Abstract
Angiogenesis, the process of generating new blood vessels from an existing vasculature, is essential in normal developmental processes such as endochondral ossification and in numerous kinds of pathogenesis including tumor growth. A part from the action of angiogenic factor or antiangiogenic factor, it is still unknown at which stage of the angiogenic cascade these agents affect angiogenesis. Here, we describe methods for the use of connective tissue growth factor (CTGF/CCN2) and CCN2 neutralizing antibody in the currently used principal angiogenesis assays, including those in vitro ones for the proliferation, migration, adhesion, and tube formation of endothelial cells and in vivo assays such as those utilizing type I collagen implantation and the chick chorioallantoic membrane (CAM).
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 239-249 |
| Number of pages | 11 |
| Volume | 1489 |
| DOIs | |
| Publication status | Published - 2017 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1489 |
| ISSN (Print) | 10643745 |
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Keywords
- Adhesion
- Cell proliferation
- Migration
- Tube formation
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Cell biological assays for measuring angiogenic activities of CCN proteins. / Shimo, Tsuyoshi; Takigawa, Masaharu.
Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. p. 239-249 (Methods in Molecular Biology; Vol. 1489).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Cell biological assays for measuring angiogenic activities of CCN proteins
AU - Shimo, Tsuyoshi
AU - Takigawa, Masaharu
PY - 2017
Y1 - 2017
N2 - Angiogenesis, the process of generating new blood vessels from an existing vasculature, is essential in normal developmental processes such as endochondral ossification and in numerous kinds of pathogenesis including tumor growth. A part from the action of angiogenic factor or antiangiogenic factor, it is still unknown at which stage of the angiogenic cascade these agents affect angiogenesis. Here, we describe methods for the use of connective tissue growth factor (CTGF/CCN2) and CCN2 neutralizing antibody in the currently used principal angiogenesis assays, including those in vitro ones for the proliferation, migration, adhesion, and tube formation of endothelial cells and in vivo assays such as those utilizing type I collagen implantation and the chick chorioallantoic membrane (CAM).
AB - Angiogenesis, the process of generating new blood vessels from an existing vasculature, is essential in normal developmental processes such as endochondral ossification and in numerous kinds of pathogenesis including tumor growth. A part from the action of angiogenic factor or antiangiogenic factor, it is still unknown at which stage of the angiogenic cascade these agents affect angiogenesis. Here, we describe methods for the use of connective tissue growth factor (CTGF/CCN2) and CCN2 neutralizing antibody in the currently used principal angiogenesis assays, including those in vitro ones for the proliferation, migration, adhesion, and tube formation of endothelial cells and in vivo assays such as those utilizing type I collagen implantation and the chick chorioallantoic membrane (CAM).
KW - Adhesion
KW - Cell proliferation
KW - Migration
KW - Tube formation
UR - http://www.scopus.com/inward/record.url?scp=84991730064&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84991730064&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-6430-7_22
DO - 10.1007/978-1-4939-6430-7_22
M3 - Chapter
C2 - 27734381
AN - SCOPUS:84991730064
VL - 1489
T3 - Methods in Molecular Biology
SP - 239
EP - 249
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -