CD80 (B7-1) and CD86 (B7-2) antigens on house dust mite-specific T cells in atopic disease function through T-T cell interactions

Michihiro Nakada, Kazunori Nishizaki, Tadashi Yoshino, Mitsuhiro Okano, Takayoshi Yamamoto, Yu Masuda, Nobuo Ohta, Tadaatsu Akagi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: CD80 (B7-1) and CD86 (B7-2) play an important role in antigen presentation to effector cells. Recent studies have demonstrated that these costimulatory molecules are also expressed on activated T cells. However, the functional role of CD80 and CD86 expressed on allergen-specific T cells in atopic diseases has not yet been clarified. Objective: We sought to determine the functional role of CD80 and CD86 expressed on allergen- specific T cells in atopic diseases. Methods: We assayed the expression of CD80 and CD86 on allergen-specific T-cell lines from patients with perennial allergic rhinitis stimulated by Dermatophagoides farinae-crude (Der f-c) antigen, 1 of the major allergens causing house dust mite allergy. T-cell proliferation induced by Der f-c-specific TT cell interactions was measured, and the role of CD80 and CD86 in this proliferation was examined. In addition, we compared the proportion of CD45RO+CD86+ T cells in primary culture of PBMCs stimulated by Der f-c antigen between patients with perennial allergic rhinitis and control subjects. Results: On T-cell activation, CD86 antigen was upregulated earlier than CD80. Both CD80 and CD86 expressed on Der f-c-specific T cells could provide costimulatory signals to induce allergen-specific T-cell proliferation that was partially inhibitable by both anti-CD80 and anti-CD86 mAbs. The proportion of CD45RO+CD86+ T cells in primary culture from atopic patients was significantly higher than that from control subjects. Conclusion: These results suggest that costimulatory molecules, such as CD80 and CD86, expressed on allergen-specific T cells may be involved in the amplification of allergen-specific immune responses through T-T cell interactions in atopic diseases.

Original languageEnglish
Pages (from-to)222-227
Number of pages6
JournalJournal of Allergy and Clinical Immunology
Volume104
Issue number1
DOIs
Publication statusPublished - 1999

Fingerprint

CD86 Antigens
Pyroglyphidae
Cell Communication
T-Lymphocytes
Allergens
Dermatophagoides Antigens
Rhinitis, Allergic, Perennial
Dermatophagoides farinae
CD27 Antigens
Cell Proliferation
Antigen Presentation

Keywords

  • Allergen-specific T cell
  • Atopic disease
  • CD80
  • CD86
  • T-T cell interactions

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

CD80 (B7-1) and CD86 (B7-2) antigens on house dust mite-specific T cells in atopic disease function through T-T cell interactions. / Nakada, Michihiro; Nishizaki, Kazunori; Yoshino, Tadashi; Okano, Mitsuhiro; Yamamoto, Takayoshi; Masuda, Yu; Ohta, Nobuo; Akagi, Tadaatsu.

In: Journal of Allergy and Clinical Immunology, Vol. 104, No. 1, 1999, p. 222-227.

Research output: Contribution to journalArticle

Nakada, Michihiro ; Nishizaki, Kazunori ; Yoshino, Tadashi ; Okano, Mitsuhiro ; Yamamoto, Takayoshi ; Masuda, Yu ; Ohta, Nobuo ; Akagi, Tadaatsu. / CD80 (B7-1) and CD86 (B7-2) antigens on house dust mite-specific T cells in atopic disease function through T-T cell interactions. In: Journal of Allergy and Clinical Immunology. 1999 ; Vol. 104, No. 1. pp. 222-227.
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T1 - CD80 (B7-1) and CD86 (B7-2) antigens on house dust mite-specific T cells in atopic disease function through T-T cell interactions

AU - Nakada, Michihiro

AU - Nishizaki, Kazunori

AU - Yoshino, Tadashi

AU - Okano, Mitsuhiro

AU - Yamamoto, Takayoshi

AU - Masuda, Yu

AU - Ohta, Nobuo

AU - Akagi, Tadaatsu

PY - 1999

Y1 - 1999

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AB - Background: CD80 (B7-1) and CD86 (B7-2) play an important role in antigen presentation to effector cells. Recent studies have demonstrated that these costimulatory molecules are also expressed on activated T cells. However, the functional role of CD80 and CD86 expressed on allergen-specific T cells in atopic diseases has not yet been clarified. Objective: We sought to determine the functional role of CD80 and CD86 expressed on allergen- specific T cells in atopic diseases. Methods: We assayed the expression of CD80 and CD86 on allergen-specific T-cell lines from patients with perennial allergic rhinitis stimulated by Dermatophagoides farinae-crude (Der f-c) antigen, 1 of the major allergens causing house dust mite allergy. T-cell proliferation induced by Der f-c-specific TT cell interactions was measured, and the role of CD80 and CD86 in this proliferation was examined. In addition, we compared the proportion of CD45RO+CD86+ T cells in primary culture of PBMCs stimulated by Der f-c antigen between patients with perennial allergic rhinitis and control subjects. Results: On T-cell activation, CD86 antigen was upregulated earlier than CD80. Both CD80 and CD86 expressed on Der f-c-specific T cells could provide costimulatory signals to induce allergen-specific T-cell proliferation that was partially inhibitable by both anti-CD80 and anti-CD86 mAbs. The proportion of CD45RO+CD86+ T cells in primary culture from atopic patients was significantly higher than that from control subjects. Conclusion: These results suggest that costimulatory molecules, such as CD80 and CD86, expressed on allergen-specific T cells may be involved in the amplification of allergen-specific immune responses through T-T cell interactions in atopic diseases.

KW - Allergen-specific T cell

KW - Atopic disease

KW - CD80

KW - CD86

KW - T-T cell interactions

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