Abstract
Prosaposin is the precursor of saposins A, B, C and D, which are activators of sphingolipid hydrolases. In addition, unprocessed prosaposin functions as a neurotrophic factor in the central and peripheral nervous systems by acting to prevent neuronal apoptosis, to elongate neurites and to facilitate myelination. In this study, the expression pattern of prosaposin in the facial nerve nucleus after facial nerve transection was examined by immunohistochemistry and in situ hybridization. Prosaposin immunoreactivity in the neurons on the operated side facial nerve nucleus showed a biphasic pattern: it was significantly increased on day 3 after transection, decreased dramatically on day 7, started to increase gradually on day 14 and reached another peak on day 21 after transection. Significant increases in the levels of prosaposin mRNA were identified in the neurons on the operated side, suggesting that prosaposin was synthesized vigorously by the neurons themselves in the case of facial nerve transection. The diverse changes in prosaposin immunoreactivity during the process of facial nerve regeneration may reflect the diverse neurotrophic activities of prosaposin in facial motoneurons.
| Original language | English |
|---|---|
| Pages (from-to) | 220-227 |
| Number of pages | 8 |
| Journal | Neuroscience Research |
| Volume | 52 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jul 2005 |
| Externally published | Yes |
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Keywords
- Facial motoneurons
- Immunohistochemistry
- In situ hybridization
- Neurotrophic factor
- Prosaposin
- Regeneration
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Changes in expression of prosaposin in the rat facial nerve nucleus after facial nerve transection. / Unuma, Kana; Chen, Jie; Saito, Shouichiro; Kobayashi, Naoto; Sato, Kohji; Saito, Kyoko; Wakisaka, Hiroyuki; Mominoki, Katsumi; Sano, Akira; Matsuda, Seiji.
In: Neuroscience Research, Vol. 52, No. 3, 07.2005, p. 220-227.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Changes in expression of prosaposin in the rat facial nerve nucleus after facial nerve transection
AU - Unuma, Kana
AU - Chen, Jie
AU - Saito, Shouichiro
AU - Kobayashi, Naoto
AU - Sato, Kohji
AU - Saito, Kyoko
AU - Wakisaka, Hiroyuki
AU - Mominoki, Katsumi
AU - Sano, Akira
AU - Matsuda, Seiji
PY - 2005/7
Y1 - 2005/7
N2 - Prosaposin is the precursor of saposins A, B, C and D, which are activators of sphingolipid hydrolases. In addition, unprocessed prosaposin functions as a neurotrophic factor in the central and peripheral nervous systems by acting to prevent neuronal apoptosis, to elongate neurites and to facilitate myelination. In this study, the expression pattern of prosaposin in the facial nerve nucleus after facial nerve transection was examined by immunohistochemistry and in situ hybridization. Prosaposin immunoreactivity in the neurons on the operated side facial nerve nucleus showed a biphasic pattern: it was significantly increased on day 3 after transection, decreased dramatically on day 7, started to increase gradually on day 14 and reached another peak on day 21 after transection. Significant increases in the levels of prosaposin mRNA were identified in the neurons on the operated side, suggesting that prosaposin was synthesized vigorously by the neurons themselves in the case of facial nerve transection. The diverse changes in prosaposin immunoreactivity during the process of facial nerve regeneration may reflect the diverse neurotrophic activities of prosaposin in facial motoneurons.
AB - Prosaposin is the precursor of saposins A, B, C and D, which are activators of sphingolipid hydrolases. In addition, unprocessed prosaposin functions as a neurotrophic factor in the central and peripheral nervous systems by acting to prevent neuronal apoptosis, to elongate neurites and to facilitate myelination. In this study, the expression pattern of prosaposin in the facial nerve nucleus after facial nerve transection was examined by immunohistochemistry and in situ hybridization. Prosaposin immunoreactivity in the neurons on the operated side facial nerve nucleus showed a biphasic pattern: it was significantly increased on day 3 after transection, decreased dramatically on day 7, started to increase gradually on day 14 and reached another peak on day 21 after transection. Significant increases in the levels of prosaposin mRNA were identified in the neurons on the operated side, suggesting that prosaposin was synthesized vigorously by the neurons themselves in the case of facial nerve transection. The diverse changes in prosaposin immunoreactivity during the process of facial nerve regeneration may reflect the diverse neurotrophic activities of prosaposin in facial motoneurons.
KW - Facial motoneurons
KW - Immunohistochemistry
KW - In situ hybridization
KW - Neurotrophic factor
KW - Prosaposin
KW - Regeneration
UR - http://www.scopus.com/inward/record.url?scp=20144365037&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20144365037&partnerID=8YFLogxK
U2 - 10.1016/j.neures.2005.03.009
DO - 10.1016/j.neures.2005.03.009
M3 - Article
C2 - 15927723
AN - SCOPUS:20144365037
VL - 52
SP - 220
EP - 227
JO - Neuroscience Research
JF - Neuroscience Research
SN - 0168-0102
IS - 3
ER -