CEBiP is the major chitin oligomer-binding protein in rice and plays a main role in the perception of chitin oligomers

Yusuke Kouzai, Keisuke Nakajima, Masahiro Hayafune, Kenjirou Ozawa, Hanae Kaku, Naoto Shibuya, Eiichi Minami, Yoko Nishizawa

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

CEBiP, a plasma membrane-localized glycoprotein of rice, directly binds with chitin elicitors (CE), and has been identified as a receptor for CE by using CEBiP-RNAi rice cells. To further clarify the function of CEBiP, we produced CEBiP-disrupted rice plants by applying an efficient Agrobacterium-mediated gene-targeting system based on homologous recombination, which has recently been developed for rice. Homologous recombination occurred at the CEBiP locus in ~0.5 % of the positive/negative selected calli. In the self-pollinated next generation, it was confirmed that the first exon of CEBiP was replaced with the hygromycin selection cassette as designed, and that the expression of CEBiP was completely deficient in homozygous cebip lines. Affinity-labeling analysis using biotinylated N-acetylchitooctaose demonstrated that CEBiP is the major CE-binding protein in rice cultured cells and leaves, which was consistent with the result that the response to CE in cebip cells was greatly diminished. Nevertheless, we observed a significant decrease in disease resistance against Magnaporthe oryzae, the causal agent of rice blast disease, only when the cebip leaf sheaths were inoculated with a weakly virulent strain, suggesting that CE perception during the infection process of M. oryzae is limited. The response to peptidoglycan and lipopolysaccharides in cebip cells was not affected, strongly suggesting that CEBiP is a CE-specific receptor.

Original languageEnglish
Pages (from-to)519-528
Number of pages10
JournalPlant Molecular Biology
Volume84
Issue number4-5
DOIs
Publication statusPublished - Mar 2014
Externally publishedYes

Fingerprint

Chitin
chitin
binding proteins
Carrier Proteins
rice
Magnaporthe oryzae
blast disease
homologous recombination
Homologous Recombination
membrane glycoproteins
receptors
peptidoglycans
Magnaporthe
gene targeting
cells
Agrobacterium
cultured cells
lipopolysaccharides
Disease Resistance
exons

Keywords

  • CEBiP
  • Chitin receptor
  • Disease resistance
  • MAMPs
  • Oryza sativa L

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Genetics

Cite this

Kouzai, Y., Nakajima, K., Hayafune, M., Ozawa, K., Kaku, H., Shibuya, N., ... Nishizawa, Y. (2014). CEBiP is the major chitin oligomer-binding protein in rice and plays a main role in the perception of chitin oligomers. Plant Molecular Biology, 84(4-5), 519-528. https://doi.org/10.1007/s11103-013-0149-6

CEBiP is the major chitin oligomer-binding protein in rice and plays a main role in the perception of chitin oligomers. / Kouzai, Yusuke; Nakajima, Keisuke; Hayafune, Masahiro; Ozawa, Kenjirou; Kaku, Hanae; Shibuya, Naoto; Minami, Eiichi; Nishizawa, Yoko.

In: Plant Molecular Biology, Vol. 84, No. 4-5, 03.2014, p. 519-528.

Research output: Contribution to journalArticle

Kouzai, Y, Nakajima, K, Hayafune, M, Ozawa, K, Kaku, H, Shibuya, N, Minami, E & Nishizawa, Y 2014, 'CEBiP is the major chitin oligomer-binding protein in rice and plays a main role in the perception of chitin oligomers', Plant Molecular Biology, vol. 84, no. 4-5, pp. 519-528. https://doi.org/10.1007/s11103-013-0149-6
Kouzai, Yusuke ; Nakajima, Keisuke ; Hayafune, Masahiro ; Ozawa, Kenjirou ; Kaku, Hanae ; Shibuya, Naoto ; Minami, Eiichi ; Nishizawa, Yoko. / CEBiP is the major chitin oligomer-binding protein in rice and plays a main role in the perception of chitin oligomers. In: Plant Molecular Biology. 2014 ; Vol. 84, No. 4-5. pp. 519-528.
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