Centromeric repetitive sequences in Arabidopsis thaliana

M. Murata, Y. Ogura, F. Motoyoshi

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Two highly repetitive DNA sequences have been cloned from Arabidopsis thaliana, ecotype Columbia, and were characterized by molecular and cytological analyses. These two sequences belong to the same repeat family with 180-bp basic unit, being tandemly organized in clusters. Pulsed field gel electrophoresis showed that this repeat sequence family forms at least seven clusters from ca. 100 to 1200 kb in length and ca. 3500 kb in total. Fluorescent in situ hybridization to somatic metaphase cells with the monomeric repeat unit as a probe clearly revealed that this repeat family is located at the centromeric regions of all chromosomes. It was also shown that this repetitive sequence is closely associated with limited parts of heterochromatic blocks on the centromeric regions which are visible distinctly at meiotic prophase from leptotene to diakinesis. Furthermore, this sequence hybridized preferentially to both polar sides of five bivalent chromosomes at the first metaphase. These results suggest that the repetitive sequences of this family were derived from the regions very close to the centromeres or on the centromeres themselves.

Original languageEnglish
Pages (from-to)361-370
Number of pages10
JournalJapanese Journal of Genetics
Volume69
Issue number4
Publication statusPublished - 1994

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Nucleic Acid Repetitive Sequences
Arabidopsis
Centromere
Metaphase
Chromosomes
Meiotic Prophase I
Ecotype
Prophase
Pulsed Field Gel Electrophoresis
Fluorescence In Situ Hybridization

ASJC Scopus subject areas

  • Genetics

Cite this

Murata, M., Ogura, Y., & Motoyoshi, F. (1994). Centromeric repetitive sequences in Arabidopsis thaliana. Japanese Journal of Genetics, 69(4), 361-370.

Centromeric repetitive sequences in Arabidopsis thaliana. / Murata, M.; Ogura, Y.; Motoyoshi, F.

In: Japanese Journal of Genetics, Vol. 69, No. 4, 1994, p. 361-370.

Research output: Contribution to journalArticle

Murata, M, Ogura, Y & Motoyoshi, F 1994, 'Centromeric repetitive sequences in Arabidopsis thaliana', Japanese Journal of Genetics, vol. 69, no. 4, pp. 361-370.
Murata, M. ; Ogura, Y. ; Motoyoshi, F. / Centromeric repetitive sequences in Arabidopsis thaliana. In: Japanese Journal of Genetics. 1994 ; Vol. 69, No. 4. pp. 361-370.
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