cDNA isolation and partial gene structure of the human α4(IV) collagen chain

Manabu Sugimoto, Toshitaka Oohashi, Hidekatsu Yoshioka, Nobuhiko Matsuo, Yoshifumi Ninomiya

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A novel collagen IV chain, α4(IV), has recently been identified in basement membranes. We describe part of the primary structure of the human α4(IV) polypeptide for the first time, which has been determined by cloning and sequencing of cDNAs encoding 241 amino acid residues of the COL domain and 231 residues of the NC1 domain. We also characterized a genomic DNA fragment containing 4 exons coding for the entire NCl domain. Among five known α chains of collagen IV, the α4(IV) chain is distinct from the other four chains. However, it is more similar to the α2 (IV) chain than to the α1(IV), α3(IV) and α5(IV) chains in terms of amino acid sequence homology, domain structure of polypeptides and exon/intron structure of the genes, suggesting the presence of two phylogenetically distinct subclasses of collagen IV α chains; one composed of α2 and α4 chains and the other of α1, α3 and α5 chains.

Original languageEnglish
Pages (from-to)122-128
Number of pages7
JournalFEBS Letters
Volume330
Issue number2
DOIs
Publication statusPublished - Sep 13 1993

Fingerprint

Collagen
Complementary DNA
Genes
Exons
Amino Acids
Amino Acid Sequence Homology
Peptides
Cloning
Basement Membrane
Introns
Organism Cloning
DNA

Keywords

  • Basement membrane
  • cDNA
  • Gene structure
  • mRNA
  • Type IV Collagen

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

cDNA isolation and partial gene structure of the human α4(IV) collagen chain. / Sugimoto, Manabu; Oohashi, Toshitaka; Yoshioka, Hidekatsu; Matsuo, Nobuhiko; Ninomiya, Yoshifumi.

In: FEBS Letters, Vol. 330, No. 2, 13.09.1993, p. 122-128.

Research output: Contribution to journalArticle

Sugimoto, Manabu ; Oohashi, Toshitaka ; Yoshioka, Hidekatsu ; Matsuo, Nobuhiko ; Ninomiya, Yoshifumi. / cDNA isolation and partial gene structure of the human α4(IV) collagen chain. In: FEBS Letters. 1993 ; Vol. 330, No. 2. pp. 122-128.
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