cDNA Cloning and mRNA Expression of Estrogen Receptor α in Japanese Quail

Kouhei Ichikawa, Ichiro Yamamoto, Akira Tsukada, Noboru Saito, Kiyoshi Shimada

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The biological actions of estrogen are mediated via estrogen receptors (ER). Although chicken ER cDNA for subtypes α and β have been identified, only the β form of ER cDNA was cloned in quail. Hence, in this study the cDNA of ERα subtype was cloned and levels of mRNA expression were studied in different tissues in quail. Quail ERα cDNA containing the sequence containing the open reading frame (1770bp) encodes the predicted 589 amino acid residues and had high homology to chicken ERa (98.8%). Both RT-PCR and northern blot analyses indicate that relatively higher expression was observed in the liver, ovary, oviduct and testes. Relatively lower expression was detected in adrenal, cerebrum, hypothalamus, gizzard, heart, kidney and intestine by RT-PCR analysis. Conversely, low levels of expression of quail ERβ mRNA was detected in all the examined tissues. The results indicate that ERa subtype plays a predominant role for estrogenic actions in reproduction in quail.

Original languageEnglish
Pages (from-to)121-129
Number of pages9
JournalJournal of Poultry Science
Volume40
Issue number2
DOIs
Publication statusPublished - 2003
Externally publishedYes

Fingerprint

molecular cloning
quails
reverse transcriptase polymerase chain reaction
chickens
cerebrum
gizzard
hypothalamus
oviducts
Northern blotting
estrogens
open reading frames
Coturnix japonica
estrogen receptors
testes
intestines
heart
kidneys
liver
amino acids
tissues

Keywords

  • estrogen receptor α
  • expression
  • mRNA
  • northern blot analysis
  • quail
  • RT-PCR

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

cDNA Cloning and mRNA Expression of Estrogen Receptor α in Japanese Quail. / Ichikawa, Kouhei; Yamamoto, Ichiro; Tsukada, Akira; Saito, Noboru; Shimada, Kiyoshi.

In: Journal of Poultry Science, Vol. 40, No. 2, 2003, p. 121-129.

Research output: Contribution to journalArticle

Ichikawa, Kouhei ; Yamamoto, Ichiro ; Tsukada, Akira ; Saito, Noboru ; Shimada, Kiyoshi. / cDNA Cloning and mRNA Expression of Estrogen Receptor α in Japanese Quail. In: Journal of Poultry Science. 2003 ; Vol. 40, No. 2. pp. 121-129.
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