Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L.) with age and/or role

Takeo Kubo, Masami Sasaki, Jun Nakamura, Hiromi Sasagawa, Kazuaki Ohashi, Hideaki Takeuchi, Shunji Natori

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

The roles of adult worker honeybees change with age; young workers nurse brood by secreting bee milk (royal jelly), and older workers forage for nectar and pollen and process nectar into honey. The electrophoretic profile of worker hypopharyngeal-gland proteins changes with age and/or role. Immunoblotting analysis using affinity-purified antibodies against three major proteins (50, 56, and 64 kDa) of the nurse-bee gland showed that they are synthesized selectively and secreted as bee-milk proteins. Immunofluorescence study showed that the proteins condense in the duct after secretion from acini. However, a major 70-kDa protein synthesized specifically in the forager-bee hypopharyngeal gland was identified as an α-glucosidase. Therefore, the hypopharyngeal gland seems to have two distinct states differentiated by synthesizing of different major proteins depending on the age-dependent role change.

Original languageEnglish
Pages (from-to)291-295
Number of pages5
JournalJournal of biochemistry
Volume119
Issue number2
DOIs
Publication statusPublished - Feb 1996
Externally publishedYes

Keywords

  • Honeybee
  • Hypopharyngeal gland
  • Role change
  • Royal jelly
  • α-glucosidase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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