CD4-CD8 - T cell receptor αβ T cells: Generation of an in vitro major histocompatability complex class I specific cytotoxic T lymphocyte response and allogeneic tumor rejection

Masahiro Mieno, Ryuichiro Suto, Yuichi Obata, Heiichiro Udono, Toshitada Takahashi, Hiroshi Shiku, Eiichi Nakayama

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The generation of an in vitro major histocompatibility complex class I specific response of CD4CD8 T cell receptor (TCR) αβ cytotoxic T lymphocytes (CTL) and their allogeneic tumor rejection were investigated. Inocula ofBALBRLo’ 1 were rejected in C57BL/6 (B6) mice treated with minimum essential medium (MEM) (control), anti-L3T4 (CD4) monoclonal antibody (mAb) or anti-Lyt-2.2 (CD8) mAb and CTL against the tumor were generated in vitro. No rejection and no induction of CTL were observed in B6 mice treated with anti-L3T4 (CD4) plus anti-Lyt-2.2 (CD8) mAb. CTL with the classical Thy-1+CD3+CD4 CD8+ TCR αβ phenotype were generated in mixed lymphocyte tumor cell culture (MLTC) spleen cells from B6 mice treated with MEM (control) or anti-L3T4 (CD4) mAb, whereas CTL with an unusual Thy-1+CD3+CD4 CD8 TCR αβ phenotype were generated in MLTC spleen cells from anti-Lyt-2.2 (CD8) mAb-treated B6 mice. Both types of CTL were reactive with both H-2Kd and Dd (Ld) class I antigen. These findings suggest that when CD4+ cells were blocked by anti- L3T4 (CD4) mAb, CD8+ CTL mediated rejection, and when CD8+ cells were blocked by anti-Lyt-2.2 (CD8) mAb, CD4+ cells were capable of mediating rejection, although less efficiently than CD8+ cells, by inducing CD4 CD8 TCR αβ CTL. The finding that adoptive transfer of CD4 and CD8-depleted MLTC spleen cells, obtained from anti-Lyt-2.2 (CD8) mAbtreated B6 mice that had rejected BALBRLa 1, resulted in rejection of BALBRLa 1 inoculated into B6 nu/nu mice confirmed the above notion. CTL clones with the CD4 CD8 TCR αβ phenotype specific for Ld were established.

Original languageEnglish
Pages (from-to)193-201
Number of pages9
JournalJournal of Experimental Medicine
Issue number1
Publication statusPublished - Jul 1 1991


ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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