Cell biological assays for measuring angiogenic activities of CCN proteins

Tsuyoshi Shimo, Masaharu Takigawa

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Angiogenesis, the process of generating new blood vessels from an existing vasculature, is essential in normal developmental processes such as endochondral ossification and in numerous kinds of pathogenesis including tumor growth. A part from the action of angiogenic factor or antiangiogenic factor, it is still unknown at which stage of the angiogenic cascade these agents affect angiogenesis. Here, we describe methods for the use of connective tissue growth factor (CTGF/CCN2) and CCN2 neutralizing antibody in the currently used principal angiogenesis assays, including those in vitro ones for the proliferation, migration, adhesion, and tube formation of endothelial cells and in vivo assays such as those utilizing type I collagen implantation and the chick chorioallantoic membrane (CAM).

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages239-249
Number of pages11
Volume1489
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)10643745

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Keywords

  • Adhesion
  • Cell proliferation
  • Migration
  • Tube formation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Shimo, T., & Takigawa, M. (2017). Cell biological assays for measuring angiogenic activities of CCN proteins. In Methods in Molecular Biology (Vol. 1489, pp. 239-249). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_22