Abstract
Background: CD80 (B7-1) and CD86 (B7-2) play an important role in antigen presentation to effector cells. Recent studies have demonstrated that these costimulatory molecules are also expressed on activated T cells. However, the functional role of CD80 and CD86 expressed on allergen-specific T cells in atopic diseases has not yet been clarified. Objective: We sought to determine the functional role of CD80 and CD86 expressed on allergen- specific T cells in atopic diseases. Methods: We assayed the expression of CD80 and CD86 on allergen-specific T-cell lines from patients with perennial allergic rhinitis stimulated by Dermatophagoides farinae-crude (Der f-c) antigen, 1 of the major allergens causing house dust mite allergy. T-cell proliferation induced by Der f-c-specific TT cell interactions was measured, and the role of CD80 and CD86 in this proliferation was examined. In addition, we compared the proportion of CD45RO+CD86+ T cells in primary culture of PBMCs stimulated by Der f-c antigen between patients with perennial allergic rhinitis and control subjects. Results: On T-cell activation, CD86 antigen was upregulated earlier than CD80. Both CD80 and CD86 expressed on Der f-c-specific T cells could provide costimulatory signals to induce allergen-specific T-cell proliferation that was partially inhibitable by both anti-CD80 and anti-CD86 mAbs. The proportion of CD45RO+CD86+ T cells in primary culture from atopic patients was significantly higher than that from control subjects. Conclusion: These results suggest that costimulatory molecules, such as CD80 and CD86, expressed on allergen-specific T cells may be involved in the amplification of allergen-specific immune responses through T-T cell interactions in atopic diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 222-227 |
| Number of pages | 6 |
| Journal | Journal of Allergy and Clinical Immunology |
| Volume | 104 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1999 |
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Keywords
- Allergen-specific T cell
- Atopic disease
- CD80
- CD86
- T-T cell interactions
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
CD80 (B7-1) and CD86 (B7-2) antigens on house dust mite-specific T cells in atopic disease function through T-T cell interactions. / Nakada, Michihiro; Nishizaki, Kazunori; Yoshino, Tadashi; Okano, Mitsuhiro; Yamamoto, Takayoshi; Masuda, Yu; Ohta, Nobuo; Akagi, Tadaatsu.
In: Journal of Allergy and Clinical Immunology, Vol. 104, No. 1, 1999, p. 222-227.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CD80 (B7-1) and CD86 (B7-2) antigens on house dust mite-specific T cells in atopic disease function through T-T cell interactions
AU - Nakada, Michihiro
AU - Nishizaki, Kazunori
AU - Yoshino, Tadashi
AU - Okano, Mitsuhiro
AU - Yamamoto, Takayoshi
AU - Masuda, Yu
AU - Ohta, Nobuo
AU - Akagi, Tadaatsu
PY - 1999
Y1 - 1999
N2 - Background: CD80 (B7-1) and CD86 (B7-2) play an important role in antigen presentation to effector cells. Recent studies have demonstrated that these costimulatory molecules are also expressed on activated T cells. However, the functional role of CD80 and CD86 expressed on allergen-specific T cells in atopic diseases has not yet been clarified. Objective: We sought to determine the functional role of CD80 and CD86 expressed on allergen- specific T cells in atopic diseases. Methods: We assayed the expression of CD80 and CD86 on allergen-specific T-cell lines from patients with perennial allergic rhinitis stimulated by Dermatophagoides farinae-crude (Der f-c) antigen, 1 of the major allergens causing house dust mite allergy. T-cell proliferation induced by Der f-c-specific TT cell interactions was measured, and the role of CD80 and CD86 in this proliferation was examined. In addition, we compared the proportion of CD45RO+CD86+ T cells in primary culture of PBMCs stimulated by Der f-c antigen between patients with perennial allergic rhinitis and control subjects. Results: On T-cell activation, CD86 antigen was upregulated earlier than CD80. Both CD80 and CD86 expressed on Der f-c-specific T cells could provide costimulatory signals to induce allergen-specific T-cell proliferation that was partially inhibitable by both anti-CD80 and anti-CD86 mAbs. The proportion of CD45RO+CD86+ T cells in primary culture from atopic patients was significantly higher than that from control subjects. Conclusion: These results suggest that costimulatory molecules, such as CD80 and CD86, expressed on allergen-specific T cells may be involved in the amplification of allergen-specific immune responses through T-T cell interactions in atopic diseases.
AB - Background: CD80 (B7-1) and CD86 (B7-2) play an important role in antigen presentation to effector cells. Recent studies have demonstrated that these costimulatory molecules are also expressed on activated T cells. However, the functional role of CD80 and CD86 expressed on allergen-specific T cells in atopic diseases has not yet been clarified. Objective: We sought to determine the functional role of CD80 and CD86 expressed on allergen- specific T cells in atopic diseases. Methods: We assayed the expression of CD80 and CD86 on allergen-specific T-cell lines from patients with perennial allergic rhinitis stimulated by Dermatophagoides farinae-crude (Der f-c) antigen, 1 of the major allergens causing house dust mite allergy. T-cell proliferation induced by Der f-c-specific TT cell interactions was measured, and the role of CD80 and CD86 in this proliferation was examined. In addition, we compared the proportion of CD45RO+CD86+ T cells in primary culture of PBMCs stimulated by Der f-c antigen between patients with perennial allergic rhinitis and control subjects. Results: On T-cell activation, CD86 antigen was upregulated earlier than CD80. Both CD80 and CD86 expressed on Der f-c-specific T cells could provide costimulatory signals to induce allergen-specific T-cell proliferation that was partially inhibitable by both anti-CD80 and anti-CD86 mAbs. The proportion of CD45RO+CD86+ T cells in primary culture from atopic patients was significantly higher than that from control subjects. Conclusion: These results suggest that costimulatory molecules, such as CD80 and CD86, expressed on allergen-specific T cells may be involved in the amplification of allergen-specific immune responses through T-T cell interactions in atopic diseases.
KW - Allergen-specific T cell
KW - Atopic disease
KW - CD80
KW - CD86
KW - T-T cell interactions
UR - http://www.scopus.com/inward/record.url?scp=0032866885&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032866885&partnerID=8YFLogxK
U2 - 10.1016/S0091-6749(99)70139-9
DO - 10.1016/S0091-6749(99)70139-9
M3 - Article
C2 - 10400865
AN - SCOPUS:0032866885
VL - 104
SP - 222
EP - 227
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
SN - 0091-6749
IS - 1
ER -