cDNA cloning and mRNA expression of Transformer 2 (Tra 2) in chicken embryo

Ichiro Yamamoto, Akira Tsukada, Noboru Saito, Kiyoshi Shimada

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We report the cloning of a chicken Transformer 2 (Tra 2) cDNA that encodes a protein of 289 amino acids which are 97.9% identical to those of mammalian splicing factor, Tra 2. Tra 2 mRNA was expressed in chicken embryonic tissues and was observed as a band of 1.5 kb by Northern blot analysis. Whole mount in situ hybridization showed an mRNA expression of Tra 2 in telencephalon, mandible, hyoid arch, wing and leg buds as early as day 3.5 of incubation. These results suggest that the Tra 2 gene may play a role in organogenesis in the chicken embryo.

Original languageEnglish
Pages (from-to)185-188
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1579
Issue number2-3
DOIs
Publication statusPublished - Dec 12 2002
Externally publishedYes

Keywords

  • Chicken
  • Embryo
  • Transformer 2 (Tra 2)
  • cDNA cloning

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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