Abstract
Background: In contrast to Staphylococcus aureus-derived superantigenic exotoxins, the role of nonsuperantigenic exotoxins in the pathogenesis of eosinophilic airway diseases remains obscure. We sought to characterize S. aureus alpha-toxin-induced cellular responses in chronic rhinosinusitis with nasal polyps (CRSwNP).
Methods: Dispersed nasal polyp cells and uncinate tissue cells were prepared from patients with CRS with and without nasal polyps, respectively. Cells were incubated with various concentrations of alpha-toxin or staphylococcal enterotoxin B and then the levels of IL-5, IL-13, IFN-γ, IL-17A, and IL-10 in the cell supernatants were determined. The pathophysiological significance of alpha-toxin-induced cytokine production was also determined including radiological severity of rhinosinusitis, tissue and blood eosinophilia, serum total IgE level, and 1-s forced expiratory volume[1]forced vital capacity ratio (FEV1/FVC).
Results: Nasal polyp cells produced substantial amounts of IL-5, IL-13, IFN-γ, IL-17A, and IL-10 in response to alpha-toxin. Cytokine production was higher in nasal polyp cells than in uncinate tissue cells. The potency of alpha-toxin in stimulating IL-5, IL-13, and IL-10 production was comparable to that of enterotoxin. Alpha-toxininduced IFN-γ, IL-17A, and IL-10 production significantly and negatively correlated with the degree of eosinophil infiltration into nasal polyps. Conversely, alpha-toxin-induced IFN-γ and IL-10 production significantly and positively correlated with FEV1/FVC. IL-10 production was significantly lower in asthmatic patients compared to non-asthmatics
Conclusions: S. aureus-derived alpha-toxin can provoke cellular responses in nasal polyps. These responses, especially failure to synthesize IL-10, may play a role in the pathophysiology of CRSwNP.
| Original language | English |
|---|---|
| Pages (from-to) | 563-573 |
| Number of pages | 11 |
| Journal | Allergology International |
| Volume | 63 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Alpha-toxin
- Chronic rhinosinusitis
- Eosinophil
- IL-10
- Nasal polyps
ASJC Scopus subject areas
- Immunology and Allergy
- Medicine(all)
Cite this
Cellular responses to Staphylococcus aureus alpha-toxin in chronic rhinosinusitis with nasal polyps. / Okano, Mitsuhiro; Fujiwara, Tazuko; Kariya, Shin; Higaki, Takaya; Haruna, Takenori; Matsushita, Osamu; Noda, Yohei; Makihara, Seiichiro; Kanai, Kengo; Noyama, Yasuyuki; Taniguchi, Masami; Nishizaki, Kazunori.
In: Allergology International, Vol. 63, No. 4, 2014, p. 563-573.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cellular responses to Staphylococcus aureus alpha-toxin in chronic rhinosinusitis with nasal polyps
AU - Okano, Mitsuhiro
AU - Fujiwara, Tazuko
AU - Kariya, Shin
AU - Higaki, Takaya
AU - Haruna, Takenori
AU - Matsushita, Osamu
AU - Noda, Yohei
AU - Makihara, Seiichiro
AU - Kanai, Kengo
AU - Noyama, Yasuyuki
AU - Taniguchi, Masami
AU - Nishizaki, Kazunori
PY - 2014
Y1 - 2014
N2 - Background: In contrast to Staphylococcus aureus-derived superantigenic exotoxins, the role of nonsuperantigenic exotoxins in the pathogenesis of eosinophilic airway diseases remains obscure. We sought to characterize S. aureus alpha-toxin-induced cellular responses in chronic rhinosinusitis with nasal polyps (CRSwNP).Methods: Dispersed nasal polyp cells and uncinate tissue cells were prepared from patients with CRS with and without nasal polyps, respectively. Cells were incubated with various concentrations of alpha-toxin or staphylococcal enterotoxin B and then the levels of IL-5, IL-13, IFN-γ, IL-17A, and IL-10 in the cell supernatants were determined. The pathophysiological significance of alpha-toxin-induced cytokine production was also determined including radiological severity of rhinosinusitis, tissue and blood eosinophilia, serum total IgE level, and 1-s forced expiratory volume[1]forced vital capacity ratio (FEV1/FVC).Results: Nasal polyp cells produced substantial amounts of IL-5, IL-13, IFN-γ, IL-17A, and IL-10 in response to alpha-toxin. Cytokine production was higher in nasal polyp cells than in uncinate tissue cells. The potency of alpha-toxin in stimulating IL-5, IL-13, and IL-10 production was comparable to that of enterotoxin. Alpha-toxininduced IFN-γ, IL-17A, and IL-10 production significantly and negatively correlated with the degree of eosinophil infiltration into nasal polyps. Conversely, alpha-toxin-induced IFN-γ and IL-10 production significantly and positively correlated with FEV1/FVC. IL-10 production was significantly lower in asthmatic patients compared to non-asthmaticsConclusions: S. aureus-derived alpha-toxin can provoke cellular responses in nasal polyps. These responses, especially failure to synthesize IL-10, may play a role in the pathophysiology of CRSwNP.
AB - Background: In contrast to Staphylococcus aureus-derived superantigenic exotoxins, the role of nonsuperantigenic exotoxins in the pathogenesis of eosinophilic airway diseases remains obscure. We sought to characterize S. aureus alpha-toxin-induced cellular responses in chronic rhinosinusitis with nasal polyps (CRSwNP).Methods: Dispersed nasal polyp cells and uncinate tissue cells were prepared from patients with CRS with and without nasal polyps, respectively. Cells were incubated with various concentrations of alpha-toxin or staphylococcal enterotoxin B and then the levels of IL-5, IL-13, IFN-γ, IL-17A, and IL-10 in the cell supernatants were determined. The pathophysiological significance of alpha-toxin-induced cytokine production was also determined including radiological severity of rhinosinusitis, tissue and blood eosinophilia, serum total IgE level, and 1-s forced expiratory volume[1]forced vital capacity ratio (FEV1/FVC).Results: Nasal polyp cells produced substantial amounts of IL-5, IL-13, IFN-γ, IL-17A, and IL-10 in response to alpha-toxin. Cytokine production was higher in nasal polyp cells than in uncinate tissue cells. The potency of alpha-toxin in stimulating IL-5, IL-13, and IL-10 production was comparable to that of enterotoxin. Alpha-toxininduced IFN-γ, IL-17A, and IL-10 production significantly and negatively correlated with the degree of eosinophil infiltration into nasal polyps. Conversely, alpha-toxin-induced IFN-γ and IL-10 production significantly and positively correlated with FEV1/FVC. IL-10 production was significantly lower in asthmatic patients compared to non-asthmaticsConclusions: S. aureus-derived alpha-toxin can provoke cellular responses in nasal polyps. These responses, especially failure to synthesize IL-10, may play a role in the pathophysiology of CRSwNP.
KW - Alpha-toxin
KW - Chronic rhinosinusitis
KW - Eosinophil
KW - IL-10
KW - Nasal polyps
UR - http://www.scopus.com/inward/record.url?scp=84924806512&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84924806512&partnerID=8YFLogxK
U2 - 10.2332/allergolint.14-OA-0703
DO - 10.2332/allergolint.14-OA-0703
M3 - Article
C2 - 25056228
AN - SCOPUS:84924806512
VL - 63
SP - 563
EP - 573
JO - Allergology International
JF - Allergology International
SN - 1323-8930
IS - 4
ER -