cDNA cloning and expression of mutant catalase from the hypocatalasemic mouse: Comparison with the acatalasemic mutant

Da Hong Wang, Ken Tsutsui, Kuniaki Sano, Noriyoshi Masuoka, Shohei Kira

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Mutant catalase cDNAs from the hypocatalasemic and acatalasemic mice were cloned and expressed in bacteria. A novel missense mutation, Asp (AAT) to Ser (AGT), was identified at amino acid position 439 of the hypocatalasemic catalase. Analysis of recombinant catalase mutants revealed that the mutation is responsible for the reduced activity of hypocatalasemic catalase and the unstable tetrameric structure of acatalasemic catalase was also suggested.

Original languageEnglish
Pages (from-to)217-220
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1522
Issue number3
DOIs
Publication statusPublished - Dec 30 2001

Keywords

  • Acatalasemic mouse
  • Catalase
  • Hypocatalasemic mouse
  • cDNA cloning
  • cDNA expression

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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