CCN2 as a novel molecule supporting energy metabolism of chondrocytes

Aya Maeda-Uematsu, Satoshi Kubota, Harumi Kawaki, Kazumi Kawata, Yoshiaki Miyake, Takako Hattori, Takashi Nishida, Norifumi Moritani, Karen M. Lyons, Seiji Iida, Masaharu Takigawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

CCN2/connective tissue growth factor (CTGF) is a unique molecule that promotes both chondrocytic differentiation and proliferation through its matricellular interaction with a number of extracellular biomolecules. This apparently contradictory functional property of CCN2 suggests its certain role in basic cellular activities such as energy metabolism, which is required for both proliferation and differentiation. Comparative metabolomic analysis of costal chondrocytes isolated from wild-type and Ccn2-null mice revealed overall impaired metabolism in the latter. Among the numerous metabolites analyzed, stable reduction in the intracellular level of ATP, GTP, CTP, or UTP was observed, indicating a profound role of CCN2 in energy metabolism. Particularly, the cellular level of ATP was decreased by more than 50% in the Ccn2-null chondrocytes. The addition of recombinant CCN2 (rCCN2) to cultured Ccn2-null chondrocytes partly redeemed the cellular ATP level attenuated by Ccn2 deletion. Next, in order to investigate the mechanistic background that mediates the reduction in ATP level in these Ccn2-null chondrocytes, we performed transcriptome analysis. As a result, several metabolism-associated genes were found to have been up-regulated or down-regulated in the mutant mice. Up-regulation of a number of ribosomal protein genes was observed upon Ccn2 deletion, whereas a few genes required for aerobic and anaerobic ATP production were down-regulated in the Ccn2-null chondrocytes. Among such genes, reduction in the expression of the enolase 1 gene was of particular note. These findings uncover a novel functional role of CCN2 as a metabolic supporter in the growth-plate chondrocytes, which is required for skeletogenesis in mammals.

Original languageEnglish
Pages (from-to)854-865
Number of pages12
JournalJournal of Cellular Biochemistry
Volume115
Issue number5
DOIs
Publication statusPublished - 2014

Fingerprint

Chondrocytes
Energy Metabolism
Genes
Adenosine Triphosphate
Molecules
Metabolism
Connective Tissue Growth Factor
Cytidine Triphosphate
Uridine Triphosphate
Mammals
Phosphopyruvate Hydratase
Ribosomal Proteins
Biomolecules
Metabolites
Guanosine Triphosphate
Metabolomics
Growth Plate
Gene Expression Profiling
Up-Regulation

Keywords

  • CARTILAGE
  • CCN2
  • CHONDROCYTES
  • CTGF
  • METABOLISM

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

CCN2 as a novel molecule supporting energy metabolism of chondrocytes. / Maeda-Uematsu, Aya; Kubota, Satoshi; Kawaki, Harumi; Kawata, Kazumi; Miyake, Yoshiaki; Hattori, Takako; Nishida, Takashi; Moritani, Norifumi; Lyons, Karen M.; Iida, Seiji; Takigawa, Masaharu.

In: Journal of Cellular Biochemistry, Vol. 115, No. 5, 2014, p. 854-865.

Research output: Contribution to journalArticle

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