Changes in annexin I and II levels during the postnatal development of rat pancreatic islets

Makoto Ohnishi, Masaaki Tokuda, Tsutomu Masaki, Takashi Fujimura, Yuji Tai, Hideki Matsui, Toshifumi Itano, Toshihiko Ishida, Jiro Takahara, Ryoji Konishi, Osamu Hatase

Research output: Contribution to journalArticle

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Abstract

The expression patterns and the dynamic changes in content of both annexin I and annexin II in the rat pancreatic islets during postnatal development were investigated by both western blot analysis and immunohistochemistry. Immunohistochemical methods clearly demonstrated the presence of annexins I and II exclusively in pancreatic islets, while exocrine tissues were not stained by anti-annexin antibodies. Pancreatic islets were diffusely stained with no specific differences in distribution between different cell types. The expression of annexin I in pancreatic islets gradually increased with postnatal development. A developmental study of annexins I and II by western blot analysis essentially supported the results obtained by immunohistochemistry. In addition, the increasing expression of two protein tyrosine kinases, epidermal growth factor-receptor/kinase and pp60(src), which phosphorylate annexin I and annexin II, respectively, and of protein kinase C, which phosphorylates both proteins, was also shown during postnatal development in rat pancreatic islets. Thus, a relationship between the expression of annexins I and II and the maturation of islet cell function is suggested.

Original languageEnglish
Pages (from-to)2117-2125
Number of pages9
JournalJournal of Cell Science
Volume107
Issue number8
Publication statusPublished - Aug 1994
Externally publishedYes

Fingerprint

Annexin A2
Annexin A1
Islets of Langerhans
Western Blotting
Immunohistochemistry
Annexins
Epidermal Growth Factor Receptor
Protein-Tyrosine Kinases
Protein Kinase C
Anti-Idiotypic Antibodies

Keywords

  • Annexin I
  • Annexin II
  • Development
  • Islets
  • Pancreas

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ohnishi, M., Tokuda, M., Masaki, T., Fujimura, T., Tai, Y., Matsui, H., ... Hatase, O. (1994). Changes in annexin I and II levels during the postnatal development of rat pancreatic islets. Journal of Cell Science, 107(8), 2117-2125.

Changes in annexin I and II levels during the postnatal development of rat pancreatic islets. / Ohnishi, Makoto; Tokuda, Masaaki; Masaki, Tsutomu; Fujimura, Takashi; Tai, Yuji; Matsui, Hideki; Itano, Toshifumi; Ishida, Toshihiko; Takahara, Jiro; Konishi, Ryoji; Hatase, Osamu.

In: Journal of Cell Science, Vol. 107, No. 8, 08.1994, p. 2117-2125.

Research output: Contribution to journalArticle

Ohnishi, M, Tokuda, M, Masaki, T, Fujimura, T, Tai, Y, Matsui, H, Itano, T, Ishida, T, Takahara, J, Konishi, R & Hatase, O 1994, 'Changes in annexin I and II levels during the postnatal development of rat pancreatic islets', Journal of Cell Science, vol. 107, no. 8, pp. 2117-2125.
Ohnishi M, Tokuda M, Masaki T, Fujimura T, Tai Y, Matsui H et al. Changes in annexin I and II levels during the postnatal development of rat pancreatic islets. Journal of Cell Science. 1994 Aug;107(8):2117-2125.
Ohnishi, Makoto ; Tokuda, Masaaki ; Masaki, Tsutomu ; Fujimura, Takashi ; Tai, Yuji ; Matsui, Hideki ; Itano, Toshifumi ; Ishida, Toshihiko ; Takahara, Jiro ; Konishi, Ryoji ; Hatase, Osamu. / Changes in annexin I and II levels during the postnatal development of rat pancreatic islets. In: Journal of Cell Science. 1994 ; Vol. 107, No. 8. pp. 2117-2125.
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