Cessation of Fgf10 signaling, resulting in a defective dental epithelial stem cell compartment, leads to the transition from crown to root formation

Tamaki Yokohama-Tamaki, Hayato Ohshima, Naoki Fujiwara, Yunosuke Takada, Yasuo Ichimori, Satoshi Waklsaka, Hideyo Ohuchi, Hidemitsu Harada

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Mouse, rat and human molars begin to form root after the completion of crown formation. In these teeth, fibroblast growth factor (Fgf) 10 disappears in the transitional stage from crown formation to root. By contrast, rodent incisors and vole molars demonstrate continuous growth, owing to the formation and maintenance of a stem cell compartment by the constant expression of Fgf10. To clarify the relationship between root formation and disappearance of Fgf10, we carried out two experiments for the loss and gain of Fgf10 function. First, we examined postnatal growth in the incisors of Fgf10-deficient mice, which have the defect of a dental epithelial stem cell compartment referred to as 'apical bud', after implantation under the kidney capsule. The growth at the labial side in the mutant mice mimics the development of limited-growth teeth. 5′-Bromo-2′-deoxyuridine (BrdU) labeling and cytokeratin (CK) 14 and Notch2 immunostaining suggested that the inhibition of inner enamel epithelium growth and the more-active proliferation of the outer enamel epithelium and/or stellate reticulum result in Hertwig's epithelial root sheath formation. Second, we examined the effects of Fgf10 overexpression in the transitional stage of molar germs, which led to the formation of apical bud involving in the inhibition of HERS formation. Taken together, these results suggest that the disappearance of Fgf10 signaling leads to the transition from crown to root formation, owing to the loss of a dental epithelial stem cell compartment.

Original languageEnglish
Pages (from-to)1359-1366
Number of pages8
JournalDevelopment
Volume133
Issue number7
DOIs
Publication statusPublished - Apr 2006
Externally publishedYes

Fingerprint

Crowns
Tooth
Stem Cells
Epithelial Cells
Incisor
Dental Enamel
Growth
Epithelium
Fibroblast Growth Factor 10
Keratin-14
Reticulum
Arvicolinae
Bromodeoxyuridine
Lip
Growth and Development
Capsules
Rodentia
Maintenance
Kidney

Keywords

  • Apical bud
  • Fibroblast growth factor 10
  • Hertwig's epithelial roots sheath (HERS)
  • Inner enamel epithelium
  • Outer enamel epithelium

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Yokohama-Tamaki, T., Ohshima, H., Fujiwara, N., Takada, Y., Ichimori, Y., Waklsaka, S., ... Harada, H. (2006). Cessation of Fgf10 signaling, resulting in a defective dental epithelial stem cell compartment, leads to the transition from crown to root formation. Development, 133(7), 1359-1366. https://doi.org/10.1242/dev.02307

Cessation of Fgf10 signaling, resulting in a defective dental epithelial stem cell compartment, leads to the transition from crown to root formation. / Yokohama-Tamaki, Tamaki; Ohshima, Hayato; Fujiwara, Naoki; Takada, Yunosuke; Ichimori, Yasuo; Waklsaka, Satoshi; Ohuchi, Hideyo; Harada, Hidemitsu.

In: Development, Vol. 133, No. 7, 04.2006, p. 1359-1366.

Research output: Contribution to journalArticle

Yokohama-Tamaki, T, Ohshima, H, Fujiwara, N, Takada, Y, Ichimori, Y, Waklsaka, S, Ohuchi, H & Harada, H 2006, 'Cessation of Fgf10 signaling, resulting in a defective dental epithelial stem cell compartment, leads to the transition from crown to root formation', Development, vol. 133, no. 7, pp. 1359-1366. https://doi.org/10.1242/dev.02307
Yokohama-Tamaki, Tamaki ; Ohshima, Hayato ; Fujiwara, Naoki ; Takada, Yunosuke ; Ichimori, Yasuo ; Waklsaka, Satoshi ; Ohuchi, Hideyo ; Harada, Hidemitsu. / Cessation of Fgf10 signaling, resulting in a defective dental epithelial stem cell compartment, leads to the transition from crown to root formation. In: Development. 2006 ; Vol. 133, No. 7. pp. 1359-1366.
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