Cell surface protein antigen from Wolinella recta ATCC 33238(T)

Susumu Kokeguchi, K. Kato, H. Kurihara, Y. Murayama

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A high-molecular-weight (approximately 150,000) protein selectively isolated by acid extraction from the cell surface of Wolinella recta and purified by negative adsorption on DEAE-cellulose and gel filtration. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that this protein was found in W. recta but not in other Wolinella species, such as W. curva and W. succinogenes. Sera from patients with periodontitis reacted strongly with this protein antigen, whereas sera from healthy donors showed little or no reactivity, as determined by immunoblotting analysis. In serum, titers of immunoglobulin G antibodies to the protein antigen were significantly higher in patients with periodonitis than in periodontally healthy donors, as detected by an enzyme-linked immunosorbent assay.

Original languageEnglish
Pages (from-to)1210-1217
Number of pages8
JournalJournal of Clinical Microbiology
Volume27
Issue number6
Publication statusPublished - 1989

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Wolinella
Surface Antigens
Rectum
Membrane Proteins
Proteins
Serum
Tissue Donors
Antigens
DEAE-Cellulose
Periodontitis
Immunoblotting
Sodium Dodecyl Sulfate
Adsorption
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Immunoglobulin G
Molecular Weight
Enzyme-Linked Immunosorbent Assay
Acids
Antibodies

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Cell surface protein antigen from Wolinella recta ATCC 33238(T). / Kokeguchi, Susumu; Kato, K.; Kurihara, H.; Murayama, Y.

In: Journal of Clinical Microbiology, Vol. 27, No. 6, 1989, p. 1210-1217.

Research output: Contribution to journalArticle

Kokeguchi, S, Kato, K, Kurihara, H & Murayama, Y 1989, 'Cell surface protein antigen from Wolinella recta ATCC 33238(T)', Journal of Clinical Microbiology, vol. 27, no. 6, pp. 1210-1217.
Kokeguchi, Susumu ; Kato, K. ; Kurihara, H. ; Murayama, Y. / Cell surface protein antigen from Wolinella recta ATCC 33238(T). In: Journal of Clinical Microbiology. 1989 ; Vol. 27, No. 6. pp. 1210-1217.
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