Causes of the production of multiple forms of β-galactosidase by Bacillus circulans

Jingyuan Song, Kiriko Abe, Hiroyuki Imanaka, Koreyoshi Imamura, Masashi Minoda, Shotaro Yamaguchi, Kazuhiro Nakanishi

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The presence of multiple types of β-galactosidases in a commercial enzyme preparation from Bacillus circulans ATCC 31382 and differences in their trans-galactosylation activity were investigated. Four β-gal-actosidases, β-Gal-A, β-Gal-B, β-Gal-C, and β-Gal-D, which were immunologically homologous, were isolated and characterized. The N-terminal amino acid sequences of all of the enzymes were identical and biochemical characteristics were similar, except for galactooligosaccharide production. β-Gal-B, β-Gal-C, and jS-Gal-D produced mainly tri- and tetra saccharides at maximum yields of 20-30 and 9-12%, while β-Gal-A produced trisaccharide with 7% with 5% lactose as substrate. The Lineweaver-Burk plots for all of the enzymes, except for β-Gal-A, showed biphasic behavior. β-Gal-A was truncated to yield multiple β-galactosidases by treatment with protease isolated from the culture broth of B. circulans. Treatment of β-Gal-A with trypsin yielded an active 91-kDa protein composed of 21-kDa and 70-kDa proteins with characteristics similar to those for β-Gal-D.

Original languageEnglish
Pages (from-to)268-278
Number of pages11
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number2
DOIs
Publication statusPublished - 2011

Fingerprint

Galactosidases
Bacilli
Bacillus
Enzymes
Trisaccharides
Lactose
Trypsin
Amino Acid Sequence
Proteins
Peptide Hydrolases
Amino Acids
Substrates

Keywords

  • β-galactosidase
  • Bacillus circulans
  • Galactooligosaccharide
  • Trans-galactosylation

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Causes of the production of multiple forms of β-galactosidase by Bacillus circulans. / Song, Jingyuan; Abe, Kiriko; Imanaka, Hiroyuki; Imamura, Koreyoshi; Minoda, Masashi; Yamaguchi, Shotaro; Nakanishi, Kazuhiro.

In: Bioscience, Biotechnology and Biochemistry, Vol. 75, No. 2, 2011, p. 268-278.

Research output: Contribution to journalArticle

Song, Jingyuan ; Abe, Kiriko ; Imanaka, Hiroyuki ; Imamura, Koreyoshi ; Minoda, Masashi ; Yamaguchi, Shotaro ; Nakanishi, Kazuhiro. / Causes of the production of multiple forms of β-galactosidase by Bacillus circulans. In: Bioscience, Biotechnology and Biochemistry. 2011 ; Vol. 75, No. 2. pp. 268-278.
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