CENH3 interacts with the centromeric retrotransposon cereba and GC-rich satellites and locates to centromeric substructures in barley

Andreas Houben, Elizabeth Schroeder-Reiter, Kiyotaka Nagaki, Shuhei Nasuda, Gerhard Wanner, Minoru Murata, Takashi R. Endo

    Research output: Contribution to journalArticlepeer-review

    80 Citations (Scopus)

    Abstract

    The chromosomal location of centromere-specific histone H3 (CENH3) is the assembly site for the kinetochore complex of active centromeres. Chromatin immunoprecipitation data indicated that CENH3 interacts in barley with cereba, a centromeric retroelement (CR)-like element conserved among cereal centromeres and barley-specific GC-rich centromeric satellite sequences. Anti-CENH3 signals on extended chromatin fibers always colocalized with the centromeric sequences but did not encompass the entire area covered by such centromeric repeats. This indicates that the CENH3 protein is bound only to a fraction of the centromeric repeats. At mitotic metaphase, CENH3, histone H3, and serine 10 phosphorylated histone H3 predominated within distinct structural subdomains of the centromere, as demonstrated by immunogold labeling for high resolution scanning electron microscopy.

    Original languageEnglish
    Pages (from-to)275-283
    Number of pages9
    JournalChromosoma
    Volume116
    Issue number3
    DOIs
    Publication statusPublished - Jun 2007

    ASJC Scopus subject areas

    • Genetics
    • Genetics(clinical)

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