cDNA sequence encoding the 16-kDa proteolipid of chromaffin granules implies gene duplication in the evolution of H+-ATPases

M. Mandel, Y. Moriyama, J. D. Hulmes, Y. C E Pan, H. Nelson, N. Nelson

Research output: Contribution to journalArticle

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Abstract

Vacuolar H+-ATPases function in generating protonmotive force across the membranes of organelles connected with the vacuolar system of eukaryotic cells. This family of H+-ATPases is distinct from the two other families of H+-ATPases, the plasma membrane-type and the eubacterial-type. One of the subunits of the vacuolar H+-ATPase binds N,N'-dicyclohexylcarbodiimide (DCCD) and has been implicated in the proton-conducting activity of these enzymes. We have cloned and sequenced the gene encoding the DCCD-binding protein (proteolipid) of the H+-ATPase of bovine chromaffin granules. The gene encodes a highly hydrophobic protein of 15,849 Da. Hydropathy plots revealed four transmembrane segments, one of which contains a glutamic residue that is the likely candidate for the DCCD binding site. Sequence homology with the vacuolar proteolipid and with the proteolipids of eubacterial-type H+-ATPases was detected. The proteolipids from Escherichia coli, spinach chloroplasts, and yeast mitochondria matched better to the NH2-terminal part of the vacuolar protein. The proteolipids of bovine mitochondria and Neurospora mitochondria matched better to the COOH-terminal end of the vacuolar proteolipid. These findings suggest that the proteolipids of the vacuolar H+-ATPases were evolved in parallel with the eubacterial proteolipid, from a common ancestral gene that underwent gene duplication.

Original languageEnglish
Pages (from-to)5521-5524
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number15
Publication statusPublished - 1988
Externally publishedYes

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Chromaffin Granules
Proteolipids
Gene Duplication
Proton-Translocating ATPases
Complementary DNA
Vacuolar Proton-Translocating ATPases
Dicyclohexylcarbodiimide
Mitochondria
Genes
Neurospora
Spinacia oleracea
Eukaryotic Cells
Chloroplasts
Sequence Homology
Organelles
Protons
Carrier Proteins
Proteins
Yeasts
Binding Sites

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

cDNA sequence encoding the 16-kDa proteolipid of chromaffin granules implies gene duplication in the evolution of H+-ATPases. / Mandel, M.; Moriyama, Y.; Hulmes, J. D.; Pan, Y. C E; Nelson, H.; Nelson, N.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 15, 1988, p. 5521-5524.

Research output: Contribution to journalArticle

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