Cell-type specificity of interleukins 1α and 1β on prostaglandin and plasminogen activator production in bovine endometrial cells

Michiyo Tanikawa, Tae Shin Kim, Kiyoshi Okuda, Zae Young Ryoo, Soo Bong Park, Jae Ho Shin, Choon Keun Park, Dong Seok Lee

Research output: Contribution to journalArticle

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Abstract

Interleukin (IL)-1α is a potent stimulator of prostaglandin production in bovine endometrium, and IL-1 affects plasminogen activator (PA) activity in several types of cells. In this study, we determined the effects of IL-1α and IL-1β on production of the prostaglandins PGF and PGE2 and on PA activity in cultured bovine endometrial epithelial and stromal cells. We also determined the effects of PGE2 and PGF on PA activity in these cells. Finally, we used RT-PCR to examine the expression of IL-1α, IL-1β, and IL-1 receptor type 1 (IL-1R) mRNA in cultured bovine endometrial cells. This analysis revealed that IL-1α mRNA was present only in the stromal cells, whereas IL-1β and IL-1R mRNAs were present in both cell types. When cultured cells were exposed to IL-1α and IL-1β at concentrations ranging from 0.006 to 3 nM for 24 h, IL-1α and IL-1β were found to dose-dependently stimulate PGE2 and PGF production in stromal cells (P <0.05) but not in epithelial cells. On the other hand, exposure to IL-1α and IL-1β dose-dependently increased PA activity in the epithelial cells, whereas neither stimulated PA production in the stromal cells. When cells were exposed to IL-1α and IL-1β at concentrations ranging from 0.06 to 3 nM for 24 h, the two IL-1s differed in their effects on both PGE2 and PGF production in stromal cells and had significantly differed in their effects on PA activity in epithelial cells. Exposure to PGE2 and PGF did not affect PA activity in either stromal or epithelial cells (P > 0.05). Taken together, these results suggest the possibility that both IL-1α and IL-1β are produced by the stromal cells, that IL-1β is produced by the epithelial cells, and that IL-1α is a far more potent stimulator than IL-1β of prostaglandin and PA production in cultured bovine endometrial epithelial and stromal cells.

Original languageEnglish
Pages (from-to)32-42
Number of pages11
JournalAnimal Reproduction Science
Volume114
Issue number1-3
DOIs
Publication statusPublished - Aug 2009

Fingerprint

plasminogen activator
Plasminogen Activators
interleukin-1
Interleukin-1
prostaglandins
Prostaglandins
cattle
cells
stromal cells
Stromal Cells
Dinoprost
Dinoprostone
epithelial cells
Epithelial Cells
Messenger RNA
Interleukin-1 Type I Receptors
Interleukins
endometrium

Keywords

  • Cattle
  • Endometrial cells
  • Interleukin-1
  • Plasminogen activator
  • Prostaglandins

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Animals
  • Endocrinology

Cite this

Cell-type specificity of interleukins 1α and 1β on prostaglandin and plasminogen activator production in bovine endometrial cells. / Tanikawa, Michiyo; Kim, Tae Shin; Okuda, Kiyoshi; Ryoo, Zae Young; Park, Soo Bong; Shin, Jae Ho; Park, Choon Keun; Lee, Dong Seok.

In: Animal Reproduction Science, Vol. 114, No. 1-3, 08.2009, p. 32-42.

Research output: Contribution to journalArticle

Tanikawa, Michiyo ; Kim, Tae Shin ; Okuda, Kiyoshi ; Ryoo, Zae Young ; Park, Soo Bong ; Shin, Jae Ho ; Park, Choon Keun ; Lee, Dong Seok. / Cell-type specificity of interleukins 1α and 1β on prostaglandin and plasminogen activator production in bovine endometrial cells. In: Animal Reproduction Science. 2009 ; Vol. 114, No. 1-3. pp. 32-42.
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abstract = "Interleukin (IL)-1α is a potent stimulator of prostaglandin production in bovine endometrium, and IL-1 affects plasminogen activator (PA) activity in several types of cells. In this study, we determined the effects of IL-1α and IL-1β on production of the prostaglandins PGF2α and PGE2 and on PA activity in cultured bovine endometrial epithelial and stromal cells. We also determined the effects of PGE2 and PGF2α on PA activity in these cells. Finally, we used RT-PCR to examine the expression of IL-1α, IL-1β, and IL-1 receptor type 1 (IL-1R) mRNA in cultured bovine endometrial cells. This analysis revealed that IL-1α mRNA was present only in the stromal cells, whereas IL-1β and IL-1R mRNAs were present in both cell types. When cultured cells were exposed to IL-1α and IL-1β at concentrations ranging from 0.006 to 3 nM for 24 h, IL-1α and IL-1β were found to dose-dependently stimulate PGE2 and PGF2α production in stromal cells (P <0.05) but not in epithelial cells. On the other hand, exposure to IL-1α and IL-1β dose-dependently increased PA activity in the epithelial cells, whereas neither stimulated PA production in the stromal cells. When cells were exposed to IL-1α and IL-1β at concentrations ranging from 0.06 to 3 nM for 24 h, the two IL-1s differed in their effects on both PGE2 and PGF2α production in stromal cells and had significantly differed in their effects on PA activity in epithelial cells. Exposure to PGE2 and PGF2α did not affect PA activity in either stromal or epithelial cells (P > 0.05). Taken together, these results suggest the possibility that both IL-1α and IL-1β are produced by the stromal cells, that IL-1β is produced by the epithelial cells, and that IL-1α is a far more potent stimulator than IL-1β of prostaglandin and PA production in cultured bovine endometrial epithelial and stromal cells.",
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AU - Tanikawa, Michiyo

