CD4- and CD56-positive T-cell line, MTA, established from natural killer-like T-cell leukemia/lymphoma

Nobuhiko Emi, Akihiro Abe, Masanobu Kasai, Akio Kohno, Mitsune Tanimoto, Hiroshi Kimura, Kohei Kawashima, Masafumi Ito, Naoyoshi Mori, Hidehiko Saito

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have established a T-cell line, MTA, from the peripheral blasts of a patient with natural killer (NK)-like T-cell leukemia/lymphoma. The MTA cell displays a T-cell and NK-cell phenotype (CD2+, CD3+, CD4+, CD56+) identical to freshly isolated leukemic blasts from the patient. This cell line shows clonal T-cell receptor rearrangement and a distinguishable character by light microscopy with May-Grünwald Giemsa staining. G-banding analysis showed that the MTA cells had a karyotype of 94(4N), XXXX, add (1) (p36), del (5) (q14q23), add (17) (p11), add (19) (q13). However, unlike NK malignancy, we could not show a direct pathogenic role for Epstein-Barr virus (EBV) with EBV-encoded small RNA and polymerase chain reaction analysis. The MTA cell line is a novel cell line with which to study NK-like T-cell ontogenecity.

Original languageEnglish
Pages (from-to)180-185
Number of pages6
JournalInternational Journal of Hematology
Volume69
Issue number3
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Pemetrexed
T-Cell Leukemia
Natural Killer T-Cells
T-Cell Lymphoma
T-Lymphocytes
Cell Line
Human Herpesvirus 4
DNA-Directed RNA Polymerases
T-Cell Antigen Receptor
Karyotype
Natural Killer Cells
Microscopy
Staining and Labeling
Phenotype
Light
Polymerase Chain Reaction
Neoplasms

Keywords

  • CD56
  • Epstein-Barr virus
  • Natural killer-like T-cell leukemia/lymphoma

ASJC Scopus subject areas

  • Hematology

Cite this

Emi, N., Abe, A., Kasai, M., Kohno, A., Tanimoto, M., Kimura, H., ... Saito, H. (1999). CD4- and CD56-positive T-cell line, MTA, established from natural killer-like T-cell leukemia/lymphoma. International Journal of Hematology, 69(3), 180-185.

CD4- and CD56-positive T-cell line, MTA, established from natural killer-like T-cell leukemia/lymphoma. / Emi, Nobuhiko; Abe, Akihiro; Kasai, Masanobu; Kohno, Akio; Tanimoto, Mitsune; Kimura, Hiroshi; Kawashima, Kohei; Ito, Masafumi; Mori, Naoyoshi; Saito, Hidehiko.

In: International Journal of Hematology, Vol. 69, No. 3, 1999, p. 180-185.

Research output: Contribution to journalArticle

Emi, N, Abe, A, Kasai, M, Kohno, A, Tanimoto, M, Kimura, H, Kawashima, K, Ito, M, Mori, N & Saito, H 1999, 'CD4- and CD56-positive T-cell line, MTA, established from natural killer-like T-cell leukemia/lymphoma', International Journal of Hematology, vol. 69, no. 3, pp. 180-185.
Emi, Nobuhiko ; Abe, Akihiro ; Kasai, Masanobu ; Kohno, Akio ; Tanimoto, Mitsune ; Kimura, Hiroshi ; Kawashima, Kohei ; Ito, Masafumi ; Mori, Naoyoshi ; Saito, Hidehiko. / CD4- and CD56-positive T-cell line, MTA, established from natural killer-like T-cell leukemia/lymphoma. In: International Journal of Hematology. 1999 ; Vol. 69, No. 3. pp. 180-185.
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AU - Kimura, Hiroshi

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