Changes in c-fos induction in dorsal horn neurons by hindpaw formalin stimulation following tibial neurotomy

Tomosada Sugimoto, Hiroyuki Ichikawa, Seiji Mitani, Akemi Hitsu, Nakago Tadao

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The hindpaw was partially denervated by the tibial nerve transection in adult rats. At post-transection intervals varying from 2 to 168 days, the hindpaw was stimulated bilaterally by subcutaneous injection of formalin. The excitability of dorsal horn neurons was expressed as the percentage ratio of the number of formalin-induced c-fos protein-like immunoreactive neurons (fos-neurons) on the neurotomized (experimental) side to that on the un-neurotomized (control) side. At 2 days post-injury, a marked reduction in the number of fos-neurons was noted in laminae I-VII of the lumbar spinal cord. Among these, reduction was greatest in the medial 3 8 of laminae I and II (terminal field of the tibial nerve, i.e. tibial territory), and smallest in the lateral 5 8 of the same laminae (the peroneal/hip territory). The low level of c-fos induction remained unchanged for 7 days. At 14 days, the excitability of neurons in all laminae showed a marked increase compared to the post-injury days 2 and 3 combined. Thereafter, the increased level of excitability in the tibial territory was maintained throughout the post-injury period examined in this study. On the other hand, a statistically significant increase in excitability in the peroneal/hip territory was only seen between 14 and 28 days and the excitability almost returned to the baseline (2 days and 3 post-transection combined) level at 42 days. Although deeper laminae (III-VII) contained much less formalin-induced fos-neurons, they also exhibited post-injury excitability changes with a temporal pattern similar to that of the peroneal/hip territory of laminae I and II. Possible mechanisms underlying the post-injury hypersensitivity are discussed in the light of peripheral and central changes following peripheral nerve injuries.

Original languageEnglish
Pages (from-to)348-354
Number of pages7
JournalBrain Research
Volume642
Issue number1-2
DOIs
Publication statusPublished - Apr 11 1994

Fingerprint

Posterior Horn Cells
Formaldehyde
Substantia Gelatinosa
Wounds and Injuries
Neurons
Hip
Tibial Nerve
Spinal Cord
Proto-Oncogene Proteins c-fos
Peripheral Nerve Injuries
Subcutaneous Injections
Hypersensitivity
Proteins
Spinal Cord Dorsal Horn

Keywords

  • c-fos
  • Dorsal horn
  • Peripheral nerve injury
  • Peroneal nerve
  • Somatotopy
  • Tibial nerve

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Changes in c-fos induction in dorsal horn neurons by hindpaw formalin stimulation following tibial neurotomy. / Sugimoto, Tomosada; Ichikawa, Hiroyuki; Mitani, Seiji; Hitsu, Akemi; Tadao, Nakago.

In: Brain Research, Vol. 642, No. 1-2, 11.04.1994, p. 348-354.

Research output: Contribution to journalArticle

Sugimoto, Tomosada ; Ichikawa, Hiroyuki ; Mitani, Seiji ; Hitsu, Akemi ; Tadao, Nakago. / Changes in c-fos induction in dorsal horn neurons by hindpaw formalin stimulation following tibial neurotomy. In: Brain Research. 1994 ; Vol. 642, No. 1-2. pp. 348-354.
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abstract = "The hindpaw was partially denervated by the tibial nerve transection in adult rats. At post-transection intervals varying from 2 to 168 days, the hindpaw was stimulated bilaterally by subcutaneous injection of formalin. The excitability of dorsal horn neurons was expressed as the percentage ratio of the number of formalin-induced c-fos protein-like immunoreactive neurons (fos-neurons) on the neurotomized (experimental) side to that on the un-neurotomized (control) side. At 2 days post-injury, a marked reduction in the number of fos-neurons was noted in laminae I-VII of the lumbar spinal cord. Among these, reduction was greatest in the medial 3 8 of laminae I and II (terminal field of the tibial nerve, i.e. tibial territory), and smallest in the lateral 5 8 of the same laminae (the peroneal/hip territory). The low level of c-fos induction remained unchanged for 7 days. At 14 days, the excitability of neurons in all laminae showed a marked increase compared to the post-injury days 2 and 3 combined. Thereafter, the increased level of excitability in the tibial territory was maintained throughout the post-injury period examined in this study. On the other hand, a statistically significant increase in excitability in the peroneal/hip territory was only seen between 14 and 28 days and the excitability almost returned to the baseline (2 days and 3 post-transection combined) level at 42 days. Although deeper laminae (III-VII) contained much less formalin-induced fos-neurons, they also exhibited post-injury excitability changes with a temporal pattern similar to that of the peroneal/hip territory of laminae I and II. Possible mechanisms underlying the post-injury hypersensitivity are discussed in the light of peripheral and central changes following peripheral nerve injuries.",
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