Cellular radiosensitivity and cell-type-specific activation of apoptosis signaling pathways

Fumio Suzuki, Yukimi Akimoto, Kaori Sasai, Hirohiko Yajima

Research output: Contribution to journalArticle

Abstract

We have examined the cell-type-dependent sensitivity to UV-induced apoptosis using mouse lymphoma 3SB and human leukemic Jurkat cells and three human carcinoma cell lines (HeLa S3, HeLa SC and MCF-7). Exposure of 3SB and Jurkat cells resulted in large amounts of cytochrome c being released into the cytosol, and a clear laddering pattern of DNA fragments and morphological changes characteristic of apoptosis were detected within 3 h of incubation after irradiation. HeLa S3, HeLa SC and MCF-7 cells also showed extensive release of cytochrome c at 4-6 h after exposure, but apoptotic morphologic changes appeared much later. Similar differences in induction of apoptosis after γ irradiation were observed between two kinds of cells. Interestingly, 3SB cells that express wild-type p53 protein showed a steep increase in number of apoptotic cells when irradiated with low doses of γ-rays, and the dose response of apoptosis induction was not influenced by radiation dose rate. This result suggests that signaling for radiation-induced apoptosis in hematopoietic cells showing rapid apoptosis can be triggered by initial DNA damage, and not determined by DNA repair.

Original languageEnglish
Pages (from-to)233-237
Number of pages5
JournalInternational Congress Series
Volume1258
Issue numberC
DOIs
Publication statusPublished - Nov 1 2003
Externally publishedYes

Fingerprint

Radiation Tolerance
Apoptosis
Jurkat Cells
Cytochromes c
Radiation
MCF-7 Cells
DNA Repair
Cytosol
DNA Damage
Lymphoma
Cell Count
Carcinoma
Cell Line
DNA
Proteins

Keywords

  • Apoptosis
  • Dose rate
  • Hematopoietic cell
  • UV
  • γ-rays

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cellular radiosensitivity and cell-type-specific activation of apoptosis signaling pathways. / Suzuki, Fumio; Akimoto, Yukimi; Sasai, Kaori; Yajima, Hirohiko.

In: International Congress Series, Vol. 1258, No. C, 01.11.2003, p. 233-237.

Research output: Contribution to journalArticle

Suzuki, Fumio ; Akimoto, Yukimi ; Sasai, Kaori ; Yajima, Hirohiko. / Cellular radiosensitivity and cell-type-specific activation of apoptosis signaling pathways. In: International Congress Series. 2003 ; Vol. 1258, No. C. pp. 233-237.
@article{e5546deeaa0047a2bdd072ed9bcc1310,
title = "Cellular radiosensitivity and cell-type-specific activation of apoptosis signaling pathways",
abstract = "We have examined the cell-type-dependent sensitivity to UV-induced apoptosis using mouse lymphoma 3SB and human leukemic Jurkat cells and three human carcinoma cell lines (HeLa S3, HeLa SC and MCF-7). Exposure of 3SB and Jurkat cells resulted in large amounts of cytochrome c being released into the cytosol, and a clear laddering pattern of DNA fragments and morphological changes characteristic of apoptosis were detected within 3 h of incubation after irradiation. HeLa S3, HeLa SC and MCF-7 cells also showed extensive release of cytochrome c at 4-6 h after exposure, but apoptotic morphologic changes appeared much later. Similar differences in induction of apoptosis after γ irradiation were observed between two kinds of cells. Interestingly, 3SB cells that express wild-type p53 protein showed a steep increase in number of apoptotic cells when irradiated with low doses of γ-rays, and the dose response of apoptosis induction was not influenced by radiation dose rate. This result suggests that signaling for radiation-induced apoptosis in hematopoietic cells showing rapid apoptosis can be triggered by initial DNA damage, and not determined by DNA repair.",
keywords = "Apoptosis, Dose rate, Hematopoietic cell, UV, γ-rays",
author = "Fumio Suzuki and Yukimi Akimoto and Kaori Sasai and Hirohiko Yajima",
year = "2003",
month = "11",
day = "1",
doi = "10.1016/S0531-5131(03)01214-7",
language = "English",
volume = "1258",
pages = "233--237",
journal = "International Congress Series",
issn = "0531-5131",
publisher = "Elsevier BV",
number = "C",

}

TY - JOUR

T1 - Cellular radiosensitivity and cell-type-specific activation of apoptosis signaling pathways

AU - Suzuki, Fumio

AU - Akimoto, Yukimi

AU - Sasai, Kaori

AU - Yajima, Hirohiko

PY - 2003/11/1

Y1 - 2003/11/1

N2 - We have examined the cell-type-dependent sensitivity to UV-induced apoptosis using mouse lymphoma 3SB and human leukemic Jurkat cells and three human carcinoma cell lines (HeLa S3, HeLa SC and MCF-7). Exposure of 3SB and Jurkat cells resulted in large amounts of cytochrome c being released into the cytosol, and a clear laddering pattern of DNA fragments and morphological changes characteristic of apoptosis were detected within 3 h of incubation after irradiation. HeLa S3, HeLa SC and MCF-7 cells also showed extensive release of cytochrome c at 4-6 h after exposure, but apoptotic morphologic changes appeared much later. Similar differences in induction of apoptosis after γ irradiation were observed between two kinds of cells. Interestingly, 3SB cells that express wild-type p53 protein showed a steep increase in number of apoptotic cells when irradiated with low doses of γ-rays, and the dose response of apoptosis induction was not influenced by radiation dose rate. This result suggests that signaling for radiation-induced apoptosis in hematopoietic cells showing rapid apoptosis can be triggered by initial DNA damage, and not determined by DNA repair.

AB - We have examined the cell-type-dependent sensitivity to UV-induced apoptosis using mouse lymphoma 3SB and human leukemic Jurkat cells and three human carcinoma cell lines (HeLa S3, HeLa SC and MCF-7). Exposure of 3SB and Jurkat cells resulted in large amounts of cytochrome c being released into the cytosol, and a clear laddering pattern of DNA fragments and morphological changes characteristic of apoptosis were detected within 3 h of incubation after irradiation. HeLa S3, HeLa SC and MCF-7 cells also showed extensive release of cytochrome c at 4-6 h after exposure, but apoptotic morphologic changes appeared much later. Similar differences in induction of apoptosis after γ irradiation were observed between two kinds of cells. Interestingly, 3SB cells that express wild-type p53 protein showed a steep increase in number of apoptotic cells when irradiated with low doses of γ-rays, and the dose response of apoptosis induction was not influenced by radiation dose rate. This result suggests that signaling for radiation-induced apoptosis in hematopoietic cells showing rapid apoptosis can be triggered by initial DNA damage, and not determined by DNA repair.

KW - Apoptosis

KW - Dose rate

KW - Hematopoietic cell

KW - UV

KW - γ-rays

UR - http://www.scopus.com/inward/record.url?scp=85023005695&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85023005695&partnerID=8YFLogxK

U2 - 10.1016/S0531-5131(03)01214-7

DO - 10.1016/S0531-5131(03)01214-7

M3 - Article

AN - SCOPUS:85023005695

VL - 1258

SP - 233

EP - 237

JO - International Congress Series

JF - International Congress Series

SN - 0531-5131

IS - C

ER -