevent neuronal apoptosis, to elongate neurites and to facilitate myelination. In this study, the expression pattern of prosaposin in the facial nerve nucleus after facial nerve transection was examined by immunohistochemistry and in situ hybridization. Prosaposin immunoreactivity in the neurons on the operated side facial nerve nucleus showed a biphasic pattern: it was significantly increased on day 3 after transection, decreased dramatically on day 7, started to increase gradually on day 14 and reached another peak on day 21 after transection. Significant increases in the levels of prosaposin mRNA were identified in the neurons on the operated side, suggesting that prosaposin was synthesized vigorously by the neurons themselves in the case of facial nerve transection. The diverse changes in prosaposin immunoreactivity during the process of facial nerve regeneration may reflect the diverse neurotrophic activities of prosaposin in facial motoneurons.
KW - Facial motoneurons
KW - Immunohistochemistry
KW - In situ hybridization
KW - Neurotrophic factor
KW - Prosaposin
KW - Regeneration
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U2 - 10.1016/j.neures.2005.03.009
DO - 10.1016/j.neures.2005.03.009
M3 - Article
C2 - 15927723
AN - SCOPUS:20144365037
VL - 52
SP - 220
EP - 227
JO - Neuroscience Research
JF - Neuroscience Research
SN - 0168-0102
IS - 3
ER -