TY - JOUR
T1 - cDNA array reveals mechanosensitive genes in chondrocytic cells under hydrostatic pressure
AU - Sironen, Reijo K.
AU - Karjalainen, Hannu M.
AU - Elo, Mika A.
AU - Kaarniranta, Kai
AU - Törrönen, Kari
AU - Takigawa, Masaharu
AU - Helminen, Heikki J.
AU - Lammi, Mikko J.
N1 - Funding Information:
This research report was supported by grants from the Finnish Medical Society, the Kuopio University Foundation, the Academy of Finland, the Sigrid Juselius Foundation and the Jenny and Antti Wihuri Foundation.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2002/8/19
Y1 - 2002/8/19
N2 - Hydrostatic pressure (HP) has a profound effect on cartilage metabolism in normal and pathological conditions, especially in weight-bearing areas of the skeletal system. As an important component of overall load, HP has been shown to affect the synthetic capacity and well-being of chondrocytes, depending on the mode, duration and magnitude of pressure. In this study we examined the effect of continuous HP on the gene expression profile of a chondrocytic cell line (HCS-2/8) using a cDNA array containing 588 well-characterized human genes under tight transcriptional control. A total of 51 affected genes were identified, many of them not previously associated with mechanical stimuli. Among the significantly up-regulated genes were immediate-early genes, and genes involved in heat-shock response (hsp70, hsp40, hsp27), and in growth arrest (GADD45, GADD153, p21Cip1/Waf1, tob). Markedly down-regulated genes included members of the Id family genes (dominant negative regulators of basic helix-loop-helix transcription factors), and cytoplasmic dynein light chain and apoptosis-related gene NIP3. These alterations in the expression profile induce a transient heat-shock gene response and activation of genes involved in growth arrest and cellular adaptation and/or differentiation.
AB - Hydrostatic pressure (HP) has a profound effect on cartilage metabolism in normal and pathological conditions, especially in weight-bearing areas of the skeletal system. As an important component of overall load, HP has been shown to affect the synthetic capacity and well-being of chondrocytes, depending on the mode, duration and magnitude of pressure. In this study we examined the effect of continuous HP on the gene expression profile of a chondrocytic cell line (HCS-2/8) using a cDNA array containing 588 well-characterized human genes under tight transcriptional control. A total of 51 affected genes were identified, many of them not previously associated with mechanical stimuli. Among the significantly up-regulated genes were immediate-early genes, and genes involved in heat-shock response (hsp70, hsp40, hsp27), and in growth arrest (GADD45, GADD153, p21Cip1/Waf1, tob). Markedly down-regulated genes included members of the Id family genes (dominant negative regulators of basic helix-loop-helix transcription factors), and cytoplasmic dynein light chain and apoptosis-related gene NIP3. These alterations in the expression profile induce a transient heat-shock gene response and activation of genes involved in growth arrest and cellular adaptation and/or differentiation.
KW - Chondrocytic cell
KW - Expression analysis
KW - Hydrostatic pressure
KW - mRNA stabilization
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U2 - 10.1016/S0167-4889(02)00247-1
DO - 10.1016/S0167-4889(02)00247-1
M3 - Article
C2 - 12183054
AN - SCOPUS:0037135713
VL - 1591
SP - 45
EP - 54
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
SN - 0167-4889
IS - 1-3
ER -