Change of glutathione peroxidase synthesis along with that of superoxide dismutase synthesis in mice spleens after low-dose X-ray irradiation

Kiyonori Yamaoka, Shuji Kojima, Mareyuki Takahashi, Takaharu Nomura, Keiji Iriyama

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

We have previously demonstrated that the activity of superoxide dismutase (SOD), an antioxidant, is enhanced by low-dose X-ray irradiation in various organs of animals such as rats. Since SOD is an enzyme that mediates the dismutation of O2- to H2O2, the question as to whether the resultant H2O2 is further detoxicated into H2O and O2 or not must still be evaluated. Hence, we studied the effect of low-dose X-ray irradiation on the synthesis of glutathione peroxidase (GSHPx), which is an antioxidant that catalyzes this reaction. The results suggest that H2O2 produced by increased SOD activity can be detoxicated into H2O and O2 due to simultaneous enhancement of the GSHPx activity by X-ray irradiation at 20 cGy, in contrast to irradiation at 400 cGy. The results also show the enhancement in enzyme activities by induction of their synthesis shortly after irradiation at 20 cGy. Moreover, as this phenomenon was observed in BALB/c mice (which are more radiation-sensitive compared to other mouse strains) and radiation-resistant C57BL/6NJcl mice, it was considered to be a common phenomenon in the rat spleen. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)265-270
Number of pages6
JournalBiochimica et Biophysica Acta - General Subjects
Volume1381
Issue number2
DOIs
Publication statusPublished - Jul 23 1998
Externally publishedYes

Fingerprint

Glutathione Peroxidase
Superoxide Dismutase
Dosimetry
Spleen
X-Rays
Irradiation
X rays
Antioxidants
Radiation
Animal Structures
Enzyme Induction
Rats
Inbred C57BL Mouse
Enzyme activity
Enzymes
Animals

Keywords

  • Glutathione peroxidase
  • Low-dose X-irradiation
  • Spleen
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Change of glutathione peroxidase synthesis along with that of superoxide dismutase synthesis in mice spleens after low-dose X-ray irradiation. / Yamaoka, Kiyonori; Kojima, Shuji; Takahashi, Mareyuki; Nomura, Takaharu; Iriyama, Keiji.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1381, No. 2, 23.07.1998, p. 265-270.

Research output: Contribution to journalArticle

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