Cellular radiosensitivity and cell-type-specific activation of apoptosis signaling pathways

Fumio Suzuki, Yukimi Akimoto, Kaori Sasai, Hirohiko Yajima

Research output: Contribution to journalArticle

Abstract

We have examined the cell-type-dependent sensitivity to UV-induced apoptosis using mouse lymphoma 3SB and human leukemic Jurkat cells and three human carcinoma cell lines (HeLa S3, HeLa SC and MCF-7). Exposure of 3SB and Jurkat cells resulted in large amounts of cytochrome c being released into the cytosol, and a clear laddering pattern of DNA fragments and morphological changes characteristic of apoptosis were detected within 3 h of incubation after irradiation. HeLa S3, HeLa SC and MCF-7 cells also showed extensive release of cytochrome c at 4-6 h after exposure, but apoptotic morphologic changes appeared much later. Similar differences in induction of apoptosis after γ irradiation were observed between two kinds of cells. Interestingly, 3SB cells that express wild-type p53 protein showed a steep increase in number of apoptotic cells when irradiated with low doses of γ-rays, and the dose response of apoptosis induction was not influenced by radiation dose rate. This result suggests that signaling for radiation-induced apoptosis in hematopoietic cells showing rapid apoptosis can be triggered by initial DNA damage, and not determined by DNA repair.

Original languageEnglish
Pages (from-to)233-237
Number of pages5
JournalInternational Congress Series
Volume1258
Issue numberC
DOIs
Publication statusPublished - Nov 1 2003
Externally publishedYes

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Keywords

  • Apoptosis
  • Dose rate
  • Hematopoietic cell
  • UV
  • γ-rays

ASJC Scopus subject areas

  • Medicine(all)

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