Cell-lytic activity of tobacco BY-2 induced by a fungal elicitor from Alternaria alternata attributed to the expression of a class I β-1,3-glucanase gene

Tomonori Shinya, Shinobu Gondo, Hiroshi Iijima, Kazunari Hanai, Hideaki Matsuoka, Mikako Saito

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Stress-induced cell-lytic activity was found in tobacco BY-2 cells treated with various stresses. Among 14 stresses, an elicitor fraction isolated from Alternaria alternata showed the highest inducing activity. Cell-lytic activity increased for 72 h even in the control sample, treated with distilled water, and several isozymes of β-1,3-glucanases and chitinases were found to be involved in it. In contrast, cell-lytic activity in BY-2 cells treated with a fungal elicitor reached a higher level after 60 h. The principal enzymes specifically involved in this stress-induced portion are speculated to be basic β-1,3-glucanases. A class I β-1,3-glucanase gene (glu1) was found to be the specific gene for the stress-induced cell-lytic activity. Its expression became observable at 24 h, and the intensity reached a maximum at about 60-72 h. The glu1 was thus assigned as a late gene. Its role in the stress response is discussed in conjunction with earlier genes such as chitinases.

Original languageEnglish
Pages (from-to)1265-1272
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume68
Issue number6
DOIs
Publication statusPublished - Jun 1 2004
Externally publishedYes

Keywords

  • Alternaria alternata
  • BY-2
  • Cell-lytic activity
  • Defense response
  • β-1,3-glucanase

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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