CD45 is Required for CD40-induced Inhibition of DNA Synthesis and Regulation of c-Jun NH2-terminal Kinase and p38 in BAL-17 B Cells

Yutaka Arimura, Mami Ogimoto, Katsuyuki Mitomo, Tatsuo Katagiri, Ken-ichi Yamamoto, Sinisa Volarevic, Kazuya Mizuno, Hidetaka Yakura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Stimulation of B cell antigen receptor (BCR) may induce proliferation, differentiation, or apoptosis, depending upon the maturational stage of the cell and the presence or absence of signals transmitted via coreceptors. One such signal is delivered via CD40; for instance, ligation of CD40 rescues B cells from BCR-induced apoptosis. Here we show that, in contrast to WEHI-231 cells, CD40 ligation did not reverse BCR-induced growth inhibition in the BAL-17 mature B cell line and CD40 ligation itself inhibited proliferation. This inhibitory signaling was not observed in CD45-deficient cells. Further analyses demonstrate that transfection of dominant-negative form of SEK1 or treatment with SB203580 strongly reduced CD40-induced inhibition of BAL-17 proliferation, suggesting a requirement for c-Jun NH2-terminal kinase and p38 in CD40-induced inhibition of proliferation. Interestingly, CD40-initiated activation of c-Jun NH2-terminal kinase and p38 was enhanced and sustained in CD45-deficient cells, and these phenotypes were reversed by transfecting CD45 gene. However, CD40-mediated induction of cell surface molecules was not affected in CD45-deficient cells. Taken collectively, these results suggest that CD45 exerts a decisive effect on selective sets of CD40-mediated signaling pathways, dictating B cell fate.

Original languageEnglish
Pages (from-to)8550-8556
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number11
DOIs
Publication statusPublished - Mar 16 2001
Externally publishedYes

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Dimercaprol
JNK Mitogen-Activated Protein Kinases
B-Cell Antigen Receptors
B-Lymphocytes
Cells
DNA
Ligation
Apoptosis
Genes
Chemical activation
Molecules
Transfection
Phenotype
Cell Line
Growth

ASJC Scopus subject areas

  • Biochemistry

Cite this

CD45 is Required for CD40-induced Inhibition of DNA Synthesis and Regulation of c-Jun NH2-terminal Kinase and p38 in BAL-17 B Cells. / Arimura, Yutaka; Ogimoto, Mami; Mitomo, Katsuyuki; Katagiri, Tatsuo; Yamamoto, Ken-ichi; Volarevic, Sinisa; Mizuno, Kazuya; Yakura, Hidetaka.

In: Journal of Biological Chemistry, Vol. 276, No. 11, 16.03.2001, p. 8550-8556.

Research output: Contribution to journalArticle

Arimura, Yutaka ; Ogimoto, Mami ; Mitomo, Katsuyuki ; Katagiri, Tatsuo ; Yamamoto, Ken-ichi ; Volarevic, Sinisa ; Mizuno, Kazuya ; Yakura, Hidetaka. / CD45 is Required for CD40-induced Inhibition of DNA Synthesis and Regulation of c-Jun NH2-terminal Kinase and p38 in BAL-17 B Cells. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 11. pp. 8550-8556.
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