CD4+ cells are indispensable for ulcer development in murine cutaneous leishmaniasis

Masaki Terabe, Takashi Kuramochi, Mamoru Ito, Toshimitsu Hatabu, Chizu Sanjoba, Kwang Poo Chang, Takashi Onodera, Yoshitsugu Matsumoto

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

One of the most characteristic clinical features in cutaneous leishmaniasis is the development of nodules followed by ulcerations at the site of infection. Leishmania amazonensis-infected mice show similar ulcerative lesions. Leishmania-infected severe combined immunodeficiency (SCID) mice, however, have been shown to develop nonulcerative nodules. In the present study, the roles of T cells in ulceration were examined using SCID mice in cell reconstitution experiments. After development of nonulcerative nodules, SCID mice were inoculated with splenocytes from either Leishmania-infected or naive immunocompetent mice, resulting in ulceration in all mice. When naive splenocytes were depleted of CD4+, CD8+, or B220+ cell populations and the remaining cells were injected into Leishmania- infected SCID mice after the development of nodules, only SCID mice inoculated with splenocytes depleted of CD4+ cells did not show ulceration. The evidence obtained in this study clearly shows that the CD4+ cell population is indispensable for ulceration in leishmaniasis lesions of SCID mice.

Original languageEnglish
Pages (from-to)4574-4577
Number of pages4
JournalInfection and Immunity
Volume68
Issue number8
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Cutaneous Leishmaniasis
Ulcer
Severe Combined Immunodeficiency
Leishmania
Leishmaniasis
Population
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Terabe, M., Kuramochi, T., Ito, M., Hatabu, T., Sanjoba, C., Chang, K. P., ... Matsumoto, Y. (2000). CD4+ cells are indispensable for ulcer development in murine cutaneous leishmaniasis. Infection and Immunity, 68(8), 4574-4577. https://doi.org/10.1128/IAI.68.8.4574-4577.2000

CD4+ cells are indispensable for ulcer development in murine cutaneous leishmaniasis. / Terabe, Masaki; Kuramochi, Takashi; Ito, Mamoru; Hatabu, Toshimitsu; Sanjoba, Chizu; Chang, Kwang Poo; Onodera, Takashi; Matsumoto, Yoshitsugu.

In: Infection and Immunity, Vol. 68, No. 8, 2000, p. 4574-4577.

Research output: Contribution to journalArticle

Terabe, M, Kuramochi, T, Ito, M, Hatabu, T, Sanjoba, C, Chang, KP, Onodera, T & Matsumoto, Y 2000, 'CD4+ cells are indispensable for ulcer development in murine cutaneous leishmaniasis', Infection and Immunity, vol. 68, no. 8, pp. 4574-4577. https://doi.org/10.1128/IAI.68.8.4574-4577.2000
Terabe, Masaki ; Kuramochi, Takashi ; Ito, Mamoru ; Hatabu, Toshimitsu ; Sanjoba, Chizu ; Chang, Kwang Poo ; Onodera, Takashi ; Matsumoto, Yoshitsugu. / CD4+ cells are indispensable for ulcer development in murine cutaneous leishmaniasis. In: Infection and Immunity. 2000 ; Vol. 68, No. 8. pp. 4574-4577.
@article{2e3d3a266572469cb3e02b84df453c6d,
title = "CD4+ cells are indispensable for ulcer development in murine cutaneous leishmaniasis",
abstract = "One of the most characteristic clinical features in cutaneous leishmaniasis is the development of nodules followed by ulcerations at the site of infection. Leishmania amazonensis-infected mice show similar ulcerative lesions. Leishmania-infected severe combined immunodeficiency (SCID) mice, however, have been shown to develop nonulcerative nodules. In the present study, the roles of T cells in ulceration were examined using SCID mice in cell reconstitution experiments. After development of nonulcerative nodules, SCID mice were inoculated with splenocytes from either Leishmania-infected or naive immunocompetent mice, resulting in ulceration in all mice. When naive splenocytes were depleted of CD4+, CD8+, or B220+ cell populations and the remaining cells were injected into Leishmania- infected SCID mice after the development of nodules, only SCID mice inoculated with splenocytes depleted of CD4+ cells did not show ulceration. The evidence obtained in this study clearly shows that the CD4+ cell population is indispensable for ulceration in leishmaniasis lesions of SCID mice.",
author = "Masaki Terabe and Takashi Kuramochi and Mamoru Ito and Toshimitsu Hatabu and Chizu Sanjoba and Chang, {Kwang Poo} and Takashi Onodera and Yoshitsugu Matsumoto",
year = "2000",
doi = "10.1128/IAI.68.8.4574-4577.2000",
language = "English",
volume = "68",
pages = "4574--4577",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - CD4+ cells are indispensable for ulcer development in murine cutaneous leishmaniasis

AU - Terabe, Masaki

AU - Kuramochi, Takashi

AU - Ito, Mamoru

AU - Hatabu, Toshimitsu

AU - Sanjoba, Chizu

AU - Chang, Kwang Poo

AU - Onodera, Takashi

AU - Matsumoto, Yoshitsugu

PY - 2000

Y1 - 2000

N2 - One of the most characteristic clinical features in cutaneous leishmaniasis is the development of nodules followed by ulcerations at the site of infection. Leishmania amazonensis-infected mice show similar ulcerative lesions. Leishmania-infected severe combined immunodeficiency (SCID) mice, however, have been shown to develop nonulcerative nodules. In the present study, the roles of T cells in ulceration were examined using SCID mice in cell reconstitution experiments. After development of nonulcerative nodules, SCID mice were inoculated with splenocytes from either Leishmania-infected or naive immunocompetent mice, resulting in ulceration in all mice. When naive splenocytes were depleted of CD4+, CD8+, or B220+ cell populations and the remaining cells were injected into Leishmania- infected SCID mice after the development of nodules, only SCID mice inoculated with splenocytes depleted of CD4+ cells did not show ulceration. The evidence obtained in this study clearly shows that the CD4+ cell population is indispensable for ulceration in leishmaniasis lesions of SCID mice.

AB - One of the most characteristic clinical features in cutaneous leishmaniasis is the development of nodules followed by ulcerations at the site of infection. Leishmania amazonensis-infected mice show similar ulcerative lesions. Leishmania-infected severe combined immunodeficiency (SCID) mice, however, have been shown to develop nonulcerative nodules. In the present study, the roles of T cells in ulceration were examined using SCID mice in cell reconstitution experiments. After development of nonulcerative nodules, SCID mice were inoculated with splenocytes from either Leishmania-infected or naive immunocompetent mice, resulting in ulceration in all mice. When naive splenocytes were depleted of CD4+, CD8+, or B220+ cell populations and the remaining cells were injected into Leishmania- infected SCID mice after the development of nodules, only SCID mice inoculated with splenocytes depleted of CD4+ cells did not show ulceration. The evidence obtained in this study clearly shows that the CD4+ cell population is indispensable for ulceration in leishmaniasis lesions of SCID mice.

UR - http://www.scopus.com/inward/record.url?scp=0033914835&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033914835&partnerID=8YFLogxK

U2 - 10.1128/IAI.68.8.4574-4577.2000

DO - 10.1128/IAI.68.8.4574-4577.2000

M3 - Article

C2 - 10899857

AN - SCOPUS:0033914835

VL - 68

SP - 4574

EP - 4577

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 8

ER -