Caveolin-1 expression by means of p38β mitogen-activated protein kinase mediates the antiproliferative effect of carbon monoxide

Hong Pyo Kim, Xue Wang, Atsunori Nakao, Sung Il Kim, Noriko Murase, Mary E. Choi, Stefan W. Ryter, Augustine M K Choi

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

During vascular injury, the proliferation and migration of smooth muscle cells leads to characteristic neointima formation, which can be exacerbated by genetic depletion of caveolin-1 or heme oxygenase 1 (HO-1), and inhibited by carbon monoxide (CO), a by-product of heme oxygenase 1 activity. CO inhibited smooth muscle cell proliferation by activating p38 mitogen-activated protein kinase (MAPK) and p21Waf1/ciP1. Exposure to CO increased caveolin-1 expression in neointimal lesions of injured aorta and in vitro by activating guanylyl cyclase and p38 MAPK. p38β-/- fibroblasts did not induce caveolin-1 in response to CO, and exhibited a diminished basal caveolin-1 expression, which was restored by p38/B gene transfer. p38/3 WIAPK down-regulated extracellular signal-regulated protein kinase 1/2 (ERK-1/2), which can repress caveolin-1 transcription. Genetic depletion of caveolin-1 abolished the antiproliferative effect of CO. Thus, we demonstrate that CO, by activating p38/3 MAPK, up-regulates caveolin-1, which acts as a tumor suppressor protein that mediates the growth inhibitory properties of this gas.

Original languageEnglish
Pages (from-to)11319-11324
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number32
DOIs
Publication statusPublished - Aug 9 2005
Externally publishedYes

Fingerprint

Caveolin 1
p38 Mitogen-Activated Protein Kinases
Carbon Monoxide
Heme Oxygenase-1
Smooth Muscle Myocytes
Tumor Suppressor Proteins
Neointima
Mitogen-Activated Protein Kinase 3
Guanylate Cyclase
Vascular System Injuries
Protein Kinases
Aorta
Up-Regulation
Fibroblasts
Gases
Cell Proliferation
Growth

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Caveolin-1 expression by means of p38β mitogen-activated protein kinase mediates the antiproliferative effect of carbon monoxide. / Kim, Hong Pyo; Wang, Xue; Nakao, Atsunori; Kim, Sung Il; Murase, Noriko; Choi, Mary E.; Ryter, Stefan W.; Choi, Augustine M K.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 32, 09.08.2005, p. 11319-11324.

Research output: Contribution to journalArticle

Kim, Hong Pyo ; Wang, Xue ; Nakao, Atsunori ; Kim, Sung Il ; Murase, Noriko ; Choi, Mary E. ; Ryter, Stefan W. ; Choi, Augustine M K. / Caveolin-1 expression by means of p38β mitogen-activated protein kinase mediates the antiproliferative effect of carbon monoxide. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 32. pp. 11319-11324.
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