Cell surface-associated enolase in Actinobacillus actinomycetemcomitans

Hiroaki Hara, Hiroyuki Ohta, Tetsuyoshi Inoue, Toshio Ohashi, Shogo Takashiba, Yoji Murayama, Kazuhiro Fukui

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Cell surface-associated materials of Actinobacillus actinomycetemcomitans were extracted by a short incubation of the cell suspension in a Tris-buffered saline in the presence and absence of a restriction enzyme, EcoRI. The supernatants (which we termed EcoRI extract and surface extract, respectively) contained a number of extracellularly released proteins. Of these proteins, four major proteins were identified by N-terminal sequencing to be the 34 and 39 kDa outer membrane proteins, the GroEL-like protein, and a 47 kDa protein homologous to Haemophilus influenzae enolase. Enolase activity was found in the extracts and its relative amount of activity in the EcoRI extract from a culture of the mid-exponential growth phase was estimated as 5.7% of total enzyme activity. In contrast, tile relative amount of activity of another cytosolic enzyme, lactate dehydrogenase, was extremely low in the extracts and also in the culture supernatant. These results suggest the external localization of enolase in this bacterium.

Original languageEnglish
Pages (from-to)349-356
Number of pages8
JournalMICROBIOLOGY and IMMUNOLOGY
Volume44
Issue number5
DOIs
Publication statusPublished - 2000

Keywords

  • Actinobacillus actinomycetemcomitans
  • Cell surface
  • Enolase

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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