cDNA array reveals mechanosensitive genes in chondrocytic cells under hydrostatic pressure

Reijo K. Sironen, Hannu M. Karjalainen, Mika A. Elo, Kai Kaarniranta, Kari Törrönen, Masaharu Takigawa, Heikki J. Helminen, Mikko J. Lammi

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50 Citations (Scopus)

Abstract

Hydrostatic pressure (HP) has a profound effect on cartilage metabolism in normal and pathological conditions, especially in weight-bearing areas of the skeletal system. As an important component of overall load, HP has been shown to affect the synthetic capacity and well-being of chondrocytes, depending on the mode, duration and magnitude of pressure. In this study we examined the effect of continuous HP on the gene expression profile of a chondrocytic cell line (HCS-2/8) using a cDNA array containing 588 well-characterized human genes under tight transcriptional control. A total of 51 affected genes were identified, many of them not previously associated with mechanical stimuli. Among the significantly up-regulated genes were immediate-early genes, and genes involved in heat-shock response (hsp70, hsp40, hsp27), and in growth arrest (GADD45, GADD153, p21Cip1/Waf1, tob). Markedly down-regulated genes included members of the Id family genes (dominant negative regulators of basic helix-loop-helix transcription factors), and cytoplasmic dynein light chain and apoptosis-related gene NIP3. These alterations in the expression profile induce a transient heat-shock gene response and activation of genes involved in growth arrest and cellular adaptation and/or differentiation.

Original languageEnglish
Pages (from-to)45-54
Number of pages10
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1591
Issue number1-3
DOIs
Publication statusPublished - Aug 19 2002

Keywords

  • Chondrocytic cell
  • Expression analysis
  • Hydrostatic pressure
  • mRNA stabilization

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Sironen, R. K., Karjalainen, H. M., Elo, M. A., Kaarniranta, K., Törrönen, K., Takigawa, M., Helminen, H. J., & Lammi, M. J. (2002). cDNA array reveals mechanosensitive genes in chondrocytic cells under hydrostatic pressure. Biochimica et Biophysica Acta - Molecular Cell Research, 1591(1-3), 45-54. https://doi.org/10.1016/S0167-4889(02)00247-1