cDNA cloning and nuclear localization of the circadian neuropeptide designated as pigment-dispersing factor PDF in the cricket Gryllus bimaculatus

Yoshiro Chuman, Ayami Matsushima, Seiji Sato, Kenji Tomioka, Yoshiya Tominaga, Ian A. Meinertzhagen, Yasuyuki Shimohigashi, Miki Shimohigashi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Pigment-dispersing factor (PDF) was recently reported to be a principal circadian neuromodulator involved in transmitting circadian rhythms of daily locomotion in insects. In Drosophila, PDF functions in some of the neurons expressing the clock genes period, timeless, Clock, and cycle, and those clock genes in turn regulate pdf gene expression. In the present study, we cloned a cDNA encoding PDF in the brain of a nocturnal insect, the cricket Gryllus bimaculatus, and found that an isolated clone (310 bp) codes for an extraordinarily short precursor protein with no definite signal sequence, but a nuclear localization signal (NLS)-like sequence instead. The cricket PDF exhibits high sequence identity (78-94%) and similarity (89-100%) to insect PDFs and also to crustacean β-PDH peptides. In the optic lobes of G. bimaculatus there are PDF-immunoreactive neurons in both the medulla and lamina neuropiles. Among the strongly immunoreactive lamina PDF neurons, on electron microscopy we identified cells exhibiting distinct staining that is not only cytoplasmic but also nuclear. When GFP-fused PDF precursor proteins were expressed in COS-7 cells, distinct translocation of the fusion protein into the nucleus was observed. This is the first finding of PDF peptide in the nucleus, which suggests a fundamental role of PDF peptide per se in the circadian clock system.

Original languageEnglish
Pages (from-to)895-903
Number of pages9
JournalJournal of Biochemistry
Volume131
Issue number6
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Gryllidae
Cloning
Neuropeptides
Pigments
Insects
Organism Cloning
Protein Precursors
Complementary DNA
Protein Sorting Signals
Neurons
Peptides
Clocks
Nuclear Localization Signals
Circadian Clocks
Neuropil
COS Cells
Protein Transport
Locomotion
Circadian Rhythm
Genes

Keywords

  • Circadian rhythm
  • Clock gene
  • Neuropeptide
  • Nuclear localization signal
  • Pigment-dispersing factor

ASJC Scopus subject areas

  • Biochemistry

Cite this

Chuman, Y., Matsushima, A., Sato, S., Tomioka, K., Tominaga, Y., Meinertzhagen, I. A., ... Shimohigashi, M. (2002). cDNA cloning and nuclear localization of the circadian neuropeptide designated as pigment-dispersing factor PDF in the cricket Gryllus bimaculatus. Journal of Biochemistry, 131(6), 895-903.

cDNA cloning and nuclear localization of the circadian neuropeptide designated as pigment-dispersing factor PDF in the cricket Gryllus bimaculatus. / Chuman, Yoshiro; Matsushima, Ayami; Sato, Seiji; Tomioka, Kenji; Tominaga, Yoshiya; Meinertzhagen, Ian A.; Shimohigashi, Yasuyuki; Shimohigashi, Miki.

In: Journal of Biochemistry, Vol. 131, No. 6, 2002, p. 895-903.

Research output: Contribution to journalArticle

Chuman, Y, Matsushima, A, Sato, S, Tomioka, K, Tominaga, Y, Meinertzhagen, IA, Shimohigashi, Y & Shimohigashi, M 2002, 'cDNA cloning and nuclear localization of the circadian neuropeptide designated as pigment-dispersing factor PDF in the cricket Gryllus bimaculatus', Journal of Biochemistry, vol. 131, no. 6, pp. 895-903.
Chuman, Yoshiro ; Matsushima, Ayami ; Sato, Seiji ; Tomioka, Kenji ; Tominaga, Yoshiya ; Meinertzhagen, Ian A. ; Shimohigashi, Yasuyuki ; Shimohigashi, Miki. / cDNA cloning and nuclear localization of the circadian neuropeptide designated as pigment-dispersing factor PDF in the cricket Gryllus bimaculatus. In: Journal of Biochemistry. 2002 ; Vol. 131, No. 6. pp. 895-903.
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