Abstract
Mutant catalase cDNAs from the hypocatalasemic and acatalasemic mice were cloned and expressed in bacteria. A novel missense mutation, Asp (AAT) to Ser (AGT), was identified at amino acid position 439 of the hypocatalasemic catalase. Analysis of recombinant catalase mutants revealed that the mutation is responsible for the reduced activity of hypocatalasemic catalase and the unstable tetrameric structure of acatalasemic catalase was also suggested.
| Original language | English |
|---|---|
| Pages (from-to) | 217-220 |
| Number of pages | 4 |
| Journal | BBA - Gene Structure and Expression |
| Volume | 1522 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Dec 30 2001 |
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Keywords
- Acatalasemic mouse
- Catalase
- cDNA cloning
- cDNA expression
- Hypocatalasemic mouse
ASJC Scopus subject areas
- Biochemistry
- Genetics
- Structural Biology
- Biophysics
Cite this
cDNA cloning and expression of mutant catalase from the hypocatalasemic mouse : Comparison with the acatalasemic mutant. / Wang, Da Hong; Tsutsui, Ken; Sano, Kuniaki; Masuoka, Noriyoshi; Kira, Shohei.
In: BBA - Gene Structure and Expression, Vol. 1522, No. 3, 30.12.2001, p. 217-220.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - cDNA cloning and expression of mutant catalase from the hypocatalasemic mouse
T2 - Comparison with the acatalasemic mutant
AU - Wang, Da Hong
AU - Tsutsui, Ken
AU - Sano, Kuniaki
AU - Masuoka, Noriyoshi
AU - Kira, Shohei
PY - 2001/12/30
Y1 - 2001/12/30
N2 - Mutant catalase cDNAs from the hypocatalasemic and acatalasemic mice were cloned and expressed in bacteria. A novel missense mutation, Asp (AAT) to Ser (AGT), was identified at amino acid position 439 of the hypocatalasemic catalase. Analysis of recombinant catalase mutants revealed that the mutation is responsible for the reduced activity of hypocatalasemic catalase and the unstable tetrameric structure of acatalasemic catalase was also suggested.
AB - Mutant catalase cDNAs from the hypocatalasemic and acatalasemic mice were cloned and expressed in bacteria. A novel missense mutation, Asp (AAT) to Ser (AGT), was identified at amino acid position 439 of the hypocatalasemic catalase. Analysis of recombinant catalase mutants revealed that the mutation is responsible for the reduced activity of hypocatalasemic catalase and the unstable tetrameric structure of acatalasemic catalase was also suggested.
KW - Acatalasemic mouse
KW - Catalase
KW - cDNA cloning
KW - cDNA expression
KW - Hypocatalasemic mouse
UR - http://www.scopus.com/inward/record.url?scp=0035977455&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035977455&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(01)00316-5
DO - 10.1016/S0167-4781(01)00316-5
M3 - Article
C2 - 11779637
AN - SCOPUS:0035977455
VL - 1522
SP - 217
EP - 220
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 3
ER -