Cell Surface-Anchored Fluorescent Probe Capable of Real-Time Imaging of Single Mast Cell Degranulation Based on Histamine-Induced Coordination Displacement

Yuji Oshikawa, Kazuyuki Furuta, Satoshi Tanaka, Akio Ojida

Research output: Contribution to journalArticle

9 Citations (Scopus)


Mast cells secrete histamine upon degranulation triggered by various stimuli. Herein, we report the new detection method of mast cell degranulation using the fluorescent probe capable of detection of the released histamine. The probe was designed as the Co(II) complex of a cyanine dye, which shows a turn-on fluorescence signal based on a histamine-induced coordination displacement mechanism. Fluorescence imaging using the cell surface-anchored fluorescent probe enabled the real-time detection of mast cell degranulation induced by various secretagogues.

Original languageEnglish
Pages (from-to)1526-1529
Number of pages4
JournalAnalytical Chemistry
Issue number3
Publication statusPublished - Feb 2 2016


ASJC Scopus subject areas

  • Analytical Chemistry

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