TY - JOUR
T1 - cDNA cloning of ACC synthase and ACC oxidase genes in cucumber fruit and their differential expression by wounding and auxin
AU - Shiomi, Shinjiro
AU - Yamamoto, Mikihiro
AU - Ono, Teiji
AU - Kakiuchi, Katsuya
AU - Nakamoto, Junichi
AU - Nakatsuka, Akira
AU - Kubo, Yasutaka
AU - Nakamura, Reinosuke
AU - Inaba, Akitsugu
AU - Imaseki, Hidemasa
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1998/9
Y1 - 1998/9
N2 - We cloned and sequenced three cDNA homologs of 1-aminocyclopropane-1-carboxylate (ACC) synthase (pCS-ACS1, pCS-ACS2 and pCS-ACS3) and two of ACC oxidase (pCS-ACO1 and pCS-ACO2) from cucumber (Cucumis sativus L.) fruit using RT-PCR (reverse transcription and polymerase chain reaction) and RACE (rapid amplification of cDNA ends) PCR. Conserved amino acid sequences reported for other plant tissues were found in all the cloned ACC synthase and ACC oxidase cDNAs. Among the ACC synthase and ACC oxidase cDNA clones, pCS-ACS1, pCS-ACS3, and pCS-ACO1 were highly homologous to ACC synthase and ACC oxidase genes cloned from other Cucurbitaceae family at both the nucleotide and the amino acid levels. Northern blot analysis showed that wounding induced the accumulation of CS-ACS1, CS-ACS2, and CS-ACO1 mRNAs, whereas IAA treatment induced expression of all the three ACC synthase genes and CS-ACO1. These expressions paralleled ethylene production in both treatments. Transcripts for CS-ACO2 were constitutively expressed and were not affected by wounding and IAA treatments. From the expression pattern and the sequence similarities of these genes, we conclude that CS-ACS1 could be a wound-inducible gene and that CS-ACS3 is an auxin-inducible one. These results strongly suggest that in cucumber fruit these genes are differentially expressed by wounding and auxin and are involved in the regulation of ethylene biosynthesis at the transcriptional level.
AB - We cloned and sequenced three cDNA homologs of 1-aminocyclopropane-1-carboxylate (ACC) synthase (pCS-ACS1, pCS-ACS2 and pCS-ACS3) and two of ACC oxidase (pCS-ACO1 and pCS-ACO2) from cucumber (Cucumis sativus L.) fruit using RT-PCR (reverse transcription and polymerase chain reaction) and RACE (rapid amplification of cDNA ends) PCR. Conserved amino acid sequences reported for other plant tissues were found in all the cloned ACC synthase and ACC oxidase cDNAs. Among the ACC synthase and ACC oxidase cDNA clones, pCS-ACS1, pCS-ACS3, and pCS-ACO1 were highly homologous to ACC synthase and ACC oxidase genes cloned from other Cucurbitaceae family at both the nucleotide and the amino acid levels. Northern blot analysis showed that wounding induced the accumulation of CS-ACS1, CS-ACS2, and CS-ACO1 mRNAs, whereas IAA treatment induced expression of all the three ACC synthase genes and CS-ACO1. These expressions paralleled ethylene production in both treatments. Transcripts for CS-ACO2 were constitutively expressed and were not affected by wounding and IAA treatments. From the expression pattern and the sequence similarities of these genes, we conclude that CS-ACS1 could be a wound-inducible gene and that CS-ACS3 is an auxin-inducible one. These results strongly suggest that in cucumber fruit these genes are differentially expressed by wounding and auxin and are involved in the regulation of ethylene biosynthesis at the transcriptional level.
KW - ACC oxidase (EC 1.4.3)
KW - ACC synthase (EC 4.4.1.14)
KW - Cucumis sativus
KW - IAA
KW - Wounding
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U2 - 10.2503/jjshs.67.685
DO - 10.2503/jjshs.67.685
M3 - Article
AN - SCOPUS:0032456210
VL - 67
SP - 685
EP - 692
JO - Horticulture Journal
JF - Horticulture Journal
SN - 2189-0102
IS - 5
ER -