Cellular Fluorescence Imaging for the Evaluation of Bioactivity of CCN Family Proteins

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The method of labeling proteins of interest with fluorescent dyes that can specifically stain organelles in living cells provides a tool for investigating various cellular processes under a microscope. Visualization (imaging) of the cells using fluorescence has many advantages, including the ability to stain multiple cell organelles and intracellular proteins simultaneously and discriminately, and is used in many research fields. In this chapter, we describe the observation of cell organelles using fluorescence staining to analyze the functions of CCN family proteins involved in various cellular events.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages23-29
Number of pages7
DOIs
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2582
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Fluorescence microscopy
  • Fluorescent labeling
  • Live-cell imaging
  • Metabolic engineering
  • Organelle

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Cellular Fluorescence Imaging for the Evaluation of Bioactivity of CCN Family Proteins'. Together they form a unique fingerprint.

Cite this