TY - JOUR
T1 - Centromeres of Cucumis melo L. Comprise Cmcent and two novel repeats, CmSat162 and CmSat189
AU - Setiawan, Agus Budi
AU - Teo, Chee How
AU - Kikuchi, Shinji
AU - Sassa, Hidenori
AU - Kato, Kenji
AU - Koba, Takato
N1 - Funding Information:
This work was financially supported in part by Japan Society for Promotion of Science (JSPS) KAKENHI Grant Number JP 16K07588 to TK. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We would like to thank Henda Harmantia Dewi for editing and correction of this manuscript and Kousuke Kuroda for laboratory technical assistance.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Centromeres are prerequisite for accurate segregation and are landmarks of primary constrictions of metaphase chromosomes in eukaryotes. In melon, high-copy-number satellite DNAs (SatDNAs) were found at various chromosomal locations such as centromeric, pericentromeric, and subtelomeric regions. In the present study, utilizing the published draft genome sequence of melon, two new SatDNAs (CmSat162 and CmSat189) of melon were identified and their chromosomal distributions were confirmed using fluorescence in situ hybridization. DNA probes prepared from these SatDNAs were successfully hybridized to melon somatic and meiotic chromosomes. CmSat162 was located on 12 pairs of melon chromosomes and co-localized with the centromeric repeat, Cmcent, at the centromeric regions. In contrast, CmSat189 was found to be located not only on centromeric regions but also on specific regions of the chromosomes, allowing the characterization of individual chromosomes of melon. It was also shown that these SatDNAs were transcribed in melon. These results suggest that CmSat162 and CmSat189 might have some functions at the centromeric regions.
AB - Centromeres are prerequisite for accurate segregation and are landmarks of primary constrictions of metaphase chromosomes in eukaryotes. In melon, high-copy-number satellite DNAs (SatDNAs) were found at various chromosomal locations such as centromeric, pericentromeric, and subtelomeric regions. In the present study, utilizing the published draft genome sequence of melon, two new SatDNAs (CmSat162 and CmSat189) of melon were identified and their chromosomal distributions were confirmed using fluorescence in situ hybridization. DNA probes prepared from these SatDNAs were successfully hybridized to melon somatic and meiotic chromosomes. CmSat162 was located on 12 pairs of melon chromosomes and co-localized with the centromeric repeat, Cmcent, at the centromeric regions. In contrast, CmSat189 was found to be located not only on centromeric regions but also on specific regions of the chromosomes, allowing the characterization of individual chromosomes of melon. It was also shown that these SatDNAs were transcribed in melon. These results suggest that CmSat162 and CmSat189 might have some functions at the centromeric regions.
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U2 - 10.1371/journal.pone.0227578
DO - 10.1371/journal.pone.0227578
M3 - Article
C2 - 31945109
AN - SCOPUS:85078031010
VL - 15
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 1
M1 - e0227578
ER -