AU - Kim, Tae Shin

AU - Okuda, Kiyoshi

AU - Ryoo, Zae Young

AU - Park, Soo Bong

AU - Shin, Jae Ho

AU - Park, Choon Keun

AU - Lee, Dong Seok

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N2 - Interleukin (IL)-1α is a potent stimulator of prostaglandin production in bovine endometrium, and IL-1 affects plasminogen activator (PA) activity in several types of cells. In this study, we determined the effects of IL-1α and IL-1β on production of the prostaglandins PGF2α and PGE2 and on PA activity in cultured bovine endometrial epithelial and stromal cells. We also determined the effects of PGE2 and PGF2α on PA activity in these cells. Finally, we used RT-PCR to examine the expression of IL-1α, IL-1β, and IL-1 receptor type 1 (IL-1R) mRNA in cultured bovine endometrial cells. This analysis revealed that IL-1α mRNA was present only in the stromal cells, whereas IL-1β and IL-1R mRNAs were present in both cell types. When cultured cells were exposed to IL-1α and IL-1β at concentrations ranging from 0.006 to 3 nM for 24 h, IL-1α and IL-1β were found to dose-dependently stimulate PGE2 and PGF2α production in stromal cells (P <0.05) but not in epithelial cells. On the other hand, exposure to IL-1α and IL-1β dose-dependently increased PA activity in the epithelial cells, whereas neither stimulated PA production in the stromal cells. When cells were exposed to IL-1α and IL-1β at concentrations ranging from 0.06 to 3 nM for 24 h, the two IL-1s differed in their effects on both PGE2 and PGF2α production in stromal cells and had significantly differed in their effects on PA activity in epithelial cells. Exposure to PGE2 and PGF2α did not affect PA activity in either stromal or epithelial cells (P > 0.05). Taken together, these results suggest the possibility that both IL-1α and IL-1β are produced by the stromal cells, that IL-1β is produced by the epithelial cells, and that IL-1α is a far more potent stimulator than IL-1β of prostaglandin and PA production in cultured bovine endometrial epithelial and stromal cells.

AB - Interleukin (IL)-1α is a potent stimulator of prostaglandin production in bovine endometrium, and IL-1 affects plasminogen activator (PA) activity in several types of cells. In this study, we determined the effects of IL-1α and IL-1β on production of the prostaglandins PGF2α and PGE2 and on PA activity in cultured bovine endometrial epithelial and stromal cells. We also determined the effects of PGE2 and PGF2α on PA activity in these cells. Finally, we used RT-PCR to examine the expression of IL-1α, IL-1β, and IL-1 receptor type 1 (IL-1R) mRNA in cultured bovine endometrial cells. This analysis revealed that IL-1α mRNA was present only in the stromal cells, whereas IL-1β and IL-1R mRNAs were present in both cell types. When cultured cells were exposed to IL-1α and IL-1β at concentrations ranging from 0.006 to 3 nM for 24 h, IL-1α and IL-1β were found to dose-dependently stimulate PGE2 and PGF2α production in stromal cells (P <0.05) but not in epithelial cells. On the other hand, exposure to IL-1α and IL-1β dose-dependently increased PA activity in the epithelial cells, whereas neither stimulated PA production in the stromal cells. When cells were exposed to IL-1α and IL-1β at concentrations ranging from 0.06 to 3 nM for 24 h, the two IL-1s differed in their effects on both PGE2 and PGF2α production in stromal cells and had significantly differed in their effects on PA activity in epithelial cells. Exposure to PGE2 and PGF2α did not affect PA activity in either stromal or epithelial cells (P > 0.05). Taken together, these results suggest the possibility that both IL-1α and IL-1β are produced by the stromal cells, that IL-1β is produced by the epithelial cells, and that IL-1α is a far more potent stimulator than IL-1β of prostaglandin and PA production in cultured bovine endometrial epithelial and stromal cells.

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