Change of central cholinergic receptors following lesions of nucleus basalis magnocellularis in rats: Search for an imaging index suitable for the early detection of Alzheimer's disease

Mikako Ogawa, Yasuhiko Iida, Masaki Nakagawa, Yugi Kuge, Hidekazu Kawashima, Akiko Tominaga, Masashi Ueda, Yasuhiro Magata, Hideo Saji

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Cholinergic system in the central nervous system is involved in the memory function. Thus, because the dysfunction of cholinergic system that project to the cerebral cortex from nucleus basalis of Meynert (nbM) would be implicated in the memory function deficits in Alzheimer's disease (AD), evaluating cholinergic function may be useful for the early detection of AD. In this study, because the nucleus basalis magnocellularis (NBM) in rats is equivalent to nbM in human, we investigated the change in cholinergic receptors in the frontal cortex of rats with unilateral lesion to the NBM to find an appropriate index for the early detection of AD using techniques of nuclear medicine. The right NBM was injected with ibotenic acid. [18F]FDG-PET images were obtained 3 days later. Some rats were sacrificed at 1 week, whereas others were subjected to a second [18F]FDG-PET at 4 weeks then sacrificed for membrane preparation. The prepared membranes were subjected to radioreceptor assays to measure the density of nicotinic and muscarinic acetylcholine receptors. Glucose metabolism had decreased on the damaged side compared to the control side at 3 days, but at 4 weeks, there was no difference between the sides. Nicotinic acetylcholine receptors had significantly decreased in density compared to the control side at both 1 and 4 weeks. However, muscarinic receptors were not affected. These results suggested that neuronal dysfunction in AD could be diagnosed at an early stage by imaging nicotinic acetylcholine receptors.

Original languageEnglish
Pages (from-to)249-254
Number of pages6
JournalNuclear Medicine and Biology
Volume33
Issue number2
DOIs
Publication statusPublished - Feb 1 2006
Externally publishedYes

Keywords

  • Alzheimer's disease
  • Muscarinic acetylcholine receptor
  • NBM-lesioned rat
  • Nicotinic acetylcholine receptor
  • [F]FDG

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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