cDNA cloning and expression of mutant catalase from the hypocatalasemic mouse: Comparison with the acatalasemic mutant

Da Hong Wang, Ken Tsutsui, Kuniaki Sano, Noriyoshi Masuoka, Shohei Kira

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Mutant catalase cDNAs from the hypocatalasemic and acatalasemic mice were cloned and expressed in bacteria. A novel missense mutation, Asp (AAT) to Ser (AGT), was identified at amino acid position 439 of the hypocatalasemic catalase. Analysis of recombinant catalase mutants revealed that the mutation is responsible for the reduced activity of hypocatalasemic catalase and the unstable tetrameric structure of acatalasemic catalase was also suggested.

Original languageEnglish
Pages (from-to)217-220
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1522
Issue number3
DOIs
Publication statusPublished - Dec 30 2001

Fingerprint

Cloning
Catalase
Organism Cloning
Complementary DNA
Missense Mutation
Bacteria
Amino Acids
Mutation

Keywords

  • Acatalasemic mouse
  • Catalase
  • cDNA cloning
  • cDNA expression
  • Hypocatalasemic mouse

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

cDNA cloning and expression of mutant catalase from the hypocatalasemic mouse : Comparison with the acatalasemic mutant. / Wang, Da Hong; Tsutsui, Ken; Sano, Kuniaki; Masuoka, Noriyoshi; Kira, Shohei.

In: BBA - Gene Structure and Expression, Vol. 1522, No. 3, 30.12.2001, p. 217-220.

Research output: Contribution to journalArticle

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