CD80 (B7-1) and CD86 (B7-2) antigens on house dust mite-specific T cells in atopic disease function through T-T cell interactions

Michihiro Nakada, Kazunori Nishizaki, Tadashi Yoshino, Mitsuhiro Okano, Takayoshi Yamamoto, Yu Masuda, Nobuo Ohta, Tadaatsu Akagi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: CD80 (B7-1) and CD86 (B7-2) play an important role in antigen presentation to effector cells. Recent studies have demonstrated that these costimulatory molecules are also expressed on activated T cells. However, the functional role of CD80 and CD86 expressed on allergen-specific T cells in atopic diseases has not yet been clarified. Objective: We sought to determine the functional role of CD80 and CD86 expressed on allergen- specific T cells in atopic diseases. Methods: We assayed the expression of CD80 and CD86 on allergen-specific T-cell lines from patients with perennial allergic rhinitis stimulated by Dermatophagoides farinae-crude (Der f-c) antigen, 1 of the major allergens causing house dust mite allergy. T-cell proliferation induced by Der f-c-specific TT cell interactions was measured, and the role of CD80 and CD86 in this proliferation was examined. In addition, we compared the proportion of CD45RO+CD86+ T cells in primary culture of PBMCs stimulated by Der f-c antigen between patients with perennial allergic rhinitis and control subjects. Results: On T-cell activation, CD86 antigen was upregulated earlier than CD80. Both CD80 and CD86 expressed on Der f-c-specific T cells could provide costimulatory signals to induce allergen-specific T-cell proliferation that was partially inhibitable by both anti-CD80 and anti-CD86 mAbs. The proportion of CD45RO+CD86+ T cells in primary culture from atopic patients was significantly higher than that from control subjects. Conclusion: These results suggest that costimulatory molecules, such as CD80 and CD86, expressed on allergen-specific T cells may be involved in the amplification of allergen-specific immune responses through T-T cell interactions in atopic diseases.

Original languageEnglish
Pages (from-to)222-227
Number of pages6
JournalJournal of Allergy and Clinical Immunology
Volume104
Issue number1
DOIs
Publication statusPublished - Jan 1 1999

Keywords

  • Allergen-specific T cell
  • Atopic disease
  • CD80
  • CD86
  • T-T cell interactions

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'CD80 (B7-1) and CD86 (B7-2) antigens on house dust mite-specific T cells in atopic disease function through T-T cell interactions'. Together they form a unique fingerprint.

  • Cite this