Changes in androgen-producing cell size and circulating 11-ketotestosterone level during female-male sex change of honeycomb grouper Epinephelus merra

Mohammad Ashraful Alam, Ramji Kumar Bhandari, Yasuhisa Kobayashi, Shigeo Nakamura, Kiyoshi Soyano, Masaru Nakamura

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

11-ketotestosterone (11-KT), a potent male-specific androgen in fish, has important roles on spermatogenesis, male behavior, and nuptial coloration. The site of 11-KT synthesis and its role on male germ cell development during protogynous sex change is not clearly understood. We examined the dynamics of steroidogenic enzymes immunolocalization, viz cholesterol side-chain cleavage (P450scc), biomarker of steroids and cytochrome P45011β-hydroxylase (P45011β), downstream to 11-KT production, throughout the process of sex change in honeycomb grouper (Epinephelus merra). In female, P450scc immunoreactivity (-ir) was observed in the theca layer and tunica near blood vessels (BV). During the onset of sex change, P450scc reactive cells were observed in the remaining follicle layer of degenerated oocyte of the ovo-testis in early transitional (ET) and late transitional (LT). In male, P450scc-ir was localized in the interstitial Legdig cells of testis. P45011β reactive cells were observed in the tunica near BV in female but not in theca layer. In ET and LT phases gonads, P45011β localized in remaining follicle layer of degenerated oocyte and tunica near BV. On the other hand, in male, both interstices and tunica near BV showed strong signals against P45011β. Moreover, in vivo and in vitro levels of 11-KT related with the changes in the nuclei diameter of P45011β-positive cells in both tunica near BV and remaining follicle layer of degenerated oocyte to interstices during the progress of sex change. The present results suggest that 11-KT produced in the tunica near BV may provide the stimulus for female to degenerate oocytes and initiate sex change. However, 11-KT produced both in tunica near BV and remaining follicle layer of degenerated oocyte possibly plays critical role during testicular differentiation as well as gonadal restructuring at mid to late phases (ET to LT) of sex change in honeycomb grouper.

Original languageEnglish
Pages (from-to)206-214
Number of pages9
JournalMolecular Reproduction and Development
Volume73
Issue number2
DOIs
Publication statusPublished - Feb 2006
Externally publishedYes

Fingerprint

Cell Size
Androgens
Blood Vessels
Oocytes
Testis
Gonads
Spermatogenesis
Cytochromes
Mixed Function Oxygenases
11-ketotestosterone
Germ Cells
Fishes
Biomarkers
Steroids
Cholesterol
Enzymes

Keywords

  • 11-KT
  • Honeycomb grouper
  • P45011β
  • P450scc
  • Sex change

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Changes in androgen-producing cell size and circulating 11-ketotestosterone level during female-male sex change of honeycomb grouper Epinephelus merra. / Alam, Mohammad Ashraful; Bhandari, Ramji Kumar; Kobayashi, Yasuhisa; Nakamura, Shigeo; Soyano, Kiyoshi; Nakamura, Masaru.

In: Molecular Reproduction and Development, Vol. 73, No. 2, 02.2006, p. 206-214.

Research output: Contribution to journalArticle

Alam, Mohammad Ashraful ; Bhandari, Ramji Kumar ; Kobayashi, Yasuhisa ; Nakamura, Shigeo ; Soyano, Kiyoshi ; Nakamura, Masaru. / Changes in androgen-producing cell size and circulating 11-ketotestosterone level during female-male sex change of honeycomb grouper Epinephelus merra. In: Molecular Reproduction and Development. 2006 ; Vol. 73, No. 2. pp. 206-214.
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