Cell-penetrating peptide as a means of directing the differentiation of induced-pluripotent stem cells

Taku Kaitsuka, Kazuhito Tomizawa

Research output: Contribution to journalReview article

9 Citations (Scopus)

Abstract

Protein transduction using cell-penetrating peptides (CPPs) is useful for the delivery of large protein molecules, including some transcription factors. This method is safer than gene transfection methods with a viral vector because there is no risk of genomic integration of the exogenous DNA. Recently, this method was reported as a means for the induction of induced pluripotent stem (iPS) cells, directing the differentiation into specific cell types and supporting gene editing/correction. Furthermore, we developed a direct differentiation method to obtain a pancreatic lineage from mouse and human pluripotent stem cells via the protein transduction of three transcription factors, Pdxl, NeuroD, and MafA. Here, we discuss the possibility of using CPPs as a means of directing the differentiation of iPS cells and other stem cell technologies

Original languageEnglish
Pages (from-to)26667-26676
Number of pages10
JournalInternational Journal of Molecular Sciences
Volume16
Issue number11
DOIs
Publication statusPublished - Nov 6 2015
Externally publishedYes

Fingerprint

Cell-Penetrating Peptides
Induced Pluripotent Stem Cells
stem cells
Stem cells
Peptides
peptides
Transcription factors
proteins
cells
Proteins
genes
Transcription Factors
Genes
editing
Pluripotent Stem Cells
Transfection
mice
Cell Differentiation
delivery
induction

Keywords

  • Cell-penetrating peptide
  • Induced pluripotent stem cell
  • Pancreatic differentiation
  • Poly-arginine
  • Protein transduction

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Spectroscopy
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Cell-penetrating peptide as a means of directing the differentiation of induced-pluripotent stem cells. / Kaitsuka, Taku; Tomizawa, Kazuhito.

In: International Journal of Molecular Sciences, Vol. 16, No. 11, 06.11.2015, p. 26667-26676.

Research output: Contribution to journalReview article

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