Central neuropeptide Y induces proximal stomach relaxation via Y1 receptors in the dorsal vagal complex of the rat

Motoi Kobashi, Yuichi Shimatani, Keisuke Shirota, Song Yu Xuan, Yoshihiro Mitoh, Ryuji Matsuo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Effects of neuropeptide Y (NPY) on motility of the proximal stomach was examined in anesthetized rats. Intragastric pressure was measured using a balloon situated in the proximal part of the stomach. Administration of NPY into the fourth ventricle induced relaxation of the proximal stomach in a dose-dependent manner. Administration of an Y1 receptor (Y1R) agonist [Leu 31, Pro34]NPY induced a larger relaxation than NPY. The administration of an Y2 receptor agonist (NPY 13-36) did not induce significant changes in motility. Microinjections of [Leu31, Pro34]NPY into the caudal part of the dorsal vagal complex (DVC) induced relaxation of the proximal stomach. In contrast, similar injections into the intermediate part of the DVC increased IGP of the proximal stomach. Administration of NPY into the fourth ventricle did not induce relaxation after bilateral injections of the Y1R antagonist (1229U91) into the caudal DVC. These results indicate that NPY induces relaxation in the proximal stomach via Y1Rs situated in the DVC. Because bilateral vagotomy below the diaphragm abolished the relaxation induced by the administration of NPY into the fourth ventricle, relaxation induced by NPY is probably mediated by vagal preganglionic neurons. Intravenous injection of atropine methyl nitrate reduced relaxation induced by administration of NPY. Therefore, relaxation induced by NPY is likely mediated by peripheral cholinergic neurons.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume290
Issue number2
DOIs
Publication statusPublished - Feb 2006

Fingerprint

Neuropeptide Y
Stomach
Fourth Ventricle
Injections
Cholinergic Neurons
Vagotomy
Microinjections
Diaphragm
Atropine
Intravenous Injections
Neurons
Pressure

Keywords

  • Feeding
  • Fundus
  • Gastric
  • Medulla
  • Vagus

ASJC Scopus subject areas

  • Physiology

Cite this

Central neuropeptide Y induces proximal stomach relaxation via Y1 receptors in the dorsal vagal complex of the rat. / Kobashi, Motoi; Shimatani, Yuichi; Shirota, Keisuke; Xuan, Song Yu; Mitoh, Yoshihiro; Matsuo, Ryuji.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 290, No. 2, 02.2006.

Research output: Contribution to journalArticle

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