Cell-penetrating peptide as a means of directing the differentiation of induced-pluripotent stem cells

Taku Kaitsuka, Kazuhito Tomizawa

Research output: Contribution to journalReview article

10 Citations (Scopus)

Abstract

Protein transduction using cell-penetrating peptides (CPPs) is useful for the delivery of large protein molecules, including some transcription factors. This method is safer than gene transfection methods with a viral vector because there is no risk of genomic integration of the exogenous DNA. Recently, this method was reported as a means for the induction of induced pluripotent stem (iPS) cells, directing the differentiation into specific cell types and supporting gene editing/correction. Furthermore, we developed a direct differentiation method to obtain a pancreatic lineage from mouse and human pluripotent stem cells via the protein transduction of three transcription factors, Pdxl, NeuroD, and MafA. Here, we discuss the possibility of using CPPs as a means of directing the differentiation of iPS cells and other stem cell technologies

Original languageEnglish
Pages (from-to)26667-26676
Number of pages10
JournalInternational journal of molecular sciences
Volume16
Issue number11
DOIs
Publication statusPublished - Nov 6 2015

Keywords

  • Cell-penetrating peptide
  • Induced pluripotent stem cell
  • Pancreatic differentiation
  • Poly-arginine
  • Protein transduction

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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