Cerasome as an infusible, cell-friendly, and serum-compatible transfection agent in a viral size

Kazuki Matsui, Shinsuke Sando, Takashi Sera, Yasuhiro Aoyama, Yoshihiro Sasaki, Takayoshi Komatsu, Takashi Terashima, Jun Ichi Kikuchi

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Alanine-based cationic lipid 1 having a (EtO)3SiCH2CH2CH2 group on the quaternized ammonium nitrogen forms a liposome which self-rigidifies via in situ sol-gel processes (Si-OEt + H2O → Si-OH + EtOH followed by 2Si-OH → Si-O-Si + H2O) on the surface. The resulting cerasome (partially ceramic- or silica-coated liposome) (60-70 nm) retains the integrity of such in the complexation with lucifarase-encoding plasmid DNA pGL3. The resultant pGL3 complex of infusible or monomeric cerasome in a viral size (∼70 nm) exhibits a remarkable transfection performance toward HeLa and HepG2 cells with a 102-3-fold higher efficiency (relative to that of the nonsilylated reference lipid 2), minimized cytotoxicity, and serum compatibility. Reference lipid 2, i.e., alanine-based lipid having a simple quaternized ammonium headgroup, forms liposome (60-70 nm) which is less self-confined and more mobile undergoes DNA-induced fusion to give endocytosis-irrelevant and more toxic bigger (100-300 nm) particles. The silicon strategy thus provides a simple and widely applicable tool to overcome general problems associated with current technology of artificial gene delivery.

Original languageEnglish
Pages (from-to)3114-3115
Number of pages2
JournalJournal of the American Chemical Society
Volume128
Issue number10
DOIs
Publication statusPublished - Mar 15 2006
Externally publishedYes

Fingerprint

Lipids
Transfection
Liposomes
Serum
Ammonium Compounds
Alanine
DNA
Synthetic Genes
Poisons
Hep G2 Cells
Ceramics
Polymethyl Methacrylate
Silicon
Cytotoxicity
Endocytosis
Complexation
HeLa Cells
Silicon Dioxide
Sol-gel process
Plasmids

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Cerasome as an infusible, cell-friendly, and serum-compatible transfection agent in a viral size. / Matsui, Kazuki; Sando, Shinsuke; Sera, Takashi; Aoyama, Yasuhiro; Sasaki, Yoshihiro; Komatsu, Takayoshi; Terashima, Takashi; Kikuchi, Jun Ichi.

In: Journal of the American Chemical Society, Vol. 128, No. 10, 15.03.2006, p. 3114-3115.

Research output: Contribution to journalArticle

Matsui, K, Sando, S, Sera, T, Aoyama, Y, Sasaki, Y, Komatsu, T, Terashima, T & Kikuchi, JI 2006, 'Cerasome as an infusible, cell-friendly, and serum-compatible transfection agent in a viral size', Journal of the American Chemical Society, vol. 128, no. 10, pp. 3114-3115. https://doi.org/10.1021/ja058016i
Matsui, Kazuki ; Sando, Shinsuke ; Sera, Takashi ; Aoyama, Yasuhiro ; Sasaki, Yoshihiro ; Komatsu, Takayoshi ; Terashima, Takashi ; Kikuchi, Jun Ichi. / Cerasome as an infusible, cell-friendly, and serum-compatible transfection agent in a viral size. In: Journal of the American Chemical Society. 2006 ; Vol. 128, No. 10. pp. 3114-3115.
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