Cellular growth defects triggered by an overload of protein localization processes

Reiko Kintaka, Koji Makanae, Hisao Moriya

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

High-level expression of a protein localized to an intracellular compartment is expected to cause cellular defects because it overloads localization processes. However, overloads of localization processes have never been studied systematically. Here, we show that the expression levels of green fluorescent proteins (GFPs) with localization signals were limited to the same degree as a toxic misfolded GFP in budding yeast cells, and that their high-level expression caused cellular defects associated with localization processes. We further show that limitation of the exportin Crm1 determined the expression limit of GFP with a nuclear export signal. Although misfolding of GFP with a vesicle-mediated transport signal triggered endoplasmic reticulum stress, it was not the primary determinant of its expression limit. The precursor of GFP with a mitochondrial targeting signal caused a cellular defect. Finally, we estimated the residual capacities of localization processes. High-level expression of a localized protein thus causes cellular defects by overloading the capacities of localization processes.

Original languageEnglish
Article number31774
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - Aug 19 2016

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Green Fluorescent Proteins
Growth
Proteins
Nuclear Export Signals
Karyopherins
Transport Vesicles
Saccharomycetales
Endoplasmic Reticulum Stress
Poisons

ASJC Scopus subject areas

  • General

Cite this

Cellular growth defects triggered by an overload of protein localization processes. / Kintaka, Reiko; Makanae, Koji; Moriya, Hisao.

In: Scientific Reports, Vol. 6, 31774, 19.08.2016.

Research output: Contribution to journalArticle

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