Abstract
Stress-induced cell-lytic activity was found in tobacco BY-2 cells treated with various stresses. Among 14 stresses, an elicitor fraction isolated from Alternaria alternata showed the highest inducing activity. Cell-lytic activity increased for 72 h even in the control sample, treated with distilled water, and several isozymes of β-1,3-glucanases and chitinases were found to be involved in it. In contrast, cell-lytic activity in BY-2 cells treated with a fungal elicitor reached a higher level after 60 h. The principal enzymes specifically involved in this stress-induced portion are speculated to be basic β-1,3-glucanases. A class I β-1,3-glucanase gene (glu1) was found to be the specific gene for the stress-induced cell-lytic activity. Its expression became observable at 24 h, and the intensity reached a maximum at about 60-72 h. The glu1 was thus assigned as a late gene. Its role in the stress response is discussed in conjunction with earlier genes such as chitinases.
Original language | English |
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Pages (from-to) | 1265-1272 |
Number of pages | 8 |
Journal | Bioscience, Biotechnology and Biochemistry |
Volume | 68 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jun 2004 |
Externally published | Yes |
Keywords
- Alternaria alternata
- BY-2
- Cell-lytic activity
- Defense response
- β-1,3-glucanase
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry