@inbook{118343582edf4250879823b0dd9f6afc,
title = "Cell biological assays for measuring angiogenic activities of CCN proteins",
abstract = "Angiogenesis, the process of generating new blood vessels from an existing vasculature, is essential in normal developmental processes such as endochondral ossification and in numerous kinds of pathogenesis including tumor growth. A part from the action of angiogenic factor or antiangiogenic factor, it is still unknown at which stage of the angiogenic cascade these agents affect angiogenesis. Here, we describe methods for the use of connective tissue growth factor (CTGF/CCN2) and CCN2 neutralizing antibody in the currently used principal angiogenesis assays, including those in vitro ones for the proliferation, migration, adhesion, and tube formation of endothelial cells and in vivo assays such as those utilizing type I collagen implantation and the chick chorioallantoic membrane (CAM).",
keywords = "Adhesion, Cell proliferation, Migration, Tube formation",
author = "Tsuyoshi Shimo and Masaharu Takigawa",
note = "Funding Information: This study was supported by grants from the program Grants-in-aid for Scientific Research (B) [#JP24390415] and (B) [#JP15H05014] to M.T. from the Japan Society for the Promotion of Science and by the Wesco Scientific Promotion Foundation Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",
year = "2017",
doi = "10.1007/978-1-4939-6430-7_22",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "239--249",
booktitle = "Methods in Molecular Biology",
}