Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L.) with age and/or role

Takeo Kubo, Masami Sasaki, Jun Nakamura, Hiromi Sasagawa, Kazuaki Ohashi, Hideaki Takeuchi, Shunji Natori

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

The roles of adult worker honeybees change with age; young workers nurse brood by secreting bee milk (royal jelly), and older workers forage for nectar and pollen and process nectar into honey. The electrophoretic profile of worker hypopharyngeal-gland proteins changes with age and/or role. Immunoblotting analysis using affinity-purified antibodies against three major proteins (50, 56, and 64 kDa) of the nurse-bee gland showed that they are synthesized selectively and secreted as bee-milk proteins. Immunofluorescence study showed that the proteins condense in the duct after secretion from acini. However, a major 70-kDa protein synthesized specifically in the forager-bee hypopharyngeal gland was identified as an α-glucosidase. Therefore, the hypopharyngeal gland seems to have two distinct states differentiated by synthesizing of different major proteins depending on the age-dependent role change.

Original languageEnglish
Pages (from-to)291-295
Number of pages5
JournalJournal of Biochemistry
Volume119
Issue number2
Publication statusPublished - Feb 1996
Externally publishedYes

Fingerprint

Bees
Plant Nectar
Proteins
Nurses
Glucosidases
Antibody Affinity
Honey
Milk Proteins
Pollen
Immunoblotting
Ducts
Fluorescent Antibody Technique
Milk
Antibodies

Keywords

  • α-glucosidase
  • Honeybee
  • Hypopharyngeal gland
  • Role change
  • Royal jelly

ASJC Scopus subject areas

  • Biochemistry

Cite this

Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L.) with age and/or role. / Kubo, Takeo; Sasaki, Masami; Nakamura, Jun; Sasagawa, Hiromi; Ohashi, Kazuaki; Takeuchi, Hideaki; Natori, Shunji.

In: Journal of Biochemistry, Vol. 119, No. 2, 02.1996, p. 291-295.

Research output: Contribution to journalArticle

Kubo, T, Sasaki, M, Nakamura, J, Sasagawa, H, Ohashi, K, Takeuchi, H & Natori, S 1996, 'Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L.) with age and/or role', Journal of Biochemistry, vol. 119, no. 2, pp. 291-295.
Kubo, Takeo ; Sasaki, Masami ; Nakamura, Jun ; Sasagawa, Hiromi ; Ohashi, Kazuaki ; Takeuchi, Hideaki ; Natori, Shunji. / Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L.) with age and/or role. In: Journal of Biochemistry. 1996 ; Vol. 119, No. 2. pp. 291-295.
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