CD41 is a reliable identification and activation marker for murine basophils in the steady state and during helminth and malarial infections

Nadja Bakocevic, Carla Claser, Soichiro Yoshikawa, Leigh Ann Jones, Samantha Chew, Chi Ching Goh, Benoit Malleret, Anis Larbi, Florent Ginhoux, Maria Curotto de Lafaille, Hajime Karasuyama, Laurent Renia, Lai Guan Ng

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Basophils, a rare leukocyte population in peripheral circulation, are conventionally identified as CD45intCD49b+FcεRI+ cells. Here, we show that basophils from blood and several organs of naïve wild-type mice express CD41, the α subunit of αIIbβ3 integrin. CD41 expression on basophils is upregulated after in vivo IL-3 treatment and during infection with Nippostrongylus brasiliensis (Nb). Moreover, CD41 can be used as a reliable marker for basophils, circumventing technical difficulties associated with FcεRI for basophil identification in a Nb infection model. In vitro anti-IgE cross-linking and IL-3 basophil stimulation showed that CD41 upregulation positively correlates with augmented surface expression of CD200R and increased production of IL-4/IL-13, indicating that CD41 is a basophil activation marker. Furthermore, we found that infection with Plasmodium yoelii 17X (Py17x) induced a profound basophilia and using Mcpt8DTR reporter mice as a basophil-specific depletion model, we verified that CD41 can be used as a marker to track basophils in the steady state and during infection. During malarial infection, CD41 expression on basophils is negatively regulated by IFN-γ and positively correlates with increased basophil IL-4 production. In conclusion, we provide evidence that CD41 can be used as both an identification and activation marker for basophils during homeostasis and immune challenge.

Original languageEnglish
Pages (from-to)1823-1834
Number of pages12
JournalEuropean Journal of Immunology
Volume44
Issue number6
DOIs
Publication statusPublished - Jun 2014
Externally publishedYes

Fingerprint

Basophils
Helminths
Infection
Nippostrongylus
Interleukin-3
Interleukin-4
Plasmodium yoelii
Interleukin-13
Integrins
Leukocytes
Homeostasis
Up-Regulation

Keywords

  • Basophils
  • CD41
  • Helminths
  • Malaria

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

CD41 is a reliable identification and activation marker for murine basophils in the steady state and during helminth and malarial infections. / Bakocevic, Nadja; Claser, Carla; Yoshikawa, Soichiro; Jones, Leigh Ann; Chew, Samantha; Goh, Chi Ching; Malleret, Benoit; Larbi, Anis; Ginhoux, Florent; de Lafaille, Maria Curotto; Karasuyama, Hajime; Renia, Laurent; Ng, Lai Guan.

In: European Journal of Immunology, Vol. 44, No. 6, 06.2014, p. 1823-1834.

Research output: Contribution to journalArticle

Bakocevic, N, Claser, C, Yoshikawa, S, Jones, LA, Chew, S, Goh, CC, Malleret, B, Larbi, A, Ginhoux, F, de Lafaille, MC, Karasuyama, H, Renia, L & Ng, LG 2014, 'CD41 is a reliable identification and activation marker for murine basophils in the steady state and during helminth and malarial infections', European Journal of Immunology, vol. 44, no. 6, pp. 1823-1834. https://doi.org/10.1002/eji.201344254
Bakocevic, Nadja ; Claser, Carla ; Yoshikawa, Soichiro ; Jones, Leigh Ann ; Chew, Samantha ; Goh, Chi Ching ; Malleret, Benoit ; Larbi, Anis ; Ginhoux, Florent ; de Lafaille, Maria Curotto ; Karasuyama, Hajime ; Renia, Laurent ; Ng, Lai Guan. / CD41 is a reliable identification and activation marker for murine basophils in the steady state and during helminth and malarial infections. In: European Journal of Immunology. 2014 ; Vol. 44, No. 6. pp. 1823-1834.
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AU - Jones, Leigh Ann

AU - Chew, Samantha

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AU - de Lafaille, Maria Curotto

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