Cell-penetrating peptide as a means of directing the differentiation of induced-pluripotent stem cells

Taku Kaitsuka, Kazuhito Tomizawa

    Research output: Contribution to journalReview articlepeer-review

    14 Citations (Scopus)


    Protein transduction using cell-penetrating peptides (CPPs) is useful for the delivery of large protein molecules, including some transcription factors. This method is safer than gene transfection methods with a viral vector because there is no risk of genomic integration of the exogenous DNA. Recently, this method was reported as a means for the induction of induced pluripotent stem (iPS) cells, directing the differentiation into specific cell types and supporting gene editing/correction. Furthermore, we developed a direct differentiation method to obtain a pancreatic lineage from mouse and human pluripotent stem cells via the protein transduction of three transcription factors, Pdxl, NeuroD, and MafA. Here, we discuss the possibility of using CPPs as a means of directing the differentiation of iPS cells and other stem cell technologies

    Original languageEnglish
    Pages (from-to)26667-26676
    Number of pages10
    JournalInternational journal of molecular sciences
    Issue number11
    Publication statusPublished - Nov 6 2015


    • Cell-penetrating peptide
    • Induced pluripotent stem cell
    • Pancreatic differentiation
    • Poly-arginine
    • Protein transduction

    ASJC Scopus subject areas

    • Catalysis
    • Molecular Biology
    • Spectroscopy
    • Computer Science Applications
    • Physical and Theoretical Chemistry
    • Organic Chemistry
    • Inorganic Chemistry


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