Change of epigenetic control of cystathionine beta-synthase gene expression through dietary vitamin B12 is not recovered by methionine supplementation

Atsushi Uekawa, Keishuke Katsushima, Asumi Ogata, Tetsunori Kawata, Nozomi Maeda, Ken Ichi Kobayashi, Akio Maekawa, Tadahiro Tadokoro, Yuji Yamamoto

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18 Citations (Scopus)

Abstract

Background/Aims: Vitamin B12 (B-12) is an essential cofactor for methionine synthase, and methionine is critical for the methylation of various biological molecules including DNA. Whether changes in B-12 levels can alter specific gene expression through DNA methylation and whether dietary methionine has any effect on general DNA methylation status still remains controversial. Methods: We raised severely B-12-deficient rats as well severely-B-12 deficient rats but supplemented with 5% methionine. mRNA levels of methionine cycle-related enzymes were analyzed. Results: Gene expression patterns changed under B-12-deficient conditions but were recovered by dietary methionine supplementation to B-12-deficient rats. However, cystathionine β-synthase mRNA levels, which had decreased under B-12-deficient conditions, did not recover with supplementary dietary methionine. The CpG island of the cystathionine β-synthase promoter was hypomethylated in B-12-deficient rats, and showed no recovery after methionine addition. Conclusions: Dietary B-12 can affect epigenetic machinery by regulating DNA methylation status and dietary methionine may have small effects on DNA methylation.

Original languageEnglish
Pages (from-to)29-36
Number of pages8
JournalJournal of Nutrigenetics and Nutrigenomics
Volume2
Issue number1
DOIs
Publication statusPublished - Feb 1 2009

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Keywords

  • DNA methylation
  • Epigenetic control
  • Methionine cycle
  • Vitamin B

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science
  • Genetics

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