TY - JOUR
T1 - cDNA cloning and characterization of tobacco ABC transporter
T2 - NtPDR1 is a novel elicitor-responsive gene
AU - Sasabe, Michiko
AU - Toyoda, Kazuhiro
AU - Shiraishi, Tomonori
AU - Inagaki, Yoshishige
AU - Ichinose, Yuki
N1 - Funding Information:
We thank Prof. Sophien Kamoun, Ohio State University, for the gift of the inf1 gene and Dr. Fumiko Taguchi in our laboratory for the preparation of flagellin. This work was supported by a Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists, and also by Grants-in-Aid for Scientific Research on Priority Areas (No. 12052215) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2002/5/8
Y1 - 2002/5/8
N2 - We isolated an INF1 elicitin-inducible cDNA encoding a pleiotropic drug resistance (PDR)-type ATP-binding cassette (ABC) transporter homolog (NtPDR1) in suspension-cultured tobacco Bright Yellow-2 (BY-2) cells by application of differential display PCR. The NtPDR1 (Nicotiana tabacum PDR protein 1) gene also encodes a 162 kDa protein that includes two putative hydrophilic domains containing the ABC signature motif and two putative hydrophobic domains. Expression of the NtPDR1 gene was rapidly and strongly activated by treatment of BY-2 cells with INF1 elicitin. Further, treatment of BY-2 cells with flagellin, a bacterial proteinaceous hypersensitive reaction elicitor, or yeast extract, a general elicitor, also induced NtPDR1 gene expression. These results indicate that NtPDR1 may be involved in the general defense response in tobacco. This is the first report that microbial elicitors induce the expression of a plant ABC transporter gene.
AB - We isolated an INF1 elicitin-inducible cDNA encoding a pleiotropic drug resistance (PDR)-type ATP-binding cassette (ABC) transporter homolog (NtPDR1) in suspension-cultured tobacco Bright Yellow-2 (BY-2) cells by application of differential display PCR. The NtPDR1 (Nicotiana tabacum PDR protein 1) gene also encodes a 162 kDa protein that includes two putative hydrophilic domains containing the ABC signature motif and two putative hydrophobic domains. Expression of the NtPDR1 gene was rapidly and strongly activated by treatment of BY-2 cells with INF1 elicitin. Further, treatment of BY-2 cells with flagellin, a bacterial proteinaceous hypersensitive reaction elicitor, or yeast extract, a general elicitor, also induced NtPDR1 gene expression. These results indicate that NtPDR1 may be involved in the general defense response in tobacco. This is the first report that microbial elicitors induce the expression of a plant ABC transporter gene.
KW - ATP-binding cassette transporter
KW - Defense response
KW - Elicitin
KW - Elicitor
KW - Tobacco Bright Yellow-2
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U2 - 10.1016/S0014-5793(02)02697-2
DO - 10.1016/S0014-5793(02)02697-2
M3 - Article
C2 - 11997039
AN - SCOPUS:0037042223
VL - 518
SP - 164
EP - 168
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-3
ER -