Abstract
Angiotensin II (Ang II) stimulates oral water intake by causing thirst in all terrestrial vertebrates except anurans. Anuran amphibians do not drink orally but absorb water osmotically through ventral skin. In this study, we examined the role of Ang II on the regulation of water-absorption behavior in the Japanese tree frog (Hyla japonica). In fully hydrated frogs, intracerebroventricular (ICV) and intralymphatic sac (ILS) injection of Ang II significantly extended the residence time of water in a dose-dependent manner. Ang II-dependent water uptake was inhibited by ICV pretreatment with an angiotensin II type-1 (AT1) receptor antagonist but not a type-2 (AT2) receptor antagonist. These results suggest that Ang II stimulates water-absorption behavior in the tree frog via an AT1-like but not AT2-like receptor. We then cloned and characterized cDNA of the tree frog AT1 receptor from the brain. The tree frog AT1 receptor cDNA encodes a 361 amino acid residue protein, which is 87% identical to the toad (Bufo marinus) AT1 receptor and exhibits the functional characteristics of an Ang II receptor. AT1 receptor mRNAs were found to be present in a number of tissues including brain (especially in the diencephalon), lung, large intestine, kidney and ventral pelvic skin. When tree frogs were exposed to dehydrating conditions, AT1 receptor mRNA significantly increased in the diencephalon and the rhombencephalon. These data suggest that central Ang II may control water intake behavior via an AT1 receptor on the diencephalon and rhombencephalon in anuran amphibians and may have implications for water consumption in vertebrates.
| Original language | English |
|---|---|
| Pages (from-to) | 457-464 |
| Number of pages | 8 |
| Journal | Hormones and Behavior |
| Volume | 58 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Aug 1 2010 |
| Externally published | Yes |
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Keywords
- Ang II
- Angiotensin type-1 receptor
- Anuran
- Candesartan
- Osmoregulation
- Tree frog
- Water-absorption behavior
ASJC Scopus subject areas
- Endocrinology
- Endocrine and Autonomic Systems
- Behavioral Neuroscience
Cite this
Central angiotensin II stimulates cutaneous water intake behavior via an angiotensin II type-1 receptor pathway in the Japanese tree frog Hyla japonica. / Maejima, Sho; Konno, Norifumi; Matsuda, Kouhei; Uchiyama, Minoru.
In: Hormones and Behavior, Vol. 58, No. 3, 01.08.2010, p. 457-464.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Central angiotensin II stimulates cutaneous water intake behavior via an angiotensin II type-1 receptor pathway in the Japanese tree frog Hyla japonica
AU - Maejima, Sho
AU - Konno, Norifumi
AU - Matsuda, Kouhei
AU - Uchiyama, Minoru
PY - 2010/8/1
Y1 - 2010/8/1
N2 - Angiotensin II (Ang II) stimulates oral water intake by causing thirst in all terrestrial vertebrates except anurans. Anuran amphibians do not drink orally but absorb water osmotically through ventral skin. In this study, we examined the role of Ang II on the regulation of water-absorption behavior in the Japanese tree frog (Hyla japonica). In fully hydrated frogs, intracerebroventricular (ICV) and intralymphatic sac (ILS) injection of Ang II significantly extended the residence time of water in a dose-dependent manner. Ang II-dependent water uptake was inhibited by ICV pretreatment with an angiotensin II type-1 (AT1) receptor antagonist but not a type-2 (AT2) receptor antagonist. These results suggest that Ang II stimulates water-absorption behavior in the tree frog via an AT1-like but not AT2-like receptor. We then cloned and characterized cDNA of the tree frog AT1 receptor from the brain. The tree frog AT1 receptor cDNA encodes a 361 amino acid residue protein, which is 87% identical to the toad (Bufo marinus) AT1 receptor and exhibits the functional characteristics of an Ang II receptor. AT1 receptor mRNAs were found to be present in a number of tissues including brain (especially in the diencephalon), lung, large intestine, kidney and ventral pelvic skin. When tree frogs were exposed to dehydrating conditions, AT1 receptor mRNA significantly increased in the diencephalon and the rhombencephalon. These data suggest that central Ang II may control water intake behavior via an AT1 receptor on the diencephalon and rhombencephalon in anuran amphibians and may have implications for water consumption in vertebrates.
AB - Angiotensin II (Ang II) stimulates oral water intake by causing thirst in all terrestrial vertebrates except anurans. Anuran amphibians do not drink orally but absorb water osmotically through ventral skin. In this study, we examined the role of Ang II on the regulation of water-absorption behavior in the Japanese tree frog (Hyla japonica). In fully hydrated frogs, intracerebroventricular (ICV) and intralymphatic sac (ILS) injection of Ang II significantly extended the residence time of water in a dose-dependent manner. Ang II-dependent water uptake was inhibited by ICV pretreatment with an angiotensin II type-1 (AT1) receptor antagonist but not a type-2 (AT2) receptor antagonist. These results suggest that Ang II stimulates water-absorption behavior in the tree frog via an AT1-like but not AT2-like receptor. We then cloned and characterized cDNA of the tree frog AT1 receptor from the brain. The tree frog AT1 receptor cDNA encodes a 361 amino acid residue protein, which is 87% identical to the toad (Bufo marinus) AT1 receptor and exhibits the functional characteristics of an Ang II receptor. AT1 receptor mRNAs were found to be present in a number of tissues including brain (especially in the diencephalon), lung, large intestine, kidney and ventral pelvic skin. When tree frogs were exposed to dehydrating conditions, AT1 receptor mRNA significantly increased in the diencephalon and the rhombencephalon. These data suggest that central Ang II may control water intake behavior via an AT1 receptor on the diencephalon and rhombencephalon in anuran amphibians and may have implications for water consumption in vertebrates.
KW - Ang II
KW - Angiotensin type-1 receptor
KW - Anuran
KW - Candesartan
KW - Osmoregulation
KW - Tree frog
KW - Water-absorption behavior
UR - http://www.scopus.com/inward/record.url?scp=77955272920&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955272920&partnerID=8YFLogxK
U2 - 10.1016/j.yhbeh.2010.05.007
DO - 10.1016/j.yhbeh.2010.05.007
M3 - Article
C2 - 20483358
AN - SCOPUS:77955272920
VL - 58
SP - 457
EP - 464
JO - Hormones and Behavior
JF - Hormones and Behavior
SN - 0018-506X
IS - 3
ER -