Cerasome as an infusible, cell-friendly, and serum-compatible transfection agent in a viral size

Kazuki Matsui, Shinsuke Sando, Takashi Sera, Yasuhiro Aoyama, Yoshihiro Sasaki, Takayoshi Komatsu, Takashi Terashima, Jun Ichi Kikuchi

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Alanine-based cationic lipid 1 having a (EtO)3SiCH2CH2CH2 group on the quaternized ammonium nitrogen forms a liposome which self-rigidifies via in situ sol-gel processes (Si-OEt + H2O → Si-OH + EtOH followed by 2Si-OH → Si-O-Si + H2O) on the surface. The resulting cerasome (partially ceramic- or silica-coated liposome) (60-70 nm) retains the integrity of such in the complexation with lucifarase-encoding plasmid DNA pGL3. The resultant pGL3 complex of infusible or monomeric cerasome in a viral size (∼70 nm) exhibits a remarkable transfection performance toward HeLa and HepG2 cells with a 102-3-fold higher efficiency (relative to that of the nonsilylated reference lipid 2), minimized cytotoxicity, and serum compatibility. Reference lipid 2, i.e., alanine-based lipid having a simple quaternized ammonium headgroup, forms liposome (60-70 nm) which is less self-confined and more mobile undergoes DNA-induced fusion to give endocytosis-irrelevant and more toxic bigger (100-300 nm) particles. The silicon strategy thus provides a simple and widely applicable tool to overcome general problems associated with current technology of artificial gene delivery.

Original languageEnglish
Pages (from-to)3114-3115
Number of pages2
JournalJournal of the American Chemical Society
Volume128
Issue number10
DOIs
Publication statusPublished - Mar 15 2006
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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  • Cite this

    Matsui, K., Sando, S., Sera, T., Aoyama, Y., Sasaki, Y., Komatsu, T., Terashima, T., & Kikuchi, J. I. (2006). Cerasome as an infusible, cell-friendly, and serum-compatible transfection agent in a viral size. Journal of the American Chemical Society, 128(10), 3114-3115. https://doi.org/10.1021/ja058016i