Abstract
The chromosomal location of centromere-specific histone H3 (CENH3) is the assembly site for the kinetochore complex of active centromeres. Chromatin immunoprecipitation data indicated that CENH3 interacts in barley with cereba, a centromeric retroelement (CR)-like element conserved among cereal centromeres and barley-specific GC-rich centromeric satellite sequences. Anti-CENH3 signals on extended chromatin fibers always colocalized with the centromeric sequences but did not encompass the entire area covered by such centromeric repeats. This indicates that the CENH3 protein is bound only to a fraction of the centromeric repeats. At mitotic metaphase, CENH3, histone H3, and serine 10 phosphorylated histone H3 predominated within distinct structural subdomains of the centromere, as demonstrated by immunogold labeling for high resolution scanning electron microscopy.
| Original language | English |
|---|---|
| Pages (from-to) | 275-283 |
| Number of pages | 9 |
| Journal | Chromosoma |
| Volume | 116 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jun 2007 |
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- Genetics
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CENH3 interacts with the centromeric retrotransposon cereba and GC-rich satellites and locates to centromeric substructures in barley. / Houben, Andreas; Schroeder-Reiter, Elizabeth; Nagaki, Kiyotaka; Nasuda, Shuhei; Wanner, Gerhard; Murata, Minoru; Endo, Takashi R.
In: Chromosoma, Vol. 116, No. 3, 06.2007, p. 275-283.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CENH3 interacts with the centromeric retrotransposon cereba and GC-rich satellites and locates to centromeric substructures in barley
AU - Houben, Andreas
AU - Schroeder-Reiter, Elizabeth
AU - Nagaki, Kiyotaka
AU - Nasuda, Shuhei
AU - Wanner, Gerhard
AU - Murata, Minoru
AU - Endo, Takashi R.
PY - 2007/6
Y1 - 2007/6
N2 - The chromosomal location of centromere-specific histone H3 (CENH3) is the assembly site for the kinetochore complex of active centromeres. Chromatin immunoprecipitation data indicated that CENH3 interacts in barley with cereba, a centromeric retroelement (CR)-like element conserved among cereal centromeres and barley-specific GC-rich centromeric satellite sequences. Anti-CENH3 signals on extended chromatin fibers always colocalized with the centromeric sequences but did not encompass the entire area covered by such centromeric repeats. This indicates that the CENH3 protein is bound only to a fraction of the centromeric repeats. At mitotic metaphase, CENH3, histone H3, and serine 10 phosphorylated histone H3 predominated within distinct structural subdomains of the centromere, as demonstrated by immunogold labeling for high resolution scanning electron microscopy.
AB - The chromosomal location of centromere-specific histone H3 (CENH3) is the assembly site for the kinetochore complex of active centromeres. Chromatin immunoprecipitation data indicated that CENH3 interacts in barley with cereba, a centromeric retroelement (CR)-like element conserved among cereal centromeres and barley-specific GC-rich centromeric satellite sequences. Anti-CENH3 signals on extended chromatin fibers always colocalized with the centromeric sequences but did not encompass the entire area covered by such centromeric repeats. This indicates that the CENH3 protein is bound only to a fraction of the centromeric repeats. At mitotic metaphase, CENH3, histone H3, and serine 10 phosphorylated histone H3 predominated within distinct structural subdomains of the centromere, as demonstrated by immunogold labeling for high resolution scanning electron microscopy.
UR - http://www.scopus.com/inward/record.url?scp=34248157732&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34248157732&partnerID=8YFLogxK
U2 - 10.1007/s00412-007-0102-z
DO - 10.1007/s00412-007-0102-z
M3 - Article
C2 - 17483978
AN - SCOPUS:34248157732
VL - 116
SP - 275
EP - 283
JO - Chromosoma
JF - Chromosoma
SN - 0009-5915
IS - 3
ER -