Cellular energization protects the photosynthetic machinery against salt-induced inactivation in Synechococcus

Suleyman Allakhverdiev, Vyacheslav V. Klimov, Martin Hagemann

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The effects of the energization of cells by light and by exogenous glucose on the salt-induced inactivation of the photosynthetic machinery were investigated in the cyanobacterium Synechococcus sp. PCC 7942. The incubation of the cyanobacterial cells in a medium supplemented with 0.5 M NaCl induced a rapid decline with a subsequent slow decline, in the oxygen-evolving activity of Photosystem (PS) II and in the electron-transport activity of PSI. Light and exogenous glucose each protected PSII and PSI against the second phase of the NaCl-induced inactivation. The protective effects of light and glucose were eliminated by an uncoupler of phosphorylation and by lincomycin, an inhibitor of protein synthesis. Light and glucose had similar effects on the NaCl-induced inactivation of Na+/H+ antiporters. After photosynthetic and Na+/H+-antiport activities had been eliminated by the exposure of cells to 0.5 M NaCl in the darkness, both activities were partially restored by light or exogenous glucose. This recovery was prevented by lincomycin. These observations suggest that cellular energization by either photosynthesis or respiration, which is necessary for protein synthesis, is important for the recovery of the photosynthetic machinery and Na +/H+ antiporters from inactivation by a high level of NaCl.

Original languageEnglish
Pages (from-to)201-208
Number of pages8
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1708
Issue number2
DOIs
Publication statusPublished - Jun 30 2005
Externally publishedYes

Fingerprint

Synechococcus
Machinery
Salts
Light
Glucose
Lincomycin
Sodium-Hydrogen Antiporter
Recovery
Phosphorylation
Protein Synthesis Inhibitors
Photosystem II Protein Complex
Photosynthesis
Darkness
Ion Transport
Cyanobacteria
Electron Transport
Respiration
Cells
Oxygen
Proteins

Keywords

  • Cellular energization
  • Na /H-antiporter
  • Photosynthesis
  • Respiration
  • Salt stress
  • Synechococcus

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Cellular energization protects the photosynthetic machinery against salt-induced inactivation in Synechococcus. / Allakhverdiev, Suleyman; Klimov, Vyacheslav V.; Hagemann, Martin.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1708, No. 2, 30.06.2005, p. 201-208.

Research output: Contribution to journalArticle

Allakhverdiev, Suleyman ; Klimov, Vyacheslav V. ; Hagemann, Martin. / Cellular energization protects the photosynthetic machinery against salt-induced inactivation in Synechococcus. In: Biochimica et Biophysica Acta - Bioenergetics. 2005 ; Vol. 1708, No. 2. pp. 201-208.
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