cDNA cloning and mRNA expression of Transformer 2 (Tra 2) in chicken embryo

Ichiro Yamamoto; Akira Tsukada; Noboru Saito; Kiyoshi Shimada

doi:10.1016/S0167-4781(02)00511-0

cDNA cloning and mRNA expression of Transformer 2 (Tra 2) in chicken embryo

Ichiro Yamamoto, Akira Tsukada, Noboru Saito, Kiyoshi Shimada

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We report the cloning of a chicken Transformer 2 (Tra 2) cDNA that encodes a protein of 289 amino acids which are 97.9% identical to those of mammalian splicing factor, Tra 2. Tra 2 mRNA was expressed in chicken embryonic tissues and was observed as a band of 1.5 kb by Northern blot analysis. Whole mount in situ hybridization showed an mRNA expression of Tra 2 in telencephalon, mandible, hyoid arch, wing and leg buds as early as day 3.5 of incubation. These results suggest that the Tra 2 gene may play a role in organogenesis in the chicken embryo.

Original language	English
Pages (from-to)	185-188
Number of pages	4
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1579
Issue number	2-3
DOIs	https://doi.org/10.1016/S0167-4781(02)00511-0
Publication status	Published - Dec 12 2002
Externally published	Yes

Keywords

Chicken
Embryo
Transformer 2 (Tra 2)
cDNA cloning

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(02)00511-0

Cite this

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title = "cDNA cloning and mRNA expression of Transformer 2 (Tra 2) in chicken embryo",

abstract = "We report the cloning of a chicken Transformer 2 (Tra 2) cDNA that encodes a protein of 289 amino acids which are 97.9% identical to those of mammalian splicing factor, Tra 2. Tra 2 mRNA was expressed in chicken embryonic tissues and was observed as a band of 1.5 kb by Northern blot analysis. Whole mount in situ hybridization showed an mRNA expression of Tra 2 in telencephalon, mandible, hyoid arch, wing and leg buds as early as day 3.5 of incubation. These results suggest that the Tra 2 gene may play a role in organogenesis in the chicken embryo.",

keywords = "Chicken, Embryo, Transformer 2 (Tra 2), cDNA cloning",

author = "Ichiro Yamamoto and Akira Tsukada and Noboru Saito and Kiyoshi Shimada",

note = "Funding Information: We thank Dr. Craig A. Smith (Department of Paediatrics and Centre for Hormone Research, University of Melbourne, Royal Children's Hospital, Melbourne, Victoria, Australia) for teaching us the whole mount in situ hybridization technique. We also appreciate the Radioisotope Research Center of Nagoya University for use of the facilities. This study was supported by the Japanese Society for the Promotion of Science fellowship (01575) to I. Yamamoto.",

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doi = "10.1016/S0167-4781(02)00511-0",

language = "English",

volume = "1579",

pages = "185--188",

journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

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TY - JOUR

T1 - cDNA cloning and mRNA expression of Transformer 2 (Tra 2) in chicken embryo

AU - Yamamoto, Ichiro

AU - Tsukada, Akira

AU - Saito, Noboru

AU - Shimada, Kiyoshi

N1 - Funding Information: We thank Dr. Craig A. Smith (Department of Paediatrics and Centre for Hormone Research, University of Melbourne, Royal Children's Hospital, Melbourne, Victoria, Australia) for teaching us the whole mount in situ hybridization technique. We also appreciate the Radioisotope Research Center of Nagoya University for use of the facilities. This study was supported by the Japanese Society for the Promotion of Science fellowship (01575) to I. Yamamoto.

PY - 2002/12/12

Y1 - 2002/12/12

N2 - We report the cloning of a chicken Transformer 2 (Tra 2) cDNA that encodes a protein of 289 amino acids which are 97.9% identical to those of mammalian splicing factor, Tra 2. Tra 2 mRNA was expressed in chicken embryonic tissues and was observed as a band of 1.5 kb by Northern blot analysis. Whole mount in situ hybridization showed an mRNA expression of Tra 2 in telencephalon, mandible, hyoid arch, wing and leg buds as early as day 3.5 of incubation. These results suggest that the Tra 2 gene may play a role in organogenesis in the chicken embryo.

AB - We report the cloning of a chicken Transformer 2 (Tra 2) cDNA that encodes a protein of 289 amino acids which are 97.9% identical to those of mammalian splicing factor, Tra 2. Tra 2 mRNA was expressed in chicken embryonic tissues and was observed as a band of 1.5 kb by Northern blot analysis. Whole mount in situ hybridization showed an mRNA expression of Tra 2 in telencephalon, mandible, hyoid arch, wing and leg buds as early as day 3.5 of incubation. These results suggest that the Tra 2 gene may play a role in organogenesis in the chicken embryo.

KW - Chicken

KW - Embryo

KW - Transformer 2 (Tra 2)

KW - cDNA cloning

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DO - 10.1016/S0167-4781(02)00511-0

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AN - SCOPUS:0037069753

SN - 0167-4781

VL - 1579

SP - 185

EP - 188

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 2-3

ER -

cDNA cloning and mRNA expression of Transformer 2 (Tra 2) in chicken embryo

Abstract

Keywords

ASJC Scopus subject areas

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