Change of central cholinergic receptors following lesions of nucleus basalis magnocellularis in rats: Search for an imaging index suitable for the early detection of Alzheimer's disease

Mikako Ogawa, Yasuhiko Iida, Masaki Nakagawa, Yugi Kuge, Hidekazu Kawashima, Akiko Tominaga, Masashi Ueda, Yasuhiro Magata, Hideo Saji

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Cholinergic system in the central nervous system is involved in the memory function. Thus, because the dysfunction of cholinergic system that project to the cerebral cortex from nucleus basalis of Meynert (nbM) would be implicated in the memory function deficits in Alzheimer's disease (AD), evaluating cholinergic function may be useful for the early detection of AD. In this study, because the nucleus basalis magnocellularis (NBM) in rats is equivalent to nbM in human, we investigated the change in cholinergic receptors in the frontal cortex of rats with unilateral lesion to the NBM to find an appropriate index for the early detection of AD using techniques of nuclear medicine. The right NBM was injected with ibotenic acid. [18F]FDG-PET images were obtained 3 days later. Some rats were sacrificed at 1 week, whereas others were subjected to a second [18F]FDG-PET at 4 weeks then sacrificed for membrane preparation. The prepared membranes were subjected to radioreceptor assays to measure the density of nicotinic and muscarinic acetylcholine receptors. Glucose metabolism had decreased on the damaged side compared to the control side at 3 days, but at 4 weeks, there was no difference between the sides. Nicotinic acetylcholine receptors had significantly decreased in density compared to the control side at both 1 and 4 weeks. However, muscarinic receptors were not affected. These results suggested that neuronal dysfunction in AD could be diagnosed at an early stage by imaging nicotinic acetylcholine receptors.

Original languageEnglish
Pages (from-to)249-254
Number of pages6
JournalNuclear Medicine and Biology
Volume33
Issue number2
DOIs
Publication statusPublished - Feb 2006
Externally publishedYes

Fingerprint

Basal Nucleus of Meynert
Cholinergic Receptors
Early Diagnosis
Alzheimer Disease
Nicotinic Receptors
Cholinergic Agents
Fluorodeoxyglucose F18
Muscarinic Receptors
Ibotenic Acid
Radioligand Assay
Membranes
Nuclear Medicine
Memory Disorders
Frontal Lobe
Cerebral Cortex
Central Nervous System
Glucose

Keywords

  • [F]FDG
  • Alzheimer's disease
  • Muscarinic acetylcholine receptor
  • NBM-lesioned rat
  • Nicotinic acetylcholine receptor

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging

Cite this

Change of central cholinergic receptors following lesions of nucleus basalis magnocellularis in rats : Search for an imaging index suitable for the early detection of Alzheimer's disease. / Ogawa, Mikako; Iida, Yasuhiko; Nakagawa, Masaki; Kuge, Yugi; Kawashima, Hidekazu; Tominaga, Akiko; Ueda, Masashi; Magata, Yasuhiro; Saji, Hideo.

In: Nuclear Medicine and Biology, Vol. 33, No. 2, 02.2006, p. 249-254.

Research output: Contribution to journalArticle

Ogawa, Mikako ; Iida, Yasuhiko ; Nakagawa, Masaki ; Kuge, Yugi ; Kawashima, Hidekazu ; Tominaga, Akiko ; Ueda, Masashi ; Magata, Yasuhiro ; Saji, Hideo. / Change of central cholinergic receptors following lesions of nucleus basalis magnocellularis in rats : Search for an imaging index suitable for the early detection of Alzheimer's disease. In: Nuclear Medicine and Biology. 2006 ; Vol. 33, No. 2. pp. 249-254.
@article{cf4681aba74244a596ffa6801beb9d76,
title = "Change of central cholinergic receptors following lesions of nucleus basalis magnocellularis in rats: Search for an imaging index suitable for the early detection of Alzheimer's disease",
abstract = "Cholinergic system in the central nervous system is involved in the memory function. Thus, because the dysfunction of cholinergic system that project to the cerebral cortex from nucleus basalis of Meynert (nbM) would be implicated in the memory function deficits in Alzheimer's disease (AD), evaluating cholinergic function may be useful for the early detection of AD. In this study, because the nucleus basalis magnocellularis (NBM) in rats is equivalent to nbM in human, we investigated the change in cholinergic receptors in the frontal cortex of rats with unilateral lesion to the NBM to find an appropriate index for the early detection of AD using techniques of nuclear medicine. The right NBM was injected with ibotenic acid. [18F]FDG-PET images were obtained 3 days later. Some rats were sacrificed at 1 week, whereas others were subjected to a second [18F]FDG-PET at 4 weeks then sacrificed for membrane preparation. The prepared membranes were subjected to radioreceptor assays to measure the density of nicotinic and muscarinic acetylcholine receptors. Glucose metabolism had decreased on the damaged side compared to the control side at 3 days, but at 4 weeks, there was no difference between the sides. Nicotinic acetylcholine receptors had significantly decreased in density compared to the control side at both 1 and 4 weeks. However, muscarinic receptors were not affected. These results suggested that neuronal dysfunction in AD could be diagnosed at an early stage by imaging nicotinic acetylcholine receptors.",
keywords = "[F]FDG, Alzheimer's disease, Muscarinic acetylcholine receptor, NBM-lesioned rat, Nicotinic acetylcholine receptor",
author = "Mikako Ogawa and Yasuhiko Iida and Masaki Nakagawa and Yugi Kuge and Hidekazu Kawashima and Akiko Tominaga and Masashi Ueda and Yasuhiro Magata and Hideo Saji",
year = "2006",
month = "2",
doi = "10.1002/cbic.200500397",
language = "English",
volume = "33",
pages = "249--254",
journal = "Nuclear Medicine and Biology",
issn = "0969-8051",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Change of central cholinergic receptors following lesions of nucleus basalis magnocellularis in rats

