TY - JOUR
T1 - CD4+ cells are indispensable for ulcer development in murine cutaneous leishmaniasis
AU - Terabe, Masaki
AU - Kuramochi, Takashi
AU - Ito, Mamoru
AU - Hatabu, Toshimitsu
AU - Sanjoba, Chizu
AU - Chang, Kwang Poo
AU - Onodera, Takashi
AU - Matsumoto, Yoshitsugu
PY - 2000
Y1 - 2000
N2 - One of the most characteristic clinical features in cutaneous leishmaniasis is the development of nodules followed by ulcerations at the site of infection. Leishmania amazonensis-infected mice show similar ulcerative lesions. Leishmania-infected severe combined immunodeficiency (SCID) mice, however, have been shown to develop nonulcerative nodules. In the present study, the roles of T cells in ulceration were examined using SCID mice in cell reconstitution experiments. After development of nonulcerative nodules, SCID mice were inoculated with splenocytes from either Leishmania-infected or naive immunocompetent mice, resulting in ulceration in all mice. When naive splenocytes were depleted of CD4+, CD8+, or B220+ cell populations and the remaining cells were injected into Leishmania- infected SCID mice after the development of nodules, only SCID mice inoculated with splenocytes depleted of CD4+ cells did not show ulceration. The evidence obtained in this study clearly shows that the CD4+ cell population is indispensable for ulceration in leishmaniasis lesions of SCID mice.
AB - One of the most characteristic clinical features in cutaneous leishmaniasis is the development of nodules followed by ulcerations at the site of infection. Leishmania amazonensis-infected mice show similar ulcerative lesions. Leishmania-infected severe combined immunodeficiency (SCID) mice, however, have been shown to develop nonulcerative nodules. In the present study, the roles of T cells in ulceration were examined using SCID mice in cell reconstitution experiments. After development of nonulcerative nodules, SCID mice were inoculated with splenocytes from either Leishmania-infected or naive immunocompetent mice, resulting in ulceration in all mice. When naive splenocytes were depleted of CD4+, CD8+, or B220+ cell populations and the remaining cells were injected into Leishmania- infected SCID mice after the development of nodules, only SCID mice inoculated with splenocytes depleted of CD4+ cells did not show ulceration. The evidence obtained in this study clearly shows that the CD4+ cell population is indispensable for ulceration in leishmaniasis lesions of SCID mice.
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U2 - 10.1128/IAI.68.8.4574-4577.2000
DO - 10.1128/IAI.68.8.4574-4577.2000
M3 - Article
C2 - 10899857
AN - SCOPUS:0033914835
VL - 68
SP - 4574
EP - 4577
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 8
ER -