Abstract
Hydrostatic pressure (HP) has a profound effect on cartilage metabolism in normal and pathological conditions, especially in weight-bearing areas of the skeletal system. As an important component of overall load, HP has been shown to affect the synthetic capacity and well-being of chondrocytes, depending on the mode, duration and magnitude of pressure. In this study we examined the effect of continuous HP on the gene expression profile of a chondrocytic cell line (HCS-2/8) using a cDNA array containing 588 well-characterized human genes under tight transcriptional control. A total of 51 affected genes were identified, many of them not previously associated with mechanical stimuli. Among the significantly up-regulated genes were immediate-early genes, and genes involved in heat-shock response (hsp70, hsp40, hsp27), and in growth arrest (GADD45, GADD153, p21Cip1/Waf1, tob). Markedly down-regulated genes included members of the Id family genes (dominant negative regulators of basic helix-loop-helix transcription factors), and cytoplasmic dynein light chain and apoptosis-related gene NIP3. These alterations in the expression profile induce a transient heat-shock gene response and activation of genes involved in growth arrest and cellular adaptation and/or differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 45-54 |
| Number of pages | 10 |
| Journal | Biochimica et Biophysica Acta - Molecular Cell Research |
| Volume | 1591 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - Aug 19 2002 |
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Keywords
- Chondrocytic cell
- Expression analysis
- Hydrostatic pressure
- mRNA stabilization
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Biophysics
Cite this
cDNA array reveals mechanosensitive genes in chondrocytic cells under hydrostatic pressure. / Sironen, Reijo K.; Karjalainen, Hannu M.; Elo, Mika A.; Kaarniranta, Kai; Törrönen, Kari; Takigawa, Masaharu; Helminen, Heikki J.; Lammi, Mikko J.
In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1591, No. 1-3, 19.08.2002, p. 45-54.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - cDNA array reveals mechanosensitive genes in chondrocytic cells under hydrostatic pressure
AU - Sironen, Reijo K.
AU - Karjalainen, Hannu M.
AU - Elo, Mika A.
AU - Kaarniranta, Kai
AU - Törrönen, Kari
AU - Takigawa, Masaharu
AU - Helminen, Heikki J.
AU - Lammi, Mikko J.
PY - 2002/8/19
Y1 - 2002/8/19
N2 - Hydrostatic pressure (HP) has a profound effect on cartilage metabolism in normal and pathological conditions, especially in weight-bearing areas of the skeletal system. As an important component of overall load, HP has been shown to affect the synthetic capacity and well-being of chondrocytes, depending on the mode, duration and magnitude of pressure. In this study we examined the effect of continuous HP on the gene expression profile of a chondrocytic cell line (HCS-2/8) using a cDNA array containing 588 well-characterized human genes under tight transcriptional control. A total of 51 affected genes were identified, many of them not previously associated with mechanical stimuli. Among the significantly up-regulated genes were immediate-early genes, and genes involved in heat-shock response (hsp70, hsp40, hsp27), and in growth arrest (GADD45, GADD153, p21Cip1/Waf1, tob). Markedly down-regulated genes included members of the Id family genes (dominant negative regulators of basic helix-loop-helix transcription factors), and cytoplasmic dynein light chain and apoptosis-related gene NIP3. These alterations in the expression profile induce a transient heat-shock gene response and activation of genes involved in growth arrest and cellular adaptation and/or differentiation.
AB - Hydrostatic pressure (HP) has a profound effect on cartilage metabolism in normal and pathological conditions, especially in weight-bearing areas of the skeletal system. As an important component of overall load, HP has been shown to affect the synthetic capacity and well-being of chondrocytes, depending on the mode, duration and magnitude of pressure. In this study we examined the effect of continuous HP on the gene expression profile of a chondrocytic cell line (HCS-2/8) using a cDNA array containing 588 well-characterized human genes under tight transcriptional control. A total of 51 affected genes were identified, many of them not previously associated with mechanical stimuli. Among the significantly up-regulated genes were immediate-early genes, and genes involved in heat-shock response (hsp70, hsp40, hsp27), and in growth arrest (GADD45, GADD153, p21Cip1/Waf1, tob). Markedly down-regulated genes included members of the Id family genes (dominant negative regulators of basic helix-loop-helix transcription factors), and cytoplasmic dynein light chain and apoptosis-related gene NIP3. These alterations in the expression profile induce a transient heat-shock gene response and activation of genes involved in growth arrest and cellular adaptation and/or differentiation.
KW - Chondrocytic cell
KW - Expression analysis
KW - Hydrostatic pressure
KW - mRNA stabilization
UR - http://www.scopus.com/inward/record.url?scp=0037135713&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037135713&partnerID=8YFLogxK
U2 - 10.1016/S0167-4889(02)00247-1
DO - 10.1016/S0167-4889(02)00247-1
M3 - Article
C2 - 12183054
AN - SCOPUS:0037135713
VL - 1591
SP - 45
EP - 54
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
SN - 0167-4889
IS - 1-3
ER -