Cepharanthin enhances thermosensitivity without a resultant reduction in the thermotolerance of a murine mammary carcinoma

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Abstract

Cepharanthin (Ce) is a biscoclaurine alkaloid extracted from Steplmnia cepharantha Hayata. The results of our previous in vitro study indicated that Ce reduces thermotolerance by enhancing thermosensitivity. In the present study, we investigated the in vitro and in vivo effects of Ce on thermosensitivity and thermotolerance using a murine mammary carcinoma, MCa, and C3H/HeN mice. Ce enhanced the thermosensitivity of MCa cells for heating at 44°C not only in vitro but also in vivo. The in vivo enhancement ratio ± SD of Ce at 100 mg/kg for heating at 44°C was 1.3±0.3. The fractionated heat treatments at 44°C for 30 and 60 min with an interval time of 0-6 days resulted in the development of remarkable thermotolerance and the expression of heat shock protein 70 in MCa tumors after the first heating. Ce at 100 mg/ kg given immediately after the first heating increased the expression of heat shock protein 70 in MCa tumors, and did not reduce the development of thermotolerance. Ce given immediately before the first or second heating also did not inhibit the thermotolerance. The results of this study suggest that Ce enhances the thermosensitivity of MCa tumors as a thermosensitizer, but that this mild thermosensitizing property of Ce might be insufficient to conquer the remarkable thermotolerance in MCa tumors that develops after the first heating.

Original languageEnglish
Pages (from-to)95-99
Number of pages5
JournalInternational Journal of Oncology
Volume15
Issue number1
Publication statusPublished - 1999

Fingerprint

Breast Neoplasms
Heating
HSP70 Heat-Shock Proteins
Neoplasms
Thermotolerance
cepharanthine
Inbred C3H Mouse
Alkaloids
Hot Temperature
In Vitro Techniques

Keywords

  • Cepharanthin
  • Hyperthermia
  • Mammary carcinoma
  • Thermosensitivity
  • Thermotolerancc

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

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abstract = "Cepharanthin (Ce) is a biscoclaurine alkaloid extracted from Steplmnia cepharantha Hayata. The results of our previous in vitro study indicated that Ce reduces thermotolerance by enhancing thermosensitivity. In the present study, we investigated the in vitro and in vivo effects of Ce on thermosensitivity and thermotolerance using a murine mammary carcinoma, MCa, and C3H/HeN mice. Ce enhanced the thermosensitivity of MCa cells for heating at 44°C not only in vitro but also in vivo. The in vivo enhancement ratio ± SD of Ce at 100 mg/kg for heating at 44°C was 1.3±0.3. The fractionated heat treatments at 44°C for 30 and 60 min with an interval time of 0-6 days resulted in the development of remarkable thermotolerance and the expression of heat shock protein 70 in MCa tumors after the first heating. Ce at 100 mg/ kg given immediately after the first heating increased the expression of heat shock protein 70 in MCa tumors, and did not reduce the development of thermotolerance. Ce given immediately before the first or second heating also did not inhibit the thermotolerance. The results of this study suggest that Ce enhances the thermosensitivity of MCa tumors as a thermosensitizer, but that this mild thermosensitizing property of Ce might be insufficient to conquer the remarkable thermotolerance in MCa tumors that develops after the first heating.",
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AB - Cepharanthin (Ce) is a biscoclaurine alkaloid extracted from Steplmnia cepharantha Hayata. The results of our previous in vitro study indicated that Ce reduces thermotolerance by enhancing thermosensitivity. In the present study, we investigated the in vitro and in vivo effects of Ce on thermosensitivity and thermotolerance using a murine mammary carcinoma, MCa, and C3H/HeN mice. Ce enhanced the thermosensitivity of MCa cells for heating at 44°C not only in vitro but also in vivo. The in vivo enhancement ratio ± SD of Ce at 100 mg/kg for heating at 44°C was 1.3±0.3. The fractionated heat treatments at 44°C for 30 and 60 min with an interval time of 0-6 days resulted in the development of remarkable thermotolerance and the expression of heat shock protein 70 in MCa tumors after the first heating. Ce at 100 mg/ kg given immediately after the first heating increased the expression of heat shock protein 70 in MCa tumors, and did not reduce the development of thermotolerance. Ce given immediately before the first or second heating also did not inhibit the thermotolerance. The results of this study suggest that Ce enhances the thermosensitivity of MCa tumors as a thermosensitizer, but that this mild thermosensitizing property of Ce might be insufficient to conquer the remarkable thermotolerance in MCa tumors that develops after the first heating.

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