CCN2/CTGF binds the small leucine rich proteoglycan protein Tsukushi

Kunimasa Ohta, Eriko Aoyama, Shah Adil Ishtiyaq Ahmad, Naofumi Ito, Mohammad Badrul Anam, Satoshi Kubota, Masaharu Takigawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Extracellular molecules coordinate the multiple signaling pathways spatiotemporally to exchange information between cells during development. Understanding the regulation of these signal molecule-dependent pathways elucidates the mechanism of intercellular crosstalks. CCN2/CTGF is one of the CCN family members that binds BMP2, fibronectin, aggrecan, FGFR2 - regulating cartilage and bone formation, angiogenesis, wound repair etc. Tsukushi (TSK), which belongs to the Small Leucine-Rich Proteoglycan (SLRP) family, binds nodal/Vg1/TGF-β1, BMP4/chordin, Delta, FGF8, Frizzled4, and is involved in the early body formation, bone growth, wound healing, retinal stem cell regulation etc. These two secreted molecules are expressed in similar tissues and involved in several biological events by functioning as extracellular signaling modulators. Here, we examine the molecular interaction between CCN2 and TSK biochemically. Co-precipitation assay and Surface Plasmon Resonance measurement showed their direct binding with the Kd value 15.3 nM. Further, the Solid-phase Binding Assay indicated that TSK binds to IGFBP and CT domains of CCN2. Our data suggest that CCN2 and TSK exert their function together in the body formation.

Original languageEnglish
JournalJournal of Cell Communication and Signaling
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Aggrecans
Insulin-Like Growth Factor Binding Proteins
Surface Plasmon Resonance
Bone Development
Proteoglycans
Fibronectins
Osteogenesis
Leucine
Wound Healing
Cartilage
Stem Cells
Molecules
Assays
Wounds and Injuries
Bone
Proteins
Molecular interactions
Surface plasmon resonance
Coprecipitation
Crosstalk

Keywords

  • CCN2/CTGF
  • SLRP
  • Soluble molecule
  • Tsukushi
  • Vertebrate development

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

CCN2/CTGF binds the small leucine rich proteoglycan protein Tsukushi. / Ohta, Kunimasa; Aoyama, Eriko; Ahmad, Shah Adil Ishtiyaq; Ito, Naofumi; Anam, Mohammad Badrul; Kubota, Satoshi; Takigawa, Masaharu.

In: Journal of Cell Communication and Signaling, 01.01.2018.

Research output: Contribution to journalArticle

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AU - Ohta, Kunimasa

AU - Aoyama, Eriko

AU - Ahmad, Shah Adil Ishtiyaq

AU - Ito, Naofumi

AU - Anam, Mohammad Badrul

AU - Kubota, Satoshi

AU - Takigawa, Masaharu

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