T2 - Search for an imaging index suitable for the early detection of Alzheimer's disease

AU - Ogawa, Mikako

AU - Iida, Yasuhiko

AU - Nakagawa, Masaki

AU - Kuge, Yugi

AU - Kawashima, Hidekazu

AU - Tominaga, Akiko

AU - Ueda, Masashi

AU - Magata, Yasuhiro

AU - Saji, Hideo

PY - 2006/2

Y1 - 2006/2

N2 - Cholinergic system in the central nervous system is involved in the memory function. Thus, because the dysfunction of cholinergic system that project to the cerebral cortex from nucleus basalis of Meynert (nbM) would be implicated in the memory function deficits in Alzheimer's disease (AD), evaluating cholinergic function may be useful for the early detection of AD. In this study, because the nucleus basalis magnocellularis (NBM) in rats is equivalent to nbM in human, we investigated the change in cholinergic receptors in the frontal cortex of rats with unilateral lesion to the NBM to find an appropriate index for the early detection of AD using techniques of nuclear medicine. The right NBM was injected with ibotenic acid. [18F]FDG-PET images were obtained 3 days later. Some rats were sacrificed at 1 week, whereas others were subjected to a second [18F]FDG-PET at 4 weeks then sacrificed for membrane preparation. The prepared membranes were subjected to radioreceptor assays to measure the density of nicotinic and muscarinic acetylcholine receptors. Glucose metabolism had decreased on the damaged side compared to the control side at 3 days, but at 4 weeks, there was no difference between the sides. Nicotinic acetylcholine receptors had significantly decreased in density compared to the control side at both 1 and 4 weeks. However, muscarinic receptors were not affected. These results suggested that neuronal dysfunction in AD could be diagnosed at an early stage by imaging nicotinic acetylcholine receptors.

AB - Cholinergic system in the central nervous system is involved in the memory function. Thus, because the dysfunction of cholinergic system that project to the cerebral cortex from nucleus basalis of Meynert (nbM) would be implicated in the memory function deficits in Alzheimer's disease (AD), evaluating cholinergic function may be useful for the early detection of AD. In this study, because the nucleus basalis magnocellularis (NBM) in rats is equivalent to nbM in human, we investigated the change in cholinergic receptors in the frontal cortex of rats with unilateral lesion to the NBM to find an appropriate index for the early detection of AD using techniques of nuclear medicine. The right NBM was injected with ibotenic acid. [18F]FDG-PET images were obtained 3 days later. Some rats were sacrificed at 1 week, whereas others were subjected to a second [18F]FDG-PET at 4 weeks then sacrificed for membrane preparation. The prepared membranes were subjected to radioreceptor assays to measure the density of nicotinic and muscarinic acetylcholine receptors. Glucose metabolism had decreased on the damaged side compared to the control side at 3 days, but at 4 weeks, there was no difference between the sides. Nicotinic acetylcholine receptors had significantly decreased in density compared to the control side at both 1 and 4 weeks. However, muscarinic receptors were not affected. These results suggested that neuronal dysfunction in AD could be diagnosed at an early stage by imaging nicotinic acetylcholine receptors.

KW - [F]FDG

KW - Alzheimer's disease

KW - Muscarinic acetylcholine receptor

KW - NBM-lesioned rat

KW - Nicotinic acetylcholine receptor

UR - http://www.scopus.com/inward/record.url?scp=33644942324&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644942324&partnerID=8YFLogxK

U2 - 10.1002/cbic.200500397

DO - 10.1002/cbic.200500397

M3 - Article

C2 - 16546680

AN - SCOPUS:33644942324

VL - 33

SP - 249

EP - 254

JO - Nuclear Medicine and Biology

JF - Nuclear Medicine and Biology

SN - 0969-8051

IS - 2

ER -