Cell surface-associated enolase in Actinobacillus actinomycetemcomitans

Hiroaki Hara, Hiroyuki Ohta, Tetsuyoshi Inoue, Toshio Ohashi, Shogo Takashiba, Yoji Murayama, Kazuhiro Fukui

Research output: Contribution to journalArticle

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Abstract

Cell surface-associated materials of Actinobacillus actinomycetemcomitans were extracted by a short incubation of the cell suspension in a Tris-buffered saline in the presence and absence of a restriction enzyme, EcoRI. The supernatants (which we termed EcoRI extract and surface extract, respectively) contained a number of extracellularly released proteins. Of these proteins, four major proteins were identified by N-terminal sequencing to be the 34 and 39 kDa outer membrane proteins, the GroEL-like protein, and a 47 kDa protein homologous to Haemophilus influenzae enolase. Enolase activity was found in the extracts and its relative amount of activity in the EcoRI extract from a culture of the mid-exponential growth phase was estimated as 5.7% of total enzyme activity. In contrast, tile relative amount of activity of another cytosolic enzyme, lactate dehydrogenase, was extremely low in the extracts and also in the culture supernatant. These results suggest the external localization of enolase in this bacterium.

Original languageEnglish
Pages (from-to)349-356
Number of pages8
JournalMicrobiology and Immunology
Volume44
Issue number5
Publication statusPublished - 2000

Fingerprint

Aggregatibacter actinomycetemcomitans
Phosphopyruvate Hydratase
Proteins
Enzymes
Chaperonin 60
Haemophilus influenzae
L-Lactate Dehydrogenase
Suspensions
Membrane Proteins
Bacteria
Growth

Keywords

  • Actinobacillus actinomycetemcomitans
  • Cell surface
  • Enolase

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Hara, H., Ohta, H., Inoue, T., Ohashi, T., Takashiba, S., Murayama, Y., & Fukui, K. (2000). Cell surface-associated enolase in Actinobacillus actinomycetemcomitans. Microbiology and Immunology, 44(5), 349-356.

Cell surface-associated enolase in Actinobacillus actinomycetemcomitans. / Hara, Hiroaki; Ohta, Hiroyuki; Inoue, Tetsuyoshi; Ohashi, Toshio; Takashiba, Shogo; Murayama, Yoji; Fukui, Kazuhiro.

In: Microbiology and Immunology, Vol. 44, No. 5, 2000, p. 349-356.

Research output: Contribution to journalArticle

Hara, H, Ohta, H, Inoue, T, Ohashi, T, Takashiba, S, Murayama, Y & Fukui, K 2000, 'Cell surface-associated enolase in Actinobacillus actinomycetemcomitans', Microbiology and Immunology, vol. 44, no. 5, pp. 349-356.
Hara H, Ohta H, Inoue T, Ohashi T, Takashiba S, Murayama Y et al. Cell surface-associated enolase in Actinobacillus actinomycetemcomitans. Microbiology and Immunology. 2000;44(5):349-356.
Hara, Hiroaki ; Ohta, Hiroyuki ; Inoue, Tetsuyoshi ; Ohashi, Toshio ; Takashiba, Shogo ; Murayama, Yoji ; Fukui, Kazuhiro. / Cell surface-associated enolase in Actinobacillus actinomycetemcomitans. In: Microbiology and Immunology. 2000 ; Vol. 44, No. 5. pp. 349-356.
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