Cell wall related gene expression during secondary physiological fruit drop in ponkan (Citrus reticulata Blanco) and hyuganatsu (C. tamurana hort. ex Tanaka)

X. Li, A. Kitajima, K. Katsura, H. Saito, S. Koeda, R. Takisawa, Takashi Kawai, T. Nakazaki, T. Shimizu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Physiological fruit drop in citrus is the deeper problem for fruit production and many researches have suggested that decreasing of carbohydrate translocation to fruit involved in fruit abscission. The fruit abscission process requires 3 stages for successful abscission, which include "induction", "decision", and "execution". However, there is almost no information in the molecular mechanism of secondary physiological fruit drop in citrus. Here, anatomical observation and microarray analysis demonstrated the candidate genes involved in the fruit abscission process after induction of fruit abscission by incubating the detached ponkan (Citrus reticulata Blanco) and hyuganatsu (C. tamurana hort. ex Tanaka) fruit on agar medium. In our study, the cumulative ratio of fruit abscission was very high in ponkan, but very low in hyuganatsu. Morphology of abscission zone (AZ) cells was drastically changed in ponkan, but almost not changed in hyuganatsu. From the 60 K microarray analysis at 0, 12, 24, or 36 h after the induction, the specific up-regulated genes in ponkan AZ tissue were identified. The expressions of many cell wall degrading related genes, such as beta-galactosidase, polygalacturonase and beta-glucosidase etc. increased rapidly at 24 h. These results indicated that "execution" stage of fruit abscission process would be initiated until 24 h after the induction of fruit abscission in citrus.

Original languageEnglish
Title of host publication3rd International Symposium on Citrus Biotechnology
PublisherInternational Society for Horticultural Science
Pages47-52
Number of pages6
Volume1135
ISBN (Electronic)9789462611207
DOIs
Publication statusPublished - Jul 1 2016
Externally publishedYes

Publication series

NameActa Horticulturae
Volume1135
ISSN (Print)0567-7572

Fingerprint

Citrus reticulata
fruit drop
cell walls
gene expression
abscission
Citrus
fruits
genes
polygalacturonase
beta-galactosidase
beta-glucosidase
fruiting
agar
carbohydrates

Keywords

  • Cell wall
  • Fruit abscission
  • Genes
  • Microarray

ASJC Scopus subject areas

  • Horticulture

Cite this

Li, X., Kitajima, A., Katsura, K., Saito, H., Koeda, S., Takisawa, R., ... Shimizu, T. (2016). Cell wall related gene expression during secondary physiological fruit drop in ponkan (Citrus reticulata Blanco) and hyuganatsu (C. tamurana hort. ex Tanaka). In 3rd International Symposium on Citrus Biotechnology (Vol. 1135, pp. 47-52). (Acta Horticulturae; Vol. 1135). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.2016.1135.6

Cell wall related gene expression during secondary physiological fruit drop in ponkan (Citrus reticulata Blanco) and hyuganatsu (C. tamurana hort. ex Tanaka). / Li, X.; Kitajima, A.; Katsura, K.; Saito, H.; Koeda, S.; Takisawa, R.; Kawai, Takashi; Nakazaki, T.; Shimizu, T.

3rd International Symposium on Citrus Biotechnology. Vol. 1135 International Society for Horticultural Science, 2016. p. 47-52 (Acta Horticulturae; Vol. 1135).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Li, X, Kitajima, A, Katsura, K, Saito, H, Koeda, S, Takisawa, R, Kawai, T, Nakazaki, T & Shimizu, T 2016, Cell wall related gene expression during secondary physiological fruit drop in ponkan (Citrus reticulata Blanco) and hyuganatsu (C. tamurana hort. ex Tanaka). in 3rd International Symposium on Citrus Biotechnology. vol. 1135, Acta Horticulturae, vol. 1135, International Society for Horticultural Science, pp. 47-52. https://doi.org/10.17660/ActaHortic.2016.1135.6
Li X, Kitajima A, Katsura K, Saito H, Koeda S, Takisawa R et al. Cell wall related gene expression during secondary physiological fruit drop in ponkan (Citrus reticulata Blanco) and hyuganatsu (C. tamurana hort. ex Tanaka). In 3rd International Symposium on Citrus Biotechnology. Vol. 1135. International Society for Horticultural Science. 2016. p. 47-52. (Acta Horticulturae). https://doi.org/10.17660/ActaHortic.2016.1135.6
Li, X. ; Kitajima, A. ; Katsura, K. ; Saito, H. ; Koeda, S. ; Takisawa, R. ; Kawai, Takashi ; Nakazaki, T. ; Shimizu, T. / Cell wall related gene expression during secondary physiological fruit drop in ponkan (Citrus reticulata Blanco) and hyuganatsu (C. tamurana hort. ex Tanaka). 3rd International Symposium on Citrus Biotechnology. Vol. 1135 International Society for Horticultural Science, 2016. pp. 47-52 (Acta Horticulturae).